Identification, structure, and agonist design of an androgen membrane receptor.

Androgens, such as 5α-dihydrotestosterone (5α-DHT), regulate numerous functions by binding to nuclear androgen receptors (ARs) and potential unknown membrane receptors. Here, we report that the androgen 5α-DHT activates membrane receptor GPR133 in muscle cells, thereby increasing intracellular cyclic AMP (cAMP) levels and enhancing muscle strength. Further cryoelectron microscopy (cryo-EM) structural analysis of GPR133-Gs in complex with 5α-DHT or its derivative methenolone (MET) reveals the structural basis for androgen recognition.

Effects of Fisetin and Nicorandil on adjuvant-induced rheumatoid arthritis in rats: Emerging role of TLR4/NF-κB-induced Pyroptosis, Nrf-2/HO-1, and OPG/RANKL pathways.

Aim And Background: Our study explored the novel mechanisms implicated in the anti-rheumatic potential of fisetin and/or nicorandil (NIC) intervention.

Methods And Materials: Fifty male rats were categorized into; control, rheumatoid arthritis (RA), fisetin-treated RA, NIC-treated RA, and co-treated RA groups. We assessed paw thickness, arthritis indices, serum CRP, RF, OPG, RANKL, and gene expressions of synovial TLR4, NLRP3, caspase-1, GSDMD, Nrf-2, and HO, along with synovial histopathology and NF-κB immunoreactivity.

Recurrent ulcerative tubular apocrine adenoma of the eyelid mimicking eyelid malignancy: A case report and literature review.

Introduction: The eyelid is a common site for cutaneous tumors; however, tubular apocrine adenoma is a rare presentation. The clinical presentation is variable and surgical excision is effective with low risk for recurrence.

Presentation Of Case: We report a 40-year-old female with recurrent tubular apocrine adenoma of eyelid mimicking eyelid malignancy.

Current trends and future potential in the detection of avian coronaviruses: An emphasis on sensors-based technologies.

Infectious bronchitis virus (IBV), an avian coronavirus, member of the genus Gammacoronavirus, poses significant threats to poultry health, causing severe respiratory, reproductive, and renal infections. The genetic diversity of IBV, driven by mutations, recombination and deletions, has led to the emergence of numerous serotypes and genotypes, complicating both diagnosis and control measures. Rapid and accurate diagnostic tools are essential for effective disease management and minimizing economic losses.

Modulation of plant carbon and nitrogen metabolism by novel actinobacteria Rhodospirillum sp. to combat galaxolide toxicity in barley and maize plants.

The phytotoxic effect of cosmetics such as galaxolide (HHCB) has been investigated, however, their metabolic basis of this impact is still obscure. Thus, we investigated the effect of HHCB on the biomass accumulation, photosynthesis, primary and secondary metabolites in two species from different functional groups i.e.

Modelling assisted phytoextraction of heavy metals from tannery origin leachate.

The toxic tannery solid waste leachate (TSWL) containing heavy metals is generated after the percolation of rainwater in openly dumped tannery solid waste (TSW) which poses a serious threat to the surroundings by leaching down and bioaccumulation. For its management, the phytoextraction potential of Pistia stratiotes L. and Spirodela polyrhiza L.

Photochemical bomb: Precision nuclear targeting to activate cGAS-STING pathway for enhanced bladder cancer immunotherapy.

Activating the cGAS-STING pathway presents a promising strategy to enhance the innate immunity and combat the immunosuppressive tumor microenvironment. One key mechanism for triggering this pathway involves the release of damaged DNA fragments caused by nuclear DNA damage. However, conventional cGAS-STING agonists often suffer from limited nucleus-targeting efficiency and potential biotoxicity.

McDonald criteria application by German neurologists suggests a need for further training.

Background: McDonald criteria (MC) are a globally accepted standard for the diagnosis of multiple sclerosis (MS). Misdiagnosis of MS is a common problem that has significant clinical consequences for patients. Misapplication of MC is a potential source of MS misdiagnosis.

Exploring acemannan-loaded nanogel formulation for the treatment of IMQ-induced psoriasis-like inflammation: In vitro characterization and in vivo efficacy assessment.

This study aimed to explore a nanogel formulation containing acemannan as a carrier for the treatment of psoriasis-like skin inflammation. Several acemannan concentrations, such as F1 (2.5 %) and F2 (5 %), were used to prepare the nanogel formulation by homogenization.

Phenylboronic acid functionalized magnetic ferroferric oxide nanoparticles and capillary electrophoresis for efficient isolation of flavonoid compounds from leaves of Lonicera japonica Thunb.

Flavonoids are bioactive components in natural products, which possess anti-inflammatory, antibacterial, antioxidant, and cardiovascular protective properties. However, due to the complexity and low content of the components in these samples, developing rapid and sensitive methods for the isolation and extraction of flavonoids still remains a challenge in medical and food science. Herein, a 4-formylphenylboronic acid functionalized magnetic FeO nanomaterial (FeO@FPBA) was synthesized and applied as a sorbent of magnetic solid-phase extraction (MSPE) to covalently extract flavonoids from leaves of Lonicera japonica Thunb.

Multi-signal fluorescent probe for simultaneous differentiation and imaging of Hg, Cys, Hcy, and GSH in living cells and zebrafish.

The Mercury (II) ion (Hg²⁺) is a toxic heavy metal that threatens biological systems by inducing oxidative stress and disrupting the redox balance. Biothiols such as cysteine (Cys), homocysteine (Hcy), and glutathione (GSH) are critical in maintaining redox homeostasis and are implicated in numerous physiological and pathological processes. Understanding the complex interactions between Hg²⁺ and biothiols requires molecular tools capable of simultaneous detection.

Joint exposure to multiple air pollutants and residual cardiovascular risk in hypertension.

Background: Despite the widespread availability of antihypertensive medications, residual cardiovascular risk of hypertension remained high. Limited studies have investigated the link between air pollution, particularly joint exposure to multiple air pollutants, with residual cardiovascular risk of hypertension.

Methods: 1981 hypertensive patients (≥ 18 years) from an ongoing longitudinal cohort in China, were enrolled between 2013 and 2019.

Adverse Childhood Experiences and Accelerated Epigenetic Aging in the Hispanic Community Health Study/Study of Latinos: Nativity as an Effect Modifier.

Background: Whether adverse childhood experiences (ACEs) are associated with accelerated epigenetic aging over time among the Hispanic/Latino population remains unknown. This study examined the longitudinal association between ACEs and epigenetic age acceleration (EAA), as well as potential effect modifiers, among a sample of Hispanic/Latino adults.

Methods: We analyzed 960 Hispanic/Latino adults with DNA methylation (DNAm) profile data from two visits (approximately six years apart) sampled from the Hispanic Community Health Study/Study of Latinos (HCHS/SOL).

A DFT study of pure and Si-decorated boron nitride allotrope Irida monolayer as an effective sensor for hydroxyurea drug.

Investigating effective nanomaterials for the detection of hydroxyurea anticancer drugs is essential for promoting human health and safeguarding environmental integrity. This research utilized first-principles estimations for examining the adhesion and electronic characteristics of hydroxyurea (HU) on both pristine and Si-decorated innovative two-dimensional boron nitride allotrope, known as Irida analogous (Ir-BNNS). Analyzing the adsorption energy revealed that the HU molecule has a significant interaction (E = -1.

Profiling microRNA expression differentiates monozygotic twins in peripherical blood by droplet digital PCR.

It is challenging to distinguish monozygotic (MZ) twins using traditional autosomal STR genotyping due to their nearly identical genomes. As an important kind of small non-coding RNAs, microRNAs (miRNAs) are essential regulators of gene expression and considered as excellent biomarkers due to their resistance to degradation. Moreover, droplet digital PCR (ddPCR) has emerged as a powerful technique for detecting gene mutations and pathogenic microorganisms, owing to its sensitivity and reliability.

Comparative evaluation of left atrial size in healthy cats measured by two-dimensional echocardiography and cardiovascular MRI.

Objectives: This study aimed to assess left atrial (LA) size in healthy cats using cardiovascular MRI (cMRI) and to compare this with LA size assessed by two-dimensional echocardiography. The hypothesis was that cMRI would accurately determine LA size in domestic cats.

Methods: A prospective comparative study was performed.

Body mass index changes and predictors among adults living with HIV/AIDS who are on anti-retroviral therapy at Chiro General Hospital, Eastern Ethiopia: a facility-based retrospective cohort study.

Background: Human immunodeficiency virus continues to be a major global public health issue. Body mass index is a general indicator of nutritional status and has emerged as a powerful predictor of morbidity and mortality among adult PLHIV initiating antiretroviral therapy in resource-limited settings. However, there is a dearth of information regarding longitudinal changes in body mass index and its predictors among adult PLHIV in Ethiopia, particularly in the study area.

Biofilm characterisation of Mycoplasma bovis co-cultured with Trueperella pyogenes.

Mycoplasma pneumonia, caused by Mycoplasma bovis (Mycoplasmopsis bovis; M. bovis), is linked with severe inflammatory reactions in the lungs and can be challenging to treat with antibiotics. Biofilms play a significant role in bacterial persistence and contribute to the development of chronic lesions.

Participation of COX2/mPGES1/PGE2 in mouse and human endometrial stromal decidualization.

Background: Prostaglandin E2 (PGE2) is vital for embryo implantation and decidualization. Whether COX2/mPGES1/PGE2 pathway is essential for mouse and human decidualization remains unclear.

Results: This study showed that mPGES1 was highly expressed in the mouse uterus's subluminal stromal cells at the implantation site.

Development and validation of the Women Autonomy Scale for measuring psychosocial freedom from conventional gender roles.

Background: The concepts of masculinity and femininity have historically shaped gender roles, leading to inequality and gender-based discrimination. Women's autonomy, defined as the ability to make independent choices across various life domains, remains inadequately measured by existing scales. This study addresses this gap by developing and validating the Women Autonomy Scale (WAS).

Intention to use eLearning-based continuing professional development and its predictors among healthcare professionals in Amhara region referral hospitals, Ethiopia, 2023: using modified UTAUT-2 model.

Background: Continuing Professional Development (CPD) is provided through in-service programs organized based on competency development and lifelong learning for healthcare professionals to stay fit with the required knowledge and skills. However, healthcare professionals' financial constraints and tight schedules sending them away from the workplace for CPD training is a challenge. eLearning is becoming the best solution to overcome those barriers and create accessible, efficient, flexible, and convenient professional development.

Gene expression of psychiatric disorder-related kinesin superfamily proteins (Kifs) is potentiated in alternatively activated primary cultured microglia.

Objective: Reactivity of microglia, the resident cells of the brain, underlies innate immune mechanisms (e.g., injury repair), and disruption of microglial reactivity has been shown to facilitate psychiatric disorder dysfunctions.

The attitudes of physicians toward nurse prescribing rights: a cross-sectional study.

Background: Nursing prescribing rights have been proposed in many countries, with physicians' attitudes playing an important role. This study aimed to investigate the attitudes of physicians toward nurse prescribing rights.

Methods: A cross-sectional study of 112 Chinese physicians was conducted from January to March 2024.

Graft-repositioning technique using infusion and small bubbles during Descemet's membrane endothelial keratoplasty.

Background: Descemet's membrane endothelial keratoplasty (DMEK) is a highly effective procedure for corneal endothelial dysfunction; however, once a DMEK graft is deployed, repositioning can be challenging. Therefore, this study aimed to evaluate the efficacy of a technique that utilizes infusion and small air bubbles to reposition a misaligned deployed graft.

Methods: This retrospective interventional case series enrolled patients who underwent DMEK between January 2022 and July 2023, including cases where the DMEK graft was attached and unfolded in off-center positions".