Transcriptomic effects of Tet-on and mifepristone-inducible systems in mouse liver.

Control of transgene expression from long-term expression vectors can be achieved with inducible and regulated promoters. The two most commonly used inducible systems employ doxycycline or mifepristone as the drug activating a silent trans-activator, which is expressed from a constitutive promoter. We evaluated the alterations provoked by constitutive expression in the liver of rtTA2(S)-M2 (rtTA2; second-generation reverse tetracycline-controlled trans-activator) and GLp65, which are the trans-activators of the doxycyline- and mifepristone-inducible systems, respectively.

Effects of protein vs. carbohydrate-rich diets on fuel utilisation in obese women during weight loss.

Background: Energy restriction is a common therapeutic approach for weight reduction, but the most effective macronutrient distribution of the hypoenergetic diet as well as the role of the metabolic processes involved require further investigations.

Objective: To study the effect of a high protein (HP) hypoenergetic diet compared with a high carbohydrate (HC) hypoenergetic diet on fuel utilisation changes during the weight loss process in obese women.

Design: Eleven obese (BMI>30) women were randomly assigned to a 10w dietary intervention study comparing HP (30% protein) or HC (55% carbohydrate) energy restricted diets providing 30% energy fat content.

A comparison of Portuguese and American patients with hematological malignancies: a cross-cultural survey of health-related quality of life.

This study investigates health-related quality of life (QoL) differences between 98 Portuguese and 109 US American outpatients with hematological malignancies. These two national groups of patients were characterized in terms of patients' QoL, and socio-demographic and clinical variables. Differences were found for several socio-demographic variables (race, marital and job status, urban residence, diagnosis, age, education, and household size).