Sequence-Specific Minor Groove Binders in Labeling and Single-Molecule Analysis of DNA.

The ability to address specific sequences within DNA is of tremendous interest in biotechnology and biomedicine. Various technologies have been established over the past few decades, such as nicking enzymes and methyltransferase-directed sequence-specific labeling, transcription activator-like effector nucleases (TALENs), the CRISPR-Cas9 system, and polyamides of heterocycles as sequence-specific DNA minor groove binders. Pyrrole-imidazole polyamides have been reported to recognize predetermined DNA sequences, and some successful attempts have demonstrated their potential in regulating gene expression.

Linguistic validation of the Epworth Sleep Scale among healthy individuals into Moroccan dialect.

Background/aim: We performed a linguistic validation of the original version of the Epworth Sleep Scale (ESS) into the Moroccan dialect according to Guillemin's guidelines.

Materials And Methods: After translating the English version of the ESS according to the standard guidelines, we asked 120 healthy subjects to complete the questionnaire twice with an interval of 10 days. We assess the acceptability and internal reliability by Cronbach's alpha coefficient, while for the test-retest reproducibility we use the interclass correlation coefficient (ICC) (95% confidence interval (CI)) and the kappa coefficient.

Synthesis, Crystal Structures, Genotoxicity, and Antifungal and Antibacterial Studies of Ni(II) and Cd(II) Pyrazole Amide Coordination Complexes.

In this study, we synthesized two coordination complexes based on pyrazole-based ligands, namely 1,5-dimethyl--phenyl-1H-pyrazole-3-carboxamide () and 1,5-dimethyl--propyl-1H-pyrazole-3-carboxamide (), with the aim to investigate bio-inorganic properties. Their crystal structures revealed a mononuclear complex [Ni()](ClO) () and a dinuclear complex [Cd()]Cl (). Very competitive antifungal and anti-Fusarium activities were found compared to the reference standard cycloheximide.

: a rare cause of purulent otorrhoea.

is a Gram-negative, non-fermenting, motile and oxidase-positive bacillus. Its incrimination in human pathology is very rare, although there has been a resurgence in infections in recent years. We report the first case in Morocco of a purulent otorrhoea caused by , resistant to conventional treatment, occurring in a 25-year-old female, afebrile, without deterioration of the general state and possibly acquired during sea bathing.

Synthesis, Spectroscopic Characterization, Antibacterial Activity, and Computational Studies of Novel Pyridazinone Derivatives.

In this work, a novel series of pyridazinone derivatives (-) were synthesized and characterized by NMR (H and C), FT-IR spectroscopies, and ESI-MS methods. All synthesized compounds were screened for their antibacterial activities against (Methicillin-resistant), , , , and . Among the series, compounds and were found to be active against (MRSA), , and with the lowest MIC value range of 3.

Hemorrhagic stroke during the acute phase of myocardial infarction: a rare and difficult situation to manage.

Given the ischemic risk due to the hypercoagulability associated with acute coronary syndromes, the administration of antiplatelet and antithrombotic agents is necessary to prevent intracoronary and postprocedural thrombosis during percutaneous coronary interventions. However, the risk of bleeding, hemorrhagic stroke included, is real, although it has a lower prevalence, and it complicates the management of the coronary event if it happens. We report the case of a 66 years old patient with no prior pathological history who was initially admitted for acute coronary syndromes, complicated by paroxysmal atrial fibrillation that was successfully thrombolysed.

A conceptual model for understanding the zoonotic cutaneous leishmaniasis transmission risk in the Moroccan pre-Saharan area.

Leishmanioses are of public health concern in Morocco, mainly the Zoonotic Cutaneous Leishmaniasis (ZCL) endemic in the Moroccan pre-Saharian area. Transmission of this disease depends on eco-epidemiological and socio-economic conditions. Therefore, a multivariable approach is required to delineate the risk and intensity of transmission.

Acute spontaneous subdural hematoma as an inaugural presentation of systemic lupus erythematosus with acquired factor XIII deficiency: a case report.

Acute spontaneous subdural hematoma is a rare clinical situation. Among its various etiologies, underlying coagulopathy is associated with a considerable risk of mortality. A 43-year-old female patient with no comorbidity and no personal or family history of bleeding disorders, consulted for acute and intense headache.

Pancreatic schwannoma- CT and MRI findings: A rare case report and review of literature.

Introduction: Pancreatic schwannoma (PS) is an extremely rare benign tumor. Here we describe the Computed Tomography (CT) and Magnetic Resonance Imaging (MRI) results of PS in a 59 years old woman, as well as a review of the literature.

Case Presentation: A 59-year-old woman consulted for atypical epigastralgia without fatigue, weight loss or fever.

Guillan barre syndrome in post Covid-19 infection in children.

Introduction: Guillain-Barre syndrome (GBS) is an inflammatory poly-radiculoneuropathy occurring usually two weeks after a viral infection.Covid-19 infection represent with respiratory symptoms, but it might manifests with extra pulmonary symptoms, and especially neurological including Guillain-Barre syndrom.

Case Report: A 3 years old female patient admitted to the intensive care unit for an acute symmetric and progressive ascending quadriparesis two weeks after an upper respiratory infection, the diagnosis of post covid GBS was maintained, and the evolution was favorable after intraveneous immunoglobulins.

ST elevation revealing acute myocarditis with SARS cov 2 infection: Case report.

Introduction: SARS-CoV-2 viral infection can manifested by respiratory symptoms, or other symptoms, such as the cardiovascular manifestations including acute coronary syndrome, pericardial effusion, and heart failure.

Clinical Case: A 51-year-old patient admitted to the emergency room for epigastric pain with no respiratory signs and with an ST-segment elevation inelectrocardiogram that ultimately revealed myocarditis and SARS CoV-2 2 infection.

Conclusion: The clinical manifestations of SARS CoV-2 might be atypical, and the diagnosis might be considered in this pandemic area.

Toxicological Evaluation and Hepatoprotective Efficacy of Rosmarinic Acid-Rich Extract from L.

Exposure to carbon tetrachloride (CCl) induces acute and chronic liver injuries as well as oxidative stress in rats. The present study was designed to evaluate the toxicity of rosmarinic acid-rich extract from (RAE). The acute and subchronic oral toxicity of RAE was evaluated in mice.

Farmer's behaviors toward pesticides use: insight from a field study in Oriental Morocco.

Pesticides are a vital component of modern agriculture. So, these chemical compounds could play a crucial role in maintaining high agricultural productivity. However, high-input intensive agricultural production systems in which more effective and widespread use of pesticides could harm human health and the environment.

Phenolic-Rich Extract from Almond () Hulls Improves Lipid Metabolism in Triton WR-1339 and High-Fat Diet-Induced Hyperlipidemic Mice and Prevents Lipoprotein Oxidation: A Comparison with Fenofibrate and Butylated Hydroxyanisole.

Hyperlipidemia and oxidative stress are risk factors for atherosclerosis. In this study, we investigated the hypolipidemic and anti-lipoprotein oxidation activities of polyphenol-rich extracts from almond hulls using Triton WR-1339 and high-fat diet-induced hyperlipemic mice as experimental models. We demonstrated that the almond hull extract significantly reduced total cholesterol, triglycerides and low-density lipoprotein-related plasma cholesterol (LDL-C) in the two experimental models of hyperlipidemia, but significantly increased high-density lipoprotein-related plasma cholesterol (HDL-C).

The cosmic ray shadow of the Moon observed with the ANTARES neutrino telescope.

One of the main objectives of the ANTARES telescope is the search for point-like neutrino sources. Both the pointing accuracy and the angular resolution of the detector are important in this context and a reliable way to evaluate this performance is needed. In order to measure the pointing accuracy of the detector, one possibility is to study the shadow of the Moon, i.

Screening of the unrecognised bipolar disorders among outpatients with recurrent depressive disorder: a cross-sectional study in psychiatric hospital in Morocco.

The bipolar disorder is often misdiagnosed in particular among outpatients with recurrent depression. Indeed, this work confirmed that the unrecognised bipolar disorder is common among depressed outpatients, which were younger, unemployed, single or divorced with a low socio-economic level. These socio-demographics data gives us an idea about the disability experienced by the unknown bipolar patients.

An algorithm for the reconstruction of high-energy neutrino-induced particle showers and its application to the ANTARES neutrino telescope.

A novel algorithm to reconstruct neutrino-induced particle showers within the ANTARES neutrino telescope is presented. The method achieves a median angular resolution of [Formula: see text] for shower energies below 100 TeV. Applying this algorithm to 6 years of data taken with the ANTARES detector, 8 events with reconstructed shower energies above 10 TeV are observed.

Sperm whale long-range echolocation sounds revealed by ANTARES, a deep-sea neutrino telescope.

Despite dedicated research has been carried out to adequately map the distribution of the sperm whale in the Mediterranean Sea, unlike other regions of the world, the species population status is still presently uncertain. The analysis of two years of continuous acoustic data provided by the ANTARES neutrino telescope revealed the year-round presence of sperm whales in the Ligurian Sea, probably associated with the availability of cephalopods in the region. The presence of the Ligurian Sea sperm whales was demonstrated through the real-time analysis of audio data streamed from a cabled-to-shore deep-sea observatory that allowed the hourly tracking of their long-range echolocation behaviour on the Internet.

Vasorelaxant and anti-platelet aggregation effects of aqueous Ocimum basilicum extract.

Aim Of The Study: In this work the endothelium-dependant vasorelaxant and anti-platelet aggregation activities of an aqueous extract from Ocimum basilicum were studied.

Materials And Methods: The vasorelaxant effect was undertaken in thoracic aorta from three experimental groups of rats: one of them (NCG) fed with standard diet, the second (HCG) with hypercholesterolemic diet (HCD) and the third (BTG) with hypercholesterolemic diet together with an intragastric administration of Ocimum basilicum extract at a dose of 0.5 g/kg body weight for a period of 10 weeks.