Extracellular Streptomyces vesicles: amphorae for survival and defence.

Blue-pigmented exudates arise as droplets on sporulated lawns of Streptomyces coelicolor M110 grown on agar plates. Our electron microscopical and biochemical studies suggest that droplets contain densely packed vesicles with large assemblies of different protein types and/or the polyketide antibiotic actinorhodin. Frozen-hydrated vesicles were unilamellar with a typical bilayer membrane, and ranged from 80 to 400 nm in diameter with a preferred width of 150-300 nm.

Independent encoding of grating motion across stationary feature maps in primary visual cortex visualized with voltage-sensitive dye imaging.

In early visual cortex different stimulus parameters are represented in overlaid feature maps. Such functioning was extensively explored by the use of drifting gratings characterized by orientation, spatial-temporal frequency, and direction of motion. However surprisingly, the direct cortical visuotopic drift of the gratings' stripy pattern has never been detected simultaneously to these stationary feature maps.

A selective assay to detect chitin and biologically active nano-machineries for chitin-biosynthesis with their intrinsic chitin-synthase molecules.

A new assay system for chitin has been developed. It comprises the chitin-binding protein ChbB in fusion with a His-tag as well as with a Strep-tag, the latter of which was chemically coupled to horseradish peroxidase. With the resulting complex, minimal quantities of chitin are photometrically detectable.

Assessing persistence and long-range transport potential of current-use pesticides.

Despite the fact that current-use pesticides (CUP) have different chemical properties to first-generation organochlorine products, the long-term and long-range environmental behavior of these chemicals is still unclear. Data for 45 active ingredients of CUPs were collected, most of which originate from the results of simulation tests submitted for authorization. According to the Stockholm Convention on persistent organic pollutants (POPs), two of the 45 CUPs exceed both screening level criteria for persistence and long-range transport potential (LRTP).

Microbial rhodopsins: scaffolds for ion pumps, channels, and sensors.

Microbial rhodopsins have been intensively researched for the last three decades. Since the discovery of bacteriorhodopsin, the scope of microbial rhodopsins has been considerably extended, not only in view of the large number of family members, but also their functional properties as pumps, sensors, and channels. In this review, we give a short overview of old and newly discovered microbial rhodopsins, the mechanism of signal transfer and ion transfer, and we discuss structural and mechanistic aspects of phototaxis.

Symbols as self-emergent entities in an optimization process of feature extraction and predictions.

In the mammalian cortex the early sensory processing can be characterized as feature extraction resulting in local and analogue low-level representations. As a direct consequence, these map directly to the environment, but interpretation under natural conditions is ambiguous. In contrast, high-level representations for cognitive processing, e.

Structural and functional features of the Escherichia coli F1-ATPase.

The structural organization and overall dimensions of the Escherichia coli F1-ATPase in solution has been analyzed by synchroton X-ray scattering. Using an independent ab initio approach, the low-resolution shape of the hydrated enzyme was determined at 3.2 nm resolution.

Evidence for toxic effects of alkylphenols from Ginkgo biloba in the hen's egg test (HET).

Extracts from the leaves of the Gingko tree (Ginkgo biloba L.) are therapeutically used for the treatment of peripheral and cerebral vascular disorders as well as multi-infarct or Alzheimer-type dementia. As constituents with potential contact allergenic and toxic properties in crude Ginkgo extracts a group of alkylphenols (e.

Inactivation of fusidic acid by resistant Streptomyces strains.

Streptomyces lividans and several other Streptomyces species are resistant to the steroid-like antibiotic fusidic acid. This resistance is mediated by structural modification of the antibiotic. Using TLC, CD, UV, IR, NMR and mass spectroscopy the structure of one of the resulting inactive compounds was determined.