Revolutionizing Tuberculosis Management With Clustered Regularly Interspaced Short Palindromic Repeats (CRISPR)-Cas Technology: A Comprehensive Literature Review.

Clustered regularly interspaced short palindromic repeats (CRISPR)-Cas systems have gained attention for their revolutionary potential in tuberculosis (TB) management, providing a novel approach to both diagnostics and treatment. This technology, renowned for its ability to accurately target and modify genetic material, offers a promising solution to the limitations of current TB diagnostic methods, which often rely on time-consuming culture techniques or polymerase chain reaction (PCR)-based assays. One of the key advantages of CRISPR-Cas systems is their high specificity and sensitivity, making them well-suited for detecting , even in low-bacterial-load samples.

Relationship between homocysteine, vitamin B9, vitamin B12 levels methylenetetrahydrofolate reductase (C677T, A1298C) polymorphisms, and cryptogenic stroke in Tunisian adults´ patients: a case-control study.

Introduction: the relationship between elevated plasma homocysteine (Hcy) and stroke has been established, but this association remains indistinct in cryptogenic stroke in adults. Our aim is to investigate the association between homocysteine, vitamins B9 and B12, and cryptogenic stroke. Furthermore, to determine the correlation between methylenetetrahydrofolate reductase (MTHFR) polymorphism and biochemical levels in plasma.

Protective effects of Juglans regia oil on lead acetate-induced reprotoxicity in rats: an antioxidant, histological and computational molecular study.

Background: Lead (Pb) is a metal that affects many body systems, primarily the male reproductive system. This study aimed to examine the phytochemical profiling and beneficial effects of Juglans regia oil (JRO) in alleviating lead-induced reprotoxicity-associated oxidative injury in rats. Adult male Wistar rats were randomly divided into four groups as follows: control group received no treatment, Pb group was exposed to 0.

Laparoscopic Appendectomy in Children: Tunisian Experience.

Objective: The purpose of this study is to compare the surgical outcomes of open appendectomy (OA) and laparoscopic appendectomy (LA) for pediatric acute appendicitis in order to guide its future management.

Materials And Methods: We conducted a retrospective study including all children under the age of 14 years, who underwent appendectomy for acute appendicitis, during a period of 6 years (2013-2018). We divided our patients in two groups based on the surgical modality: the laparoscopic and open group.

CsZnCl: a lead-free all-inorganic perovskite with a large dielectric permittivity.

In recent years, inorganic perovskite materials based on metallic halides have attracted significant attention due to their non-toxicity and ease of synthesis, making them suitable for various applications. This article describes the slow evaporation approach at room temperature for the fabrication of a non-toxic inorganic perovskite based on metallic halide CsZnCl. This compound crystallizes in the orthorhombic phase of the space group, as confirmed by room temperature X-ray diffraction.

The effect of therapeutic education program on hypertensive Tunisian patients' knowledge: a randomized controlled trial.

Background: Hypertension is a prevalent non-communicable disease and unequivocally one of the most serious health threats of the twenty-first century. The prevention of both immediate and long-term consequences depends on ongoing therapeutic education.

Aims: To assess Tunisian hypertensive patients' knowledge of hypertension and then evaluate the impact of an educational program on knowledge among this population.

Self-Medication with Herbal Medicine Among Young Adult Cancer Patients: A Prospective Monocentric Study.

The aim of this study was to determine the incidence of the self-use of plants in cancer patients and its impact on overall survival (OS). This was a prospective study including young adult patients collected between January 15, 2018 and January 15, 2019 in the department of medical oncology at the Habib Bourguiba University Hospital. All patients were questioned about the concept of taking herbs.

Investigation of variable range hopping and dielectric relaxation in GdCrO orthochromite perovskites.

This study investigates the structural, electrical, and dielectric properties of GdCrO (GCO) compounds. X-ray diffraction analysis confirmed the formation of the perovskite phase of GCO, crystallizing with the space group. Scanning electron microscopy (SEM) was employed to examine the morphology and chemical composition of the powder, ensuring compound homogeneity, while transmission electron microscopy (TEM) provided insights into the internal structure and finer morphology of the GCO sample.

Improving arterial stiffness prediction with machine learning utilizing hemodynamics and biomechanical features derived from phase contrast magnetic resonance imaging.

Arterial stiffness has emerged as a prominent marker of risk for cardiovascular diseases. Few studies are interested in predicting symptomatic or asymptomatic arterial stiffness from hemodynamics and biomechanics parameters. Machine learning models can be used as an intelligent tool for arterial stiffness detection based on hemodynamic and biomechanical parameters.

Betalains from peels: UPLC-MS/MS metabolites profiling, computational investigation, and potential applicability as a raw meat colorant.

Given consumers', environmental and sustainability apprehensions, the meat industry investigated the natural colorant resources. As proof, betalain, peels (OSP) pigment, is premeditated in the meat industry. Here, OSP betalains were qualitatively profiled using UPLC-MS/MS, and 7 metabolites were identified: 6 betacyanins and a betaxanthin (arginine-betaxanthin).

New experimental methodology for determining the second crossover energy in insulators under stationary e-irradiation in a SEM.

A new experimental methodology is proposed which uses the electrostatic influence method (EIM) in scanning electron microscope (SEM) in order to estimate the second crossover energy E for uncharged insulators. This experimental methodology based on simultaneous time measurement of the displacement and leakage currents, is approached to the short pulse irradiation technique but under stationary e-irradiation and allows determining the intrinsic secondary electron emission yield, σ (σ is the value of the total secondary electron yield just at the beginning of the irradiation before significant charge accumulation or before the formation of a surface potential). The obtained value of E for soda-lime glass is confirmed by two additional experiments based on secondary electron imaging.

The quality of psychosocial health and psychosocial illness among women with and without shelter homes: a cross-sectional study.

Background: Existing research on the psychosocial wellbeing of sheltered women is primarily from Western contexts, with limited studies from collectivistic cultures like Pakistan. This study aims to compare the psychosocial health and illness of sheltered women in Pakistan with those of women living with their families.

Methods: A total of 184 sheltered women and 207 women from the general population from four Pakistani cities participated in this study.

Deep-GB: A novel deep learning model for globular protein prediction using CNN-BiLSTM architecture and enhanced PSSM with trisection strategy.

Globular proteins (GPs) play vital roles in a wide range of biological processes, encompassing enzymatic catalysis and immune responses. Enzymes, among these globular proteins, facilitate biochemical reactions, while others, such as haemoglobin, contribute to essential physiological functions such as oxygen transport. Given the importance of these considerations, accurately identifying Globular proteins is essential.

Graph-based machine learning model for weight prediction in protein-protein networks.

Proteins interact with each other in complex ways to perform significant biological functions. These interactions, known as protein-protein interactions (PPIs), can be depicted as a graph where proteins are nodes and their interactions are edges. The development of high-throughput experimental technologies allows for the generation of numerous data which permits increasing the sophistication of PPI models.

Impact of missense mutations on the structure-function relationship of human succinyl-CoA synthetase using in silico analysis.

The encephalomyopathic mtDNA depletion syndrome with methylmalonic aciduria is associated with succinyl-CoA synthetase (SCS) deficiency caused by pathogenic variants in genes encoding its two subunits. SCS is a mitochondrial enzyme involved in several metabolic pathways and acts as a heterodimer composed of α and β subunits encoded by SUCLG1 and SUCLA2 genes, respectively. The purpose of this study was to analyze the effects of the most pathogenic non-synonymous single nucleotide polymorphisms (nsSNPs) by applying, using different prediction tools, a filtering strategy, on the 343 and 365 nsSNPs found in SUCLG1 and SUCLA2 genes, respectively, retrieved from the databases, then to evaluate their structural and functional effects using homology modeling and molecular docking.

The latest research progress on polysaccharides-based biosensors for food packaging: A review.

In recent years, polysaccharide-based biosensors have emerged as promising technologies for intelligent food packaging, offering innovative solutions to enhance food quality and safety. This review highlights advancements in designing, developing, and applying these biosensors, particularly those utilizing polysaccharides such as chitosan, cellulose and alginate. Engineered with nanomaterials like ZnO, silver, and carbon nano-tubes demonstrated high sensitivity in real-time monitoring of food spoilage indicators, including pH changes, volatile nitrogen compounds and microbial activity.