Screening Algal and Cyanobacterial Extracts to Identify Potential Substitutes for Fetal Bovine Serum in Cellular Meat Cultivation.

Cultured meat technology is a form of cellular agriculture where meat is produced from animal cells grown in a lab, instead of raising and slaughtering animals. This technology relies heavily on fetal bovine serum (FBS) in cell media; hence, production is costly and contributes significantly to ammonia and greenhouse gas emissions. Achieving the successful commercialization of cell-cultured food requires the critical resolution of manufacturing cost and safety concerns.

Description of fundatrix and sexual morphs of the Asian apricot aphid, Myzus mumecola (Matsumura, 1917) (Hemiptera: Aphididae).

Fundatrix and sexual morphs of the Asian apricot aphid, Myzus (Myzus) mumecola (Matsumura, 1917) are described from Serbia with original, clarified slide mounts, and illustrations. The morphological identification of sexual morphs was corroborated through COI barcode sequence analysis. The subgeneric position of this species is discussed and the transfer to subgenus Nectarosiphon is proposed.

Bioassay-guided evaluation of antimicrobial properties and profile of bioactive compounds from leaf, peel and mesocarp of four apple cultivars (Malus domestica Borkh.) grown in Serbia: Application of HPTLC-EDA and UHPLC Q-ToF MS techniques.

The aim of this study was to evaluate the antimicrobial properties and profile of bioactive compounds from mesocarp, peel and leaves of four autochthonous apple cultivars against human pathogens, Escherichia coli, Staphylococcus aureus and Bacillus subtilis and the apple pathogen Erwinia amylovora by direct detection on HPTLC plates and subsequent chemometric analysis. UHPLC Q-ToF MS was used for detailed characterization of the bioactive compounds with antimicrobial properties. Leaf extracts showed the highest antibacterial activity against all bacterial strains, followed by peel extracts, while the mesocarp extracts showed only weak and selective inhibition zones for E.

Molecular characterization of wheat dwarf virus isolates from Serbia based on complete genome sequences.

Introduction: Wheat dwarf virus (WDV), the species of the genus in the family , is a cereal virus commonly detected in several European, African and Asian countries that causes economic losses.

Methods: In the spring of 2019, a severe outbreak of wheat dwarfing and yellowing was observed in many winter wheat crops across Serbia. A total of 161 samples were tested for the presence of WDV and other common wheat viruses using double-antibody sandwich enzyme-linked immunosorbent assay (DAS-ELISA).

The adsorption of chlorpyrifos and malathion under environmentally relevant conditions using biowaste carbon materials.

Water bodies face persistent contamination from organophosphorus pesticides like chlorpyrifos and malathion, which pose substantial environmental and health hazards due to their toxicity and resilience in ecosystems. This study explores the potential of spent coffee grounds, a common agricultural byproduct, as an eco-friendly adsorbent for eliminating these pesticides from polluted water. Spent coffee grounds underwent carbonization at 400 °C and various activation treatments using KOH, HPO, CO, and their combinations.

Alternaria species on revealed as potential inoculum source for solanaceous crops in Serbia.

Alternaria pathogens are a global agronomic challenge affecting the health of Solanaceae crops. Crop debris, seeds, and perennial weeds are potential inoculum reservoirs, but knowledge on their relative importance remains limited. Plants of Convolvulus arvensis showing early blight and brown leaf spot symptoms were collected from in and around potato and tomato fields in Serbia, grown both in open conditions and in tunnels, in the late season of 2021 - 2022.

Effects of mining activities and municipal wastewaters on element accumulation and integrated biomarker responses of the European chub (Squalius cephalus).

This study aimed to determine concentrations of 29 elements in the gills and liver as well as biomarker response in gills, liver, and blood of European chub from Pek River (exposed to long-term mining activities), and to compare these findings with individuals from Ibar River (influenced by emission of treated municipal wastewater) and Kruščica reservoir (source of drinking water) using inductively-coupled plasma optical emission spectrometry (ICP-OES). The metal pollution index (MPI) was also calculated. Supporting analyses for the detection of the municipal wastewater presence at investigated localities included analyses of microbiological indicators (total coliforms and Escherichia coli) of faecal pollution.

The phytochemical composition and unexplored potential of Australian native plants for application in physical activity-related muscle recovery and inflammation: a literature review.

Native plants are adaptable in various environmental conditions in part through the production of unique phytochemicals which may have beneficial effects on human health. Native Australian fruits contain higher phytochemical and antioxidant levels than most Western fruits, suggesting potential for greater health benefits arising from their consumption. These beneficial effects, in turn, may be mediated by the inhibition of inflammatory pathways as well as oxidative stress the regulation of reactive oxygen (ROS) and/or nitrogen (RNS) species levels.

The ongoing range expansion of the invasive oak lace bug across Europe: current occurrence and potential distribution under climate change.

In recent years, the oak lace bug, Corythucha arcuata, has emerged as a significant threat to European oak forests. This species, native to North America, has in the last two decades rapidly extended its range in Europe, raising concerns about its potential impact on the continent's invaluable oak populations. To address this growing concern, we conducted an extensive study to assess the distribution, colonization patterns, and potential ecological niche of the oak lace bug in Europe.

Geographical variations, prevalence, and molecular dynamics of fastidious phloem-limited pathogens infecting sugar beet across Central Europe.

In Europe, two fastidious phloem-limited pathogens, 'Candidatus Phytoplasma solani' (16SrXII-A) and 'Candidatus Arsenophonus phytopathogenicus', are associated with rubbery taproot disease (RTD) and syndrome basses richesses (SBR) of sugar beet, respectively. Both diseases can significantly reduce yield, especially when accompanied by root rot fungi. This study investigates the presence, geographic distribution and genetic traits of fastidious pathogens and the accompanying fungus, Macrophomina phaseolina, found on sugar beet across four geographically separated plains spanning seven countries in Central Europe.

Application of parallel artificial membrane permeability assay technique and chemometric modeling for blood-brain barrier permeability prediction of protein kinase inhibitors.

This study aims to investigate the passive diffusion of protein kinase inhibitors through the blood-brain barrier (BBB) and to develop a model for their permeability prediction. We used the parallel artificial membrane permeability assay to obtain logPe values of each of 34 compounds and calculated descriptors for these structures to perform quantitative structure-property relationship modeling, creating different regression models. The logPe values have been calculated for all 34 compounds.

Taming the terminological tempest in invasion science.

Standardised terminology in science is important for clarity of interpretation and communication. In invasion science - a dynamic and rapidly evolving discipline - the proliferation of technical terminology has lacked a standardised framework for its development. The result is a convoluted and inconsistent usage of terminology, with various discrepancies in descriptions of damage and interventions.

Harmonised statistics and maps of forest biomass and increment in Europe.

Forest biomass is an essential resource in relation to the green transition and its assessment is key for the sustainable management of forest resources. Here, we present a forest biomass dataset for Europe based on the best available inventory and satellite data, with a higher level of harmonisation and spatial resolution than other existing data. This database provides statistics and maps of the forest area, biomass stock and their share available for wood supply in the year 2020, and statistics on gross and net volume increment in 2010-2020, for 38 European countries.

Climate acts as an environmental filter to plant pathogens.

Climate shapes the distribution of plant-associated microbes such as mycorrhizal and endophytic fungi. However, the role of climate in plant pathogen community assembly is less understood. Here, we explored the role of climate in the assembly of Phytophthora communities at >250 sites along a latitudinal gradient from Spain to northern Sweden and an altitudinal gradient from the Spanish Pyrenees to lowland areas.

First Report of Causing Onion Stemphylium Leaf Blight in Mauritius.

In August 2021, severe leaf blight symptoms were observed on onion ( L. cvs Francia and Askari F1 hybrid) in commercial fields located in Mauritius, namely La Forêt (20°19'56.1"S57°30'04.

Phytochemistry and Micromorphology of Epicuticular Waxes in Four Juniper Species With Fragmented Distribution in the Balkans.

The cuticle is important in the interaction between the plant and its environment, especially in the dry areas. Four species of junipers from the section Sabina wild growing in the Balkans were selected to study leaf wax composition using GC/MS and GC-FID and its surface morphology under SEM to understand the correlation between the distribution and/or habitat of these species and their cuticles. SEM micrographs showed continuous, smooth epicuticular layers with crusts in all species but with a species-specific distribution of different 3D crystalloid types and different cuticle thickness.

Ultrasensitive Quantification of Crustacean Tropomyosin by Immuno-PCR.

Tropomyosin is the major and predominant allergen among shellfish. This study developed an ultrasensitive immuno-PCR method for the quantification of crustacean tropomyosin in foods. The method couples sandwich ELISA with the real-time PCR (rtPCR) amplification of marker DNAs.

Investigation of Anticholinesterase Activity of Chemically Characterised Hieracium s. str. Methanol Extracts and Their Selected Metabolites.

The composition and anticholinesterase activity of the dried MeOH extracts of Hieracium scheppigianum and H. naegelianum underground parts (rhizomes and roots), as well as the anticholinesterase activity of the dried, previously chemically characterised MeOH extracts of the flowering aerial parts of these two and 26 other Hieracium species in the strict sense (s. str.

Optimization of solid-state fermentation for enhanced production of pectinolytic complex by Aspergillus tubingensis FAT43 and its application in fruit juice processing.

The main goal of this study was to examine the efficiency of a newly isolated fungus from quince, Aspergillus tubingensis FAT43, to produce the pectinolytic complex using agricultural and industrial waste as the substrate for solid state fermentation. Sugar beet pulp was the most effective substrate inducer of pectinolytic complex synthesis out of all the waste residues examined. For endo-pectinolytic and total pectinolytic activity, respectively, statistical optimization using Placked-Burman Design and Optimal (Custom) Design increased production by 2.

First Report of Beet Yellows Virus Causing Virus Yellows in Sugar Beet in Serbia.

Several viruses have been described to infect sugar beet ( var. L.), but virus yellows disease is one of the most important diseases in many sugar beet growing areas.

Empirical and process-based models predict enhanced beech growth in European mountains under climate change scenarios: A multimodel approach.

Process-based models and empirical modelling techniques are frequently used to (i) explore the sensitivity of tree growth to environmental variables, and (ii) predict the future growth of trees and forest stands under climate change scenarios. However, modelling approaches substantially influence predictions of the sensitivity of trees to environmental factors. Here, we used tree-ring width (TRW) data from 1630 beech trees from a network of 70 plots established across European mountains to build empirical predictive growth models using various modelling approaches.