Two multivariate calibration methods, partial least squares (PLS-1) and principal component regression (PCR), were effectively used to determine the concentrations of three dyes in mixtures through ultraviolet-visible absorption spectrophotometry.
The calibration models underwent various evaluation methods, including internal, cross-validation, and external validation, showing high recovery rates (93.5-103.1%) for synthetic mixtures of the dyes and commercial products.
Studies on repeatability and reproducibility demonstrated no significant differences among dye standards at a 95% confidence level, affirming the reliability of the calibration models.