International consensus statement on microbiome testing in clinical practice.

There is growing interest in the potential exploitation of the gut microbiome as a diagnostic tool in medicine, but evidence supporting its clinical usefulness is scarce. An increasing number of commercial providers offer direct-to-consumer microbiome diagnostic tests without any consensus on their regulation or any proven value in clinical practice, which could result in considerable waste of individual and health-care resources and potential drawbacks in the clinical management of patients. We convened an international multidisciplinary expert panel to standardise best practices of microbiome testing for clinical implementation, including recommendations on general principles and minimum requirements for their provision, indications, pre-testing protocols, method of analyses, reporting of results, and potential clinical value.

Assessment of the Linnaean type material of the Nose-horned viper, Vipera ammodytes (Linnaeus, 1758).

Within the framework of surveys investigating the origins of Linnaean taxa, we assessed the evidence supporting historical taxonomic and nomenclatural proposals and decisions regarding the nose-horned viper (Vipera ammodytes). The viper was described as Coluber ammodytes Linnaeus, 1758 from "the Orient." Bruno (1968) assessed the pre-Systema Naturae Ed.

Redescription of the poorly known Allopodoiulus schioedtei (Verhoeff, 1898) and the identity of Xestoiulus luteus (Attems, 1951) (Diplopoda: Julida: Julidae).

Based on old type material and freshly collected specimens, we evaluate and clarify the taxonomic status of two poorly known Balkan species, Allopodoiulus schioedtei (Verhoeff, 1898) and Xestoiulus luteus (Attems, 1951), and propose their synonymy with X. luteus syn. nov.

Description of fundatrix and sexual morphs of the Asian apricot aphid, Myzus mumecola (Matsumura, 1917) (Hemiptera: Aphididae).

Fundatrix and sexual morphs of the Asian apricot aphid, Myzus (Myzus) mumecola (Matsumura, 1917) are described from Serbia with original, clarified slide mounts, and illustrations. The morphological identification of sexual morphs was corroborated through COI barcode sequence analysis. The subgeneric position of this species is discussed and the transfer to subgenus Nectarosiphon is proposed.

Bioassay-guided evaluation of antimicrobial properties and profile of bioactive compounds from leaf, peel and mesocarp of four apple cultivars (Malus domestica Borkh.) grown in Serbia: Application of HPTLC-EDA and UHPLC Q-ToF MS techniques.

The aim of this study was to evaluate the antimicrobial properties and profile of bioactive compounds from mesocarp, peel and leaves of four autochthonous apple cultivars against human pathogens, Escherichia coli, Staphylococcus aureus and Bacillus subtilis and the apple pathogen Erwinia amylovora by direct detection on HPTLC plates and subsequent chemometric analysis. UHPLC Q-ToF MS was used for detailed characterization of the bioactive compounds with antimicrobial properties. Leaf extracts showed the highest antibacterial activity against all bacterial strains, followed by peel extracts, while the mesocarp extracts showed only weak and selective inhibition zones for E.

Effects of high-pressure and CaCl pretreatments on the salt taste-enhancing activity of hydrolysate derived from spent hen meat.

Background: High-sodium intake has been proven to bring serious risks to public health. A potential sodium substitute of salt taste-enhancing hydrolysate (STEH) of protein has been focused on recently. The salt taste-enhancing activity (STEA) of STEH still needs to be improved.

The trend in national childhood immunization program coverage throughout Serbian communities in Kosovo and Metohija from 2003 to 2022: pre-COVID-19 period vs. COVID-19 pandemic.

Background: The childhood immunization coverage in Serbian communities in Kosovo after the 1999 armed conflict has not been investigated. The study purpose was to evaluate the trend of immunization coverage with vaccines from the national childhood immunization program in Serbian communities in Kosovo and Metohija from 2003 to 2022.

Methods: Data were retrieved from the annual reports of the Public Health Institute of Kosovska Mitrovica received through notifications from the primary health centers where vaccines are being administered.

Preventing mislabeling of organic white button mushrooms (Agaricus bisporus) combining NMR-based foodomics, statistical, and machine learning approach.

Organic foods are among the most susceptible to fraud and mislabeling since the differentiation between organic and conventionally grown food relies on a paper-trail-based system. This study aimed to develop a differentiation model that combines nuclear magnetic resonance (NMR), statistical approach (principal component analysis - PCA and partial least square discriminant analysis - PLS-DA), and classification artificial neural network (cANN). The model was tested for hydrophilic and lipophilic extracts of Agaricus bisporus.

(R)evolution of Viruses: Introduction to biothermodynamics of viruses.

As of 26 April 2024, the International Committee on Taxonomy of Viruses has registered 14690 virus species. Of these, only several dozen have been chemically and thermodynamically characterized. Every virus species is characterized by a specific empirical formula and thermodynamic properties - enthalpy, entropy and Gibbs energy.

Aortic Valve Replacement vs Clinical Surveillance in Asymptomatic Severe Aortic Stenosis: A Systematic Review and Meta-Analysis.

Background: Current guidelines recommend a strategy of clinical surveillance (CS) for patients with asymptomatic severe aortic stenosis (AS) and a normal left ventricular ejection fraction.

Objectives: The aim of this study was to conduct a study-level meta-analysis of randomized controlled trials (RCTs) evaluating the effect of early aortic valve replacement (AVR) compared with CS in patients with asymptomatic severe AS.

Methods: Studies were quantitatively assessed in a meta-analysis using random-effects modeling.

hand infection following acupuncture: a case report and literature review.

Hand infection caused by atypical mycobacteria is an uncommon condition. We present a case of hand infection caused by in a patient who had undergone acupuncture. The clinical features, treatment, and outcome are described.

Machine learning classification meets migraine: recommendations for study evaluation.

The integration of machine learning (ML) classification techniques into migraine research has offered new insights into the pathophysiology and classification of migraine types and subtypes. However, inconsistencies in study design, lack of methodological transparency, and the absence of external validation limit the impact and reproducibility of such studies. This paper presents a framework of six essential recommendations for evaluating ML-based classification in migraine research: (1) group homogenization by clinical phenotype, attack frequency, comorbidity, therapy, and demographics; (2) defining adequate sample size; (3) quality control of collected and preprocessed data; (4) transparent training, testing, and performance evaluation of ML models, including strategies for data splitting, overfitting control, and feature selection; (5) interpretability of results with clinical relevance; and (6) open data and code sharing to facilitate reproducibility.

Combining treatments for migraine prophylaxis: the state-of-the-art.

Combination treatments for migraine prophylaxis present a promising approach to addressing the diverse and complex mechanisms underlying migraine. This review explores the potential of combining oral conventional prophylactics, onabotulinumtoxin A, monoclonal antibodies (mAbs) targeting the calcitonin gene-related peptide (CGRP) pathway, and small molecule CGRP receptor antagonists (gepants). Among the most promising strategies, dual CGRP inhibition through mAbs and gepants may enhance efficacy by targeting both the CGRP peptide and its receptor, while the combination of onabotulinumtoxin A with CGRP treatments offers synergistic pain relief.

Correction: Density functional theory study of crown ether-magnesium complexes: from a solvated ion to an ion trap.

Correction for 'Density functional theory study of crown ether-magnesium complexes: from a solvated ion to an ion trap' by Katarina Ćeranić , , 2023, , 32656-32665, https://doi.org/10.1039/D3CP03991A.

A pioneering longterm experiment on mesophotic macrofouling communities in the North Atlantic.

The mesophotic zone represents one of our planet's largest and least explored biomes. An increasing number of studies evidence the importance of macrofouling species in marine ecosystems, but information on these communities and the factors influencing their structures at mesophotic depths remain poor. This lack of understanding limits our ability to predict anthropogenic impacts or conduct restoration operations in the mesophotic and the lower boundary of the euphotic zones.

Silica nanoparticles (SiNPs) derived from melon seed husk ameliorate Ni/Al mixture-mediated cognitive impairment in rats.

This study evaluated the protective effects of silica nanoparticles (SiNPs) derived from melon seed husk ash against the neurotoxic effects of common environmental pollutants, aluminum (Al), nickel (Ni), and their combination in Wistar rats. Ninety-one male Sprague Dawley rats (220-250 g; 6-8 weeks old) were divided into 13 experimental groups. Key findings revealed that exposure to nickel, aluminum, or their combination significantly impaired spatial learning and memory, as evidenced by prolonged latency periods in treated rats.

Homophenylalanine-derived benzo[1,4]diazepine-2,5-diones are strong bacterial quorum sensing inhibitors.

Benzo[1,4]diazepines show a large diversity of biological activities and are still commonly used as medications against a broad range of diseases. Within our research in the field of chemo-enzymatic alkaloid synthesis, we developed a synthetic route towards close structural relatives, namely benzo[1,4]diazepine-2,5-diones. Possible antimicrobial activities of these substances are barely known up to date.

Design of an international, phase IV, open-label study of simoctocog alfa in women/girls with hemophilia A undergoing surgery (NuDIMENSION).

Background: Although hemophilia A mainly affects males, carriers (defined as females with hemophilia A, as well as symptomatic or asymptomatic hemophilia A carriers) are at risk of excessive bleeding, particularly during trauma or during surgical procedures. Clinical trials have focused on male patients with severe disease, and data for females are limited. Improved, evidence-based treatment guidelines for management of hemophilia A carriers are required.

Regime switching in coupled nonlinear systems: Sources, prediction, and control-Minireview and perspective on the Focus Issue.

Regime switching, the process where complex systems undergo transitions between qualitatively different dynamical states due to changes in their conditions, is a widespread phenomenon, from climate and ocean circulation, to ecosystems, power grids, and the brain. Capturing the mechanisms that give rise to isolated or sequential switching dynamics, as well as developing generic and robust methods for forecasting, detecting, and controlling them is essential for maintaining optimal performance and preventing dysfunctions or even collapses in complex systems. This Focus Issue provides new insights into regime switching, covering the recent advances in theoretical analysis harnessing the reduction approaches, as well as data-driven detection methods and non-feedback control strategies.