Tumor cell membrane-based vaccines: A potential boost for cancer immunotherapy.

Because therapeutic cancer vaccines can, in theory, eliminate tumor cells specifically with relatively low toxicity, they have long been considered for application in repressing cancer progression. Traditional cancer vaccines containing a single or a few discrete tumor epitopes have failed in the clinic, possibly due to challenges in epitope selection, target downregulation, cancer cell heterogeneity, tumor microenvironment immunosuppression, or a lack of vaccine immunogenicity. Whole cancer cell or cancer membrane vaccines, which provide a rich source of antigens, are emerging as viable alternatives.

Parallel mechanical computing: Metamaterials that can multitask.

Decades after being replaced with digital platforms, analogue computing has experienced a surging interest following developments in metamaterials and intricate fabrication techniques. Specifically, wave-based analogue computers which impart spatial transformations on an incident wavefront, commensurate with a desired mathematical operation, have gained traction owing to their ability to directly encode the input in its unprocessed form, bypassing analogue-to-digital conversion. While promising, these systems are inherently limited to single-task configurations.

SCRMshaw: Supervised cis-regulatory module prediction for insect genomes.

As the number of sequenced insect genomes continues to grow, there is a pressing need for rapid and accurate annotation of their regulatory component. SCRMshaw is a computational tool designed to predict cis-regulatory modules ("enhancers") in the genomes of various insect species. A key advantage of SCRMshaw is its accessibility.

Regulatory genome annotation of 33 insect species.

Annotation of newly sequenced genomes frequently includes genes, but rarely covers important non-coding genomic features such as the -regulatory modules-e.g., enhancers and silencers-that regulate gene expression.

Water insecurity is associated with intimate partner violence among female adolescents and youth but not males in rural Tanzania: A cross-sectional study.

Food insecurity increases intimate partner violence (IPV), but less is known about water insecurity (WI) and IPV. We examined the association between household WI and IPV among adolescents and youth in the Mbeya and Iringa regions of Tanzania. The cross-sectional sample comprised 977 males and females aged 18-23 years living in rural, impoverished households.

Mechanisms of transcriptional regulation in revealed by allele-specific expression.

Malaria control relies on insecticides targeting the mosquito vector, but this is increasingly compromised by insecticide resistance, which can be achieved by elevated expression of detoxifying enzymes that metabolize the insecticide. In diploid organisms, gene expression is regulated both in , by regulatory sequences on the same chromosome, and by acting factors, affecting both alleles equally. Differing levels of transcription can be caused by mutations in -regulatory modules (CRM), but few of these have been identified in mosquitoes.

Improved methodology for fixation and preparation of Aedes aegypti embryos.

The yellow fever mosquito Aedes aegypti is a major disease vector and an increasingly popular emerging model research organism. We present here an improved protocol for the collection, fixation, and preparation of A. aegypti embryos for immunohistochemical and in situ hybridization studies.

Normal-Tension Glaucoma Complicated by a Giant Internal Carotid-Ophthalmic Artery Aneurysm.

. We describe a patient with normal tension glaucoma (NTG) of several years whose management was complicated by the presence of a giant internal carotid-ophthalmic artery aneurysm. .

A new suite of reporter vectors and a novel landing site survey system to study cis-regulatory elements in diverse insect species.

Comparative analyses between traditional model organisms, such as the fruit fly Drosophila melanogaster, and more recent model organisms, such as the red flour beetle Tribolium castaneum, have provided a wealth of insight into conserved and diverged aspects of gene regulation. While the study of trans-regulatory components is relatively straightforward, the study of cis-regulatory elements (CREs, or enhancers) remains challenging outside of Drosophila. A central component of this challenge has been finding a core promoter suitable for enhancer-reporter assays in diverse insect species.

Conserved and novel enhancers in the Aedes aegypti single-minded locus recapitulate embryonic ventral midline gene expression.

Transcriptional cis-regulatory modules, e.g., enhancers, control the time and location of metazoan gene expression.

Perceptions and Prevalence of Cannabis Use in Women With Inflammatory Bowel Disease of Reproductive Age: A Cross-Sectional Study.

Background: Many patients with inflammatory bowel disease (IBD) may use cannabis for relief of symptoms. During pregnancy, however, cannabis exposure may be associated with adverse pregnancy outcomes. We aimed to determine the prevalence and perceptions of cannabis use in women with IBD.

"We Want to Eat and be Healthy just like Everybody Else:" How Social Infrastructures Affect Nutrition Equity in a Racialized Urban Community in the United States.

Background: Food security and nutrition equity, 2 social determinants of health, are impacted by the coronavirus disease 2019 (COVID-19) pandemic and the racialization of urban communities. Few studies to date have examined how the use of social infrastructures in the United States during COVID-19 affected the ability to achieve food security and nutrition equity.

Objectives: To describe how the use of social infrastructures impacts food security and nutrition equity in a majority Black and urban community in the United States.

Physical function trajectory after wrist or lower arm fracture in postmenopausal women: results from the Women's Health Initiative Study.

Unlabelled: Long-term physical functioning trajectories following distal forearm fracture are unknown. We found that women with versus those without distal forearm fracture were more likely to experience a 5-year decline in physical functioning, independent of initial physical functioning level. This association was most evident among women 80 years and older.

Development, validation, and transportability of several machine-learned, non-exercise-based VO prediction models for older adults.

Background: There exist few maximal oxygen uptake (VO) non-exercise-based prediction equations, fewer using machine learning (ML), and none specifically for older adults. Since direct measurement of VO is infeasible in large epidemiologic cohort studies, we sought to develop, validate, compare, and assess the transportability of several ML VO prediction algorithms.

Methods: The Baltimore Longitudinal Study of Aging (BLSA) participants with valid VO tests were included (n = 1080).

Economic vulnerabilities, mental health, and coping strategies among Tanzanian youth during COVID-19.

Background: The COVID-19 pandemic has exacerbated struggles for youth living in poor households. Youth in rural Tanzania are particularly vulnerable given widespread poverty, lack of formal sector employment opportunities, and health risks. We examine influences of the pandemic on economic insecurity and mental health and explore the coping strategies employed by youth and their households.

Genome-wide association identifies novel ROP risk loci in a multiethnic cohort.

We conducted a genome-wide association study (GWAS) in a multiethnic cohort of 920 at-risk infants for retinopathy of prematurity (ROP), a major cause of childhood blindness, identifying 1 locus at genome-wide significance level (p < 5×10) and 9 with significance of p < 5×10 for ROP ≥ stage 3. The most significant locus, rs2058019, reached genome-wide significance within the full multiethnic cohort (p = 4.96×10); Hispanic and European Ancestry infants driving the association.

Inquiring Hg NMR Parameters by Combining Ab Initio Molecular Dynamics and Relativistic NMR Calculations.

Ab initio molecular dynamics (AIMD) sampling followed by relativistic density functional theory (DFT) Hg NMR calculations were performed for Hg organometallic complexes in water, dimethyl sulfoxide, and chloroform. The spin-orbit coupling, a relativistic effect, is a key factor for predicting δ(Hg) and (Hg-C) accurately, in conjunction with a dynamic treatment of the systems. Good agreement between the theoretical and experimental results is reached by adopting implicit (based on a continuum model) and explicit (solvent molecules treated quantum mechanically) solvation models.

Effect of Preoperative Trabecular Meshwork Pigmentation and Other Eye Characteristics on Outcomes of Combined Phacoemulsification/Minimally Invasive Glaucoma Surgery.

Purpose: To investigate associations between pigmentation of the trabecular meshwork (PTM) and other preoperative eye characteristics and outcomes of minimally invasive glaucoma surgery combined with phacoemulsification (Phaco/MIGS).

Design: Retrospective interventional case series.

Participants: Academic glaucoma clinic patients with symptomatic cataract and glaucoma treated with combined Phaco/MIGS.

Mechanisms of transcriptional regulation in revealed by allele specific expression.

Malaria control relies on insecticides targeting the mosquito vector, but this is increasingly compromised by insecticide resistance, which can be achieved by elevated expression of detoxifying enzymes that metabolize the insecticide. In diploid organisms, gene expression is regulated both in , by regulatory sequences on the same chromosome, and by acting factors, affecting both alleles equally. Differing levels of transcription can be caused by mutations in -regulatory modules (CRM), but few of these have been identified in mosquitoes.

Improvement in Nasal Symptoms of Chronic Rhinitis after Cryoablation of the Posterior Nasal Nerve.

Objective: To determine the efficacy of posterior nasal nerve (PNN) cryoablation for improving the symptoms of chronic rhinitis.

Study Design: Retrospective cohort study.

Setting: A private practice.

Reassessing rumination: Validity of the Day Reconstruction Method for Rumination (DRM-R) to assess episodes of rumination in daily life.

Rumination is a robust vulnerability to depression and potential treatment target. However, we know relatively little about rumination in daily life. This study tested the validity of a new approach for assessing daily episodes of rumination, the Day Reconstruction Method for Rumination (DRM-R).

Screening for orthostatic hypotension in the geriatric population in a real-world primary care setting reduces prescribed antihypertensive medications.

Background: To determine if outpatient screening for orthostatic hypotension (OH) in the geriatric population results in fewer prescribed antihypertensive medications and if a relationship exists between OH and specific pharmacologic classes of antihypertensive medications.

Materials And Methods: Patients ≥ 65 years were screened for OH, defined as a decrease in systolic blood pressure (SBP) ≥ 20 mm Hg or a decrease in diastolic blood pressure (DBP) ≥ 10 mm Hg after standing for 3 minutes. Sitting blood pressure (BP) was measured after patients had been seated quietly in an exam room.