Serotyping and antimicrobial resistance of Actinobacillus pleuropneumoniae isolates from fattening pigs in Poland from 2019 to 2024.

Background: Actinobacillus pleuropneumoniae is a prevalent respiratory pathogen causing substantial economic losses in swine production worldwide. The bacterium's ability to rapidly develop antimicrobial resistance (AMR) poses a significant challenge to effective treatment and control. In Poland, limited data on A.

First molecular detection of hemotropic Mycoplasma spp. and molecular screening of other vector-borne pathogens in camels from the greater Cairo metropolitan area, Egypt.

In Egypt, knowledge about vector-borne bacterial pathogens in camels remains limited. To address this gap, 181 blood samples from adult one-humped camels (Camelus dromedarius) in the greater Cairo metropolitan area were collected from October 2021 to March 2022. Through PCR assays, four pathogens were detected, where Anaplasmataceae being the most common (54.

Impact of pollution on microbiological dynamics in the pistil stigmas of Orobanche lutea flowers (Orobanchaceae).

Our understanding of the basic relationships of microbiota associated with flowers is still quite limited, especially regarding parasitic plant species. The transient nature of flower parts such as pistil stigmas provides a unique opportunity for temporal investigations. This is the first report of the analysis of bacterial and fungal communities associated with the pistil stigmas of the lucerne parasite, Orobanche lutea.

Morphometric assessment of mandibles with complications resulting from temporomandibular joint ankylosis in children from 4 months to 3 years of age.

: The main aim of this paper was to perform the morphological assessment of children's mandibles of different etiology of dys-functions within the temporomandibular joint, from isolated idiopathic ankylosis to craniofacial malformations co-existing with genetic disorders. : The investigations encompassed seven patients at the age of 0-3. Measurements were conducted on the basis of data obtained from computed tomography.

Evaluation of Bioactive Compounds and Chemical Elements in Herbs: Effectiveness of Choline Chloride-Based Deep Eutectic Solvents in Ultrasound-Assisted Extraction.

In this study, the effectiveness of three choline chloride (ChCl)-based deep eutectic solvents (DESs) formed using malonic acid (MalA), glycerol (Gly), and glucose (Glu) as hydrogen bond donors and two conventional solvents (50% methanol and 50% ethanol) for ultrasonic-assisted extraction (UAE) of antioxidant compounds from four herbs (chamomile, lemon balm, nettle, and spearmint) were estimated. The antioxidant capacity (AC) of the obtained herb extracts was determined by the modified 2,2'-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) (ABTS), 2,2-diphenyl-1-picrylhydrazyl (DPPH), and cupric reducing antioxidant capacity (CUPRAC) methods. Profiles of phenolic acids, flavonoid aglycones, and flavonoid glycosides in the green and conventional herb extracts were quantitatively analyzed using ultra-performance liquid chromatography (UPLC).

Influence of Plant Additives on Changes in the Composition of Fatty Acids, Lipid Quality Indices and Minerals of Fermented Dairy Products from Cow's Milk.

The aim of this study was to assess the effect of selected plant additives on changes in the content of fatty acids, lipid quality indicators and mineral composition of yogurts produced from cow's milk. The analysis included natural yogurts and yogurts enriched with 10% of chia seeds, hulled hemp seeds, quinoa seeds and oat bran. The fatty acid composition, the content of lipid quality indicators and the content of mineral components was varied in all analyzed yogurts.

Prediction of Seven Artificial Intelligence-Based Intraocular Lens Power Calculation Formulas in Medium-Long Caucasian Eyes.

To compare the accuracy of seven artificial intelligence (AI)-based intraocular lens (IOL) power calculation formulas in medium-long Caucasian eyes regarding the root-mean-square absolute error (RMSAE), the median absolute error (MedAE) and the percentage of eyes with a prediction error (PE) within ±0.5 D. Data on Caucasian patients who underwent uneventful phacoemulsification between May 2018 and September 2023 in MW-Med Eye Center, Krakow, Poland and Kyiv Clinical Ophthalmology Hospital Eye Microsurgery Center, Kyiv, Ukraine were reviewed.

Vascular Cell Adhesion Molecule 1 and E-Selectin as Potential Cardiovascular Risk Biomarkers in Psoriasis.

Vascular cell adhesion molecule-1 (VCAM-1) and E-selectin are involved in different inflammatory diseases and may be potential cardiovascular risk biomarkers in psoriasis. They play an important role in regulating the recruitment and adhesion to endothelial cells during inflammation, affecting various conditions like vasculitis, atherosclerosis, and cardiovascular diseases. Positive outcomes have been observed when using Tumor Necrosis Factor Alpha (TNF-α) inhibitors and biological therapies that target selectins to control the functioning of endothelial cells and reduce inflammation in psoriasis and related conditions.

The Impact of Nanoparticles and Molecular Forms of TiO on the Rhizosphere of Plants in the Example of Common Wheat ( L.)-Shifts in Microbiome Structure and Predicted Microbial Metabolic Functions.

This study investigated the effects of various titanium nanoparticles (TiONPs) on the structure, function, and trophic levels of the wheat rhizobiome. In contrast to the typically toxic effects of small nanoparticles (~10 nm), this research focused on molecular TiO and larger nanoparticles, as follows: medium-sized (68 nm, NPs1) and large (>100 nm, NPs2). The results demonstrated significant yet diverse impacts of different TiO forms on the rhizosphere microbiota.

Chromosomal rDNA Distribution Patterns in Clonal Triploid Hybrids (Teleostei, Cobitidae): Insights into Parental Genomic Contributions.

Interspecific hybridization between relative species (with a diploid genome designated as TT), (EE) and (NN) and the successive polyploidization with transitions from sexuality to asexuality experienced by triploid hybrids likely influence their chromosomal rearrangements, including rearrangements of ribosomal DNA (rDNA) distribution patterns. Previously, we documented distinct karyotypic differences: exhibited bi-armed chromosomes while showed uni-armed chromosomes with rDNA-positive hybridization signals, respectively. In this study, fluorescence in situ hybridization (FISH) with rDNA and rDNA probes was used to analyze and compare chromosomal distribution patterns of rDNAs in clonally reproduced triploid hybrids of different genomic constitutions ETT, ETN, EEN and EET (referred to using acronyms denoting the haploid genomes of their parent species), and their parental species.

Effects of Standardized Natural Citrus Extract on Growth, Gut Health, Carcass Quality, and Welfare of Broiler Chickens.

This study aimed to investigate the effects of a Standardized Natural Citrus Extract (SNCE) on broiler chickens' growth performance, gut health, carcass quality, and welfare. A total of 756 one-day-old Ross 308 males were randomly assigned to two groups: a control group (CTL) fed with a standard diet, and a citrus group (SNCE) fed with the same standard diet supplemented with 250 g/ton of feed of SNCE. Growth performance was recorded weekly until d 35, while mortality was recorded daily.

Urogenital Cancer Epidemiology in Poland (1980-2020): A Narrative Review.

Despite advances in prophylaxis, early diagnosis, and treatment, urogenital cancers represent a significant challenge to public health in Poland due to their relatively high prevalence and mortality rates. This narrative review aims to explore contemporary evidence on the epidemiology of urogenital cancers in Poland, such as prostate cancer, bladder cancer, kidney cancer, testicular cancer, and penile cancer, focusing on current and historical status and trends in the broader context of healthcare delivery. The literature consistently indicates that urogenital cancer continues to be a significant contributor to cancer incidence and mortality rates in Poland.

The Perception of Organic Food Characteristics and the Demographic and Social Profile of Consumers: A Study of the Polish Market.

The aim of the research was to determine the relationship between the perception of organic food characteristics and the demographic and social profile of consumers on the Polish market. The research focused on the general characteristics and features of plant and animal products offered on the organic food market compared to conventional food. The study was conducted on a sample of 1020 respondents from different regions of Poland using structural equation modelling, which allowed for the assessment of regression and covariance relationships between variables.

Effects of Acorn Flour Addition on Baking Characteristics of Wheat Flour.

This study aimed to evaluate the impact of incorporating acorn flour (at levels ranging from 5% to 50%) on the baking properties of wheat flour (type 750). The assessment focused on key baking parameters, including fermentation properties, pasting behavior, and dough rheological characteristics such as farinographic and extensographic properties. A laboratory baking test was conducted to compare the technological properties of wheat and wheat-acorn breads, assessing dough and bread yields, oven and total losses, bread volume, and crumb hardness.

Moisture Loss from Cheese During Baking: Influence of Cheese Type, Cheese Mass, and Temperature.

This study examined how temperature, cheese mass and moisture content impact moisture loss rate in various cheeses during baking. Understanding these factors is essential for determining the browning properties of cheese during baking. Eight cheese types, differing in moisture content, were baked at 100-200 °C in a halogen moisture analyzer, and moisture loss over time was recorded.

The First Complete Chloroplast Genome Sequence of subsp. Stapf (), the Putative Ancestor of the Genus .

ssp. (synonym ), a putative ancestor of the genus , has been classified within , although recent phylogenetic studies suggest that it represents a distinct species. This study reports the first complete chloroplast genome of , highlighting its structure, genetic composition, and phylogenetic relationships within and related Triticiceae species.

Global dissemination of Klebsiella pneumoniae in surface waters: Genomic insights into drug resistance, virulence, and clinical relevance.

The aquatic environment is a major pathway for the spread of antibiotic resistance (AR) among microorganisms. Among these, Klebsiella pneumoniae reveals high genome plasticity, adaptability, and the ability to colonize humans, animals, and the natural environment, awarding it a significant role in the spread of AR. This work presents an in-depth analysis of the whole sequences of 149 K.

Mycophenolate mofetil: an update on its mechanism of action and effect on lymphoid tissue.

Introduction: Mycophenolate mofetil (MMF) is an immunosuppressive drug administered in the management of both autoimmune diseases and organ transplantation. The main aims of the study were: (a) to obtain information regarding the safety of using MMF in respect of its effect on normal T and B cells in lymphoid tissues; (b) to investigate whether the generation of inducible Foxp3-expressing regulatory T cells (Treg) might constitute additional mechanisms underlying the immunosuppressive properties of MMF.

Methods: The effect of MMF ( studies) and its active metabolite, mycophenolic acid, ( studies) on murine CD4 and CD8 T cells as well as B cells was determined, regarding: (a) absolute count, proliferation and apoptosis of these cells ( studies); (b) absolute count of these cells in the head and neck lymph nodes, mesenteric lymph nodes and the spleen ( studies).

Lipopolysaccharide affects metabolic processes and energy homeostasis in the corpus luteum.

Introduction: Chronic inflammation caused by infections has a significant negative impact on the reproductive system and impairs fertility. The corpus luteum (CL) plays a central role not only in regulating the ovary cycle, but also in implantation of the embryo and maintenance of early pregnancy through the secretion of progesterone. Understanding the intricate interplay between inflammatory processes and reproductive organ's function is crucial for the development of effective therapeutic strategies to alleviate reproductive disorders and improve fertility.

Investigation of genes expression of the JAK/STAT signalling pathway and AMPs in the presence of Borrelia spirochetes in Ixodes ricinus.

Multicellular animals need to control the spread of invading pathogens. This is a particular challenge for blood-feeding vectors such as ticks, which ingest large amounts of blood potentially laden with harmful microorganisms. Ticks have a basic innate immune system and protect themselves from infection through innate immune responses involving pathways such as Janus kinase (JAK) or the signalling transducer activator of transcription (STAT).

Biosynthetic pathway for leukotrienes is stimulated by lipopolysaccharide and cytokines in pig endometrial stromal cells.

An inflammatory response is related to different inflammatory mediators generated by immune and endometrial cells. The links between lipopolysaccharide (LPS), cytokines, and leukotrienes (LTs) in endometrial stromal cells remain unclear. This study aimed to examine the influence of LPS, tumor necrosis factor (TNF)-α, interleukin (IL)-1β, IL-4 and IL-10 on 5-lipooxygenase (5-LO), LTA4 hydrolase (LTAH) and LTC4 synthase (LTCS) mRNA and protein abundances, and LTB4 and cysteinyl (cys)-LTs release including LTC4, by the cultured pig endometrial stromal cells, as well as on cell viability.

Anthropometric but not motor characteristics of young volleyball players were improved after a one-week-long intense training sports camp.

This study aimed to examine potential changes in the anthropometric and motor characteristics of volleyball players aged 17.98 ± 0.51 years after participation in a week-long sports camp.

Pulmonary function in swimmers exposed to disinfection by-products: a narrative review.

Swimming produces many psychophysiological effects, including blood, hormonal, enzymatic, pulmonary, cardiovascular and energetic adaptations. However, asthma and allergies are becoming increasingly prevalent medical issues among elite endurance-trained swimmers, where exercise-induced asthma or bronchospasm is frequently reported. Heavy endurance swimming training, especially under adverse conditions, stresses the airway mucosa, leading to inflammatory changes, as observed in induced sputum in competitive swimmers.

An assessment of physiological and health responses in Catla catla fingerlings after polystyrene microplastic exposure.

Microplastics (MPs) form when plastic debris is released into the aquatic environment, where they decompose and have deleterious effects on aquatic life. This study aimed to examine the harmful impacts of polystyrene MPs (PS-MPs) on the growth, carcass composition, hematology, digestibility, histopathology, and mineral analysis of Catla catla (11.09 ± 0.