A novel human cytochrome P450 gene (P450IIB): chromosomal localization and evidence for alternative splicing.

We have isolated from a single human liver cDNA library two clones which are highly homologous (78% over the coding region) to the major phenobarbital-inducible P450 from rat (P450IIB1). This is the first direct demonstration of the presence of the P450IIB gene subfamily in humans. This subfamily is much less extensive than the rodent homologues, but does appear to contain at least two genes.

The effect of troponin C removal on the Ca2+-sensitive binding of Mg2+ AMPPNP to myofibrils.

It was previously shown that when rabbit skeletal myofibrils are titrated with Mg2+ AMPPNP under conditions that result in the dissociation of cross-bridges from the thin filaments (i.e. 50% ethylene glycol, 0 degrees C), Ca2+-sensitive, biphasic binding is observed.

Containment system for the preparation of vitrified-hydrated virus specimens.

A guillotine-type quick freezing device and a bio-hazard containment box have been designed, constructed, and used to prepare vitrified-hydrated specimens of viruses in their native environment. Special design considerations include the preservation of the specimen in its natural state in vitrified ice, prevention of virus aerosols escape, and control of the potentially explosive air-coolant vapor mixtures.

Evidence that the catalytic differences of two structurally homologous forms of cytochrome P-450 relate to their heme environment.

Cytochromes P-450 PB3a and PB3b, which appear to be equivalent to forms b and e described by Ryan et al. [Ryan, D.E.

Influence of etofibrate on low density lipoprotein metabolism.

This study examined the effect of single dose etofibrate (1.0 g/day) on plasma lipids and lipoproteins in a group of eleven hypercholesterolemic individuals. The drug lowered plasma triglyceride and cholesterol by 32% and 14%, respectively (P less than 0.

High resolution cryo system designed for JEM 100CX electron microscope.

A high resolution (3.5 A) cold stage operated at 123 K and an improved anti-contamination device have been constructed and operated in a top-entry JEM 100CX electron microscope. High resolution electron diffraction patterns and images of hydrated tobacco mosaic virus particles in vitrified ice have been recorded with the use of this cryo system.

Lactate dehydrogenase isoenzymes of human semen.

1. A lactate dehydrogenase isoenzyme present in human spermatozoa and semen was isolated and characterized biochemically in term of its pH for optimum activity and by means of K(m) values for lactate, NAD(+) and NAD analogues. The results were compared with those obtained with the human heart-type and the liver-type lactate dehydrogenase isoenzymes.