The effect of high-pressure jet processing on cocoa stability in chocolate milk.

Fat-free chocolate milk formulations containing skim milk, cocoa powder, and sugar were thermally treated and then processed using high-pressure jet (HPJ) technology from 125 to 500 MPa. The rheological properties and stability of HPJ-treated chocolate milks were compared with controls (no HPJ processing) prepared both with and without added κ-carrageenan. As expected, carrageenan-free chocolate milk exhibited immediate phase separation of the cocoa powder, whereas formulations containing κ-carrageenan were stable for 14 d.

Genetic parameters of calf morbidity and stayability for US organic Holstein calves.

The objectives of this study were to estimate genetic parameters of calf health in organic US Holstein calves. Calves were born on farms across the United States from 2006 to 2019. Three calf health traits were evaluated in the study: calf respiratory disease until 365 d of age, calf scours until 60 d of age, and heifer stayability until 365 d of age.

Rational Design of Nonbonded Point Charge Models for Divalent Metal Cations with Lennard-Jones 12-6 Potential.

Exploring a metal-involved biochemical process at a molecular level often requires a reliable description of metal properties in aqueous solution by classical nonbonded models. An additional term for considering ion-induced dipole interactions was previously proposed to supplement the widely used Lennard-Jones 12-6 potential (known as the 12-6-4 LJ-type model) with good accuracy. Here, we demonstrate an alternative to modeling divalent metal cations (M) with the traditional 12-6 LJ potential by developing nonbonded point charge models for use with 11 water models: TIP3P, SPC/E, SPC/E, TIP4P-Ew, TIP4P-D, and TIP4P/2005 and the more recent OPC3, TIP3P-FB, OPC, TIP4P-FB, and a99SB-.

Lactational performance, rumen fermentation, and enteric methane emission of dairy cows fed an amylase-enabled corn silage.

This study investigated the effects of an amylase-enabled corn silage on lactational performance, enteric CH emission, and rumen fermentation of lactating dairy cows. Following a 2-wk covariate period, 48 Holstein cows were blocked based on parity, days in milk, milk yield (MY), and CH emission. Cows were randomly assigned to 1 of 2 treatments in an 8-wk randomized complete block design experiment: (1) control corn silage (CON) from an isogenic corn without α-amylase trait and (2) Enogen hybrid corn (Syngenta Seeds LLC) harvested as silage (ECS) containing a bacterial transgene expressing α-amylase (i.

Relationship of daily total somatic cell output with somatic cell concentration and clinical mastitis.

Somatic cell count (SCC) measures the concentration of somatic cells in milk and is used as a mastitis diagnostic tool. It is plausible that variation in milk yield could alter the relationship between SCC and mastitis status. Our objective was to evaluate total daily SCC output as a predictor of clinical mastitis.

Histidine dose-response effects on lactational performance and plasma amino acid concentrations in lactating dairy cows: 2. Metabolizable protein-deficient diet.

The objective of this experiment was to determine the effect of increasing digestible His (dHis) levels with a rumen-protected (RP) His product on milk production, milk composition, and plasma AA concentrations in lactating dairy cows fed a metabolizable protein (MP)-deficient diet, according to the National Research Council dairy model from 2001. The companion paper presents results on the effect of increasing dHis dose with a MP-adequate basal diet. Twenty Holstein cows, of which 8 were rumen-cannulated, were used in a replicated 4 × 4 Latin square design experiment with four 28-d periods.

Histidine dose-response effects on lactational performance and plasma amino acid concentrations in lactating dairy cows: 1. Metabolizable protein-adequate diet.

The objective of this experiment was to determine the effect of increasing digestible His (dHis) doses on milk production, milk composition, and plasma AA concentrations in lactating dairy cows fed diets that meet or exceed their energy and metabolizable protein (MP) requirements. In a companion paper (Räisänen et al., 2021) results are presented on the effect of increasing dHis dose with an MP-deficient basal diet.

Stability of milk fatty acid profile during simulated shipping for analysis by gas chromatography.

Milk fat 10 C18:1 can be used in diagnosing low milk fat production on dairy farms because it is a specific marker of biohydrogenation-induced milk fat depression. Individual fatty acids (FA), including the C18:1 isomer, can be determined only by gas-liquid chromatography. The analysis is currently available at a limited number of laboratories and often requires long-distance shipping.

Determination of relationships between rumination and milk fat concentration and fatty acid profile using data from commercial rumination sensing systems.

Milk fat production is highly influenced by nutrition and rumen fermentation. Rumination is an essential part of the ruminant digestive process and can serve as an indicator of rumen fermentation. The objective of this research was to quantify variation in rumination time between and within dairy herds and test for relationships between rumination time and milk fat production and fatty acid (FA) profile as a proxy of rumen fermentation.

Temporal changes in total and metabolically active ruminal methanogens in dairy cows supplemented with 3-nitrooxypropanol.

The purpose of this study was to investigate the effect of 3-nitrooxypropanol (3-NOP), a potent methane inhibitor, on total and metabolically active methanogens in the rumen of dairy cows over the course of the day and over a 12-wk period. Rumen contents of 8 ruminally cannulated early-lactation dairy cows were sampled at 2, 6, and 10 h after feeding during wk 4, 8, and 12 of a randomized complete block design experiment in which 3-NOP was fed at 60 mg/kg of feed dry matter. Cows (4 fed the control and 4 fed the 3-NOP diet) were blocked based on their previous lactation milk yield or predicted milk yield.

Limiting total mixed ration availability alters eating and rumination patterns of lactating dairy cows.

The objective of this study was to investigate the effect of restricting total mixed ration (TMR) eating time with or without supplemental long hay offered on diurnal total intake, rumination, and rumen pH patterns in mid-lactation dairy cows. Eight multiparous Holstein cows were used in a replicated 4 × 4 Latin square design study with 21-d periods. The basal diet was the same for all 4 treatments with or without additional long grass hay (the same hay used in the TMR).

Comparison of the effects of short-term feeding of sodium acetate and sodium bicarbonate on milk fat production.

Supplementation with sodium acetate (NaAcet) increases milk fat production through an apparent stimulation of de novo lipogenesis in the mammary gland. Sodium acetate increases acetate supply to the mammary gland, but it also increases dietary cation-anion difference, which can also increase milk fat yield. The objective of this study was to determine if the effect of NaAcet on milk fat production was due to an increase in acetate supply or an increase in dietary cation-anion difference.

Effects of fat supplements containing different levels of palmitic and stearic acid on milk production and fatty acid digestibility in lactating dairy cows.

Fat supplements based on palmitic acid (PA) or stearic acid (SA) are expected to have different effects on milk production and nutrient metabolism in lactating dairy cows. In this study, the effects of prilled fat supplements containing different levels of PA and SA were tested in 12 high-producing multiparous cows (pretrial milk yield = 53.4 ± 8.

Starch-protein interaction in the rumen of weaned dairy calves.

The objectives of this study were to investigate the effect of starch and protein interaction on rumen environment, in situ digestion, and total-tract digestibility of nutrients in weaned dairy calves between 8 and 16 wk of age. Sixteen rumen-cannulated calves were randomly divided into 4 dietary treatment groups with 4 calves fed in each treatment. The treatment diets had 2 levels of starch [18%, low starch (LS), or 38%, high starch (HS)] and 2 levels of protein [16%, low protein (LP), or 22%, high protein (HP)] on a dry matter (DM) basis in calf grower: (1) LPLS, (2) LPHS, (3) HPLS, and (4) HPHS.

Genetic parameters for stayability of Holsteins in US organic herds.

The objectives of this study were to estimate genetic parameters for stayability in US organic Holstein dairy cows and estimate genetic correlations with nationally evaluated traits of interest. Stayability is the binary trait for success or failure to remain in the herd until a given time point. We used birth, calving, and cull dates from 16 USDA certified organic farms recommended by industry personnel as herds maintaining individual cow records and using artificial insemination.

Effects of the macroalga Asparagopsis taxiformis and oregano leaves on methane emission, rumen fermentation, and lactational performance of dairy cows.

Asparagopsis taxiformis (AT) is a source of multiple halogenated compounds and, in a limited number of studies, has been shown to decrease enteric CH emission in vitro and in vivo. Similarly, oregano has been suggested as a potential CH mitigating agent. This study consisted of 2 in vitro and 2 in vivo experiments.

Freezing kinetics and microstructure of ice cream from high-pressure-jet processing of ice cream mix.

The effect of high-pressure-jet (HPJ) processing (0-500 MPa) on low-fat (6% fat) ice cream was studied by evaluating physiochemical properties before freezing, during dynamic freezing, and after hardening. An HPJ treatment ≥400 MPa decreased the density, increased the apparent size of colloidal particles, and altered rheological behavior (increased non-Newtonian behavior and consistency coefficients) of low-fat ice cream mix before freezing. During dynamic freezing, the particle size and consistency coefficient decreased but remained higher in 400 MPa-treated samples vs.

Lactational performance, enteric gas emissions, and plasma amino acid profile of dairy cows fed diets with soybean or canola meals included on an equal protein basis.

This study investigated the effects of feeding solvent-extracted canola meal (CM), extruded soybean meal (ESBM), or solvent-extracted soybean meal (SSBM) on an equivalent crude protein basis on performance, plasma AA profiles, enteric gas emissions, milk fatty acids, and nutrient digestibility in lactating dairy cows. Fifteen Holstein cows (95 ± 20 d in milk) were used in a replicated 3 × 3 Latin square design experiment with 3 periods of 28 d each. Treatments were 3 diets containing 17.

Genetic parameters of passive transfer of immunity for US organic Holstein calves.

Passive transfer of immunity is important for calf health and survival. The objectives of this study were to estimate genetic parameters for calf passive transfer of immunity through producer-recorded serum total protein (STP) and to determine associations with other routinely evaluated traits in organic Holstein calves (n = 16,725) that were born between July 2013 to June 2018; a restricted subset (n = 7,518) of calves with known Holstein maternal grandsires was analyzed separately. Producers measured STP on farm, and STP was extracted from farm management software.

Replacing soybean hulls with grass hay on growth, intake, total tract digestibility, and rumen microbial nitrogen production of weaned Holstein dairy calves from 8 to 16 weeks of age.

The objective of this experiment was to determine the effects of replacing soybean hull (SBH) pellets with grass hay (GH) on growth, intake, total-tract digestibility, and rumen microbial nitrogen production of weaned dairy calves from 8 to 16 wk of age. Holstein calves (n = 16) were randomly assigned to 1 of 4 rations containing, on a dry matter (DM) basis, 76% grower with the remaining 24% being made up of different amounts of SBH pellets and GH [0% GH, 24% SBH (0GH); 8% GH, 16% SBH (8GH); 16% GH, 8% SBH (16GH); and 24% GH, 0% SBH (24GH)]. Calves were weaned at 6 wk, housed individually, and studied from 8 to 16 wk of age.

Relationship of body weight at first calving with milk yield and herd life.

The objectives of this study were to investigate the association of body weight (BW) at first calving (BWFC) and maturity rate (MR; BWFC as a percentage of mature BW) with first-lactation 305-d milk yield (FLMY), milk yield (MY) in the 24 mo following first calving (24MMY), herd life, and BW change (BWC) through the first month of lactation in Holstein heifers. We retrieved daily milk production records and daily BW records from AfiFarm (S. A.

Enteric methane emission, milk production, and composition of dairy cows fed 3-nitrooxypropanol.

This study examined the effect of 3-nitrooxypropanol (3-NOP), an investigational substance, on enteric methane emission, milk production, and composition in Holstein dairy cows. Following a 3-wk covariate period, 48 multi- and primiparous cows averaging (± standard deviation) 118 ± 28 d in milk, 43.4 ± 8 kg/d milk yield, and 594 ± 57 kg of body weight were blocked based on days in milk, milk yield, and enteric methane emission and randomly assigned to 1 of 2 treatment groups: (1) control, no 3-NOP, and (2) 3-NOP applied at 60 mg/kg feed dry matter.

MILK Symposium review: Improving the productivity, quality, and safety of milk in Rwanda and Nepal.

Dairy production plays an important role in the lives of many people in Rwanda and Nepal. The aim of the Feed the Future Innovation Lab for Livestock Systems (LSIL; Gainesville, FL) is to introduce new location-appropriate technologies and to improve management practices, skills, knowledge, capacity, and access to inputs across livestock value chains in developing countries such as Rwanda and Nepal. To assist LSIL, our first aim was to describe gaps in the management of cows and in milk processing that constrain milk quality and quantity in Rwanda and Nepal.

Short communication: Short-term effect of 3-nitrooxypropanol on feed dry matter intake in lactating dairy cows.

The objective of this study was to investigate the effect of 3-nitrooxypropanol (3-NOP), an enteric methane inhibitor under investigation, on short-term dry matter intake (DMI) in lactating dairy cows. Following a 1-wk adaptation period, 12 multiparous Holstein cows were fed a basal total mixed ration (TMR) containing increasing levels of 3-NOP during 5 consecutive, 6-d periods. The experiment was conducted in a tiestall barn.

Meta-analysis of the relationship between milk trans-10 C18:1, milk fatty acids <16 C, and milk fat production.

The economic value of milk fat and its responsiveness to management strategies provides strong interest in maximizing milk fat production by minimizing occurrence of biohydrogenation-induced milk fat depression (BH-MFD) and maximizing de novo synthesized fatty acids (FA). Tools that allow a timely diagnosis of BH-MFD would improve nutritional management. Specific milk FA or FA categories correlate to milk fat concentration and are of interest for diagnosing the cause of changes in milk fat concentration.