3D Time-lapse Imaging and Quantification of Mitochondrial Dynamics.

We present a 3D time-lapse imaging method for monitoring mitochondrial dynamics in living HeLa cells based on photothermal optical coherence microscopy and using novel surface functionalization of gold nanoparticles. The biocompatible protein-based biopolymer coating contains multiple functional groups which impart better cellular uptake and mitochondria targeting efficiency. The high stability of the gold nanoparticles allows continuous imaging over an extended time up to 3000 seconds without significant cell damage.

High-Throughput and Low-Cost Analysis of Trace Volatile Phthalates in Seafood by Online Coupling of Monolithic Capillary Adsorbent with GC-MS.

A simple, sensitive, and high-throughput method was developed for the determination of six volatile phthalate esters-dimethyl phthalate (DMP), diethyl phthalate (DEP), dibutyl phthalate (DBP), benzylbutyl phthalate (BBP), di(2-ethylhexyl) phthalate (DEHP), and di-n-octyl phthalate (DnOP)-in seafood samples by using monolith adsorbent in a capillary coupled to a gas chromatography-mass spectrometry (GC-MS) system. The freeze-dried samples were subjected to an ultrasonication with hexane, followed by vortex mixing. The liquid extract was quantitatively determined by a direct application to an online silica monolith capillary adsorbent coupled with a gas chromatograph with mass spectrometric detection.

MyHip: supporting planning and surgical guidance for a better total hip arthroplasty : A pilot study.

Purpose: Total hip arthroplasty (THA) aims to restore patient mobility by providing a pain-free and stable artificial joint. A successful THA depends on the planning and its execution during surgery. Both tasks rely on the experience of the surgeon to understand the complex biomechanical behavior of the hip.

Views of healthcare professionals dealing with legal termination of pregnancy up to 12 WA in French-speaking Switzerland.

Background: In 2002, by popular vote, Swiss citizens accepted to legalise termination of pregnancy (TOP), up to the 12th week of amenorrhoea (WA). As a result, the cantons formulated rules of application. In 2002, medical TOP was authorised.

Clinical course in women undergoing termination of pregnancy within the legal time limit in French-speaking Switzerland.

Background: In 2002, Swiss citizens voted to accept new laws legalising the termination of pregnancy (TOP) up to 12th week of pregnancy. As a result the cantons formulated rules of implementation. Health institutions then had to modify their procedures and practices.