Crucial aspects for maintaining rAAV stability.

The storage of rAAV vectors for gene therapy applications is critical for ensuring a constant product quality and defined amount of medication at the time of administration. Therefore, we determined the influence of different storage conditions on the physicochemical and biological properties of rAAV8 and rAAV9 preparations. Particular attention was paid to short-term storage, which plays a crucial role in both the manufacturing process and in clinical applications.

Nurses' experiences with ad-hoc patient education in an Austrian acute care setting - A qualitative multicentric study.

Objective: This paper aims to explore nurses' experiences with ad-hoc patient education (AHPE) in an acute inpatient setting.

Methods: We conducted nine focus groups with 34 nurses. Data was analysed using thematic analysis and the social-ecological model (SEM).

A Digital Box and Block Test for Hand Dexterity Measurement: Instrument Validation Study.

Background: The Box and Block Test (BBT) measures unilateral gross manual dexterity and is widely used in clinical settings with a wide range of populations, including older people and clients with neurological disorders.

Objective: In this study, we present a newly developed digitized version of the BBT, called the digital BBT (dBBT). The physical design is similar to the original BBT, but the dBBT contains digital electronics that automate the test procedure, timing, and score measurement.

Group-based circuit training to improve mobility after stroke: a cross-sectional survey of German and Austrian physical therapists in outpatient settings.

Background: A contextual transferability analysis identified group-based circuit training (GCT) as an optimal intervention in German and Austrian outpatient physical therapy to improve mobility post-stroke. GCT incorporates task-oriented, high-repetitive, balance, aerobic and strength training and allows for increased therapy time without increasing personnel.

Objective: To determine the extent to which German and Austrian physical therapists (PTs) use GCT and its components in the outpatient treatment of stroke-related mobility deficits and to identify factors associated with using GCT components.

7T HR FID-MRSI Compared to Amino Acid PET: Glutamine and Glycine as Promising Biomarkers in Brain Tumors.

(1) Background: Recent developments in 7T magnetic resonance spectroscopic imaging (MRSI) made the acquisition of high-resolution metabolic images in clinically feasible measurement times possible. The amino acids glutamine (Gln) and glycine (Gly) were identified as potential neuro-oncological markers of importance. For the first time, we compared 7T MRSI to amino acid PET in a cohort of glioma patients.

Environmental and economic assessment of food-packaging systems with a focus on food waste. Case study on tomato ketchup.

In this paper, a sustainability evaluation method for food-packaging systems is proposed. First, food waste due to poor emptiability was determined. Then, these quantities were included in life cycle assessments (LCA) and life cycle costing (value added, VA) of the products.

IGF1 inclusion bodies: A QbD based process approach for efficient USP as well as early DSP unit operations.

E. coli is an attractive host organism for strong recombinant protein expression. It expresses products either as soluble protein or as inclusion bodies (IB).

Plant-derived protein bodies as delivery vehicles for recombinant proteins into mammalian cells.

The encapsulation of biopharmaceuticals into micro- or nanoparticles is a strategy frequently used to prevent degradation or to achieve the slow release of therapeutics and vaccines. Protein bodies (PBs), which occur naturally as storage organelles in seeds, can be used as such carrier vehicles. The fusion of the N-terminal sequence of the maize storage protein, γ-zein, to other proteins is sufficient to induce the formation of PBs, which can be used to bioencapsulate recombinant proteins directly in the plant production host.

Adsorptive filtration: A case study for early impurity reduction in an Escherichia coli production process.

Primary recovery of intracellular products from Escherichia coli requires cell disruption which leads to a massive release of process-related impurities burdening subsequent downstream process (DSP) unit operations. Especially, DNA and endotoxins challenge purification operations due to their size and concentrations. Consequently, an early reduction in impurities will not only simplify the production process but also increase robustness while alleviating the workload afterward.

The lived experience of adolescent users of mental health services in Vienna, Austria: A qualitative study of personal recovery.

Problem: In Austria, one in four adolescents suffers from a mental health problem, yet there is a lack of adequate care structures. Therefore, the personal recovery of these adolescents is of particular interest. The aim of the study was to explore, from a Child and Adolescent Psychiatric Mental Health Nursing (CAPMHN) perspective, how adolescent users of mental health services in Vienna, Austria experienced personal recovery after a stay in hospital, and to discover what had influenced the personal recovery of adolescent users of mental health services in Vienna.