Spinal muscular atrophy in Holstein-Friesian calves.

The clinical and neuropathological findings of spinal muscular atrophy (SMA) in Holstein-Friesian calves are described in four females and one male from a dairy farm composed of 150 cows and 2 breeding bulls. Locomotion difficulties started at the age of 15 days, and progressed to paraparesis and tetraparesis in 2 weeks. Signs consistent with denervation were revealed with electromyography.

Prevalence of Cryptosporidium infections in pigs in Aragón (northeastern Spain).

Faecal samples from 620 pigs randomly selected from 27 farms throughout Aragón were examined to determine the prevalence of Cryptosporidium infections. Detection of oocysts was performed using the ethyl-acetate stool concentration method and the modified Ziehl-Neelsen technique. Cryptosporidium parvum oocysts were identified in 136 (21.

Comparison of an acid-fast stain and a monoclonal antibody-based immunofluorescence reagent for the detection of Cryptosporidium oocysts in faecal specimens from cattle and pigs.

A commercially available direct immunofluorescence (IF) assay with monoclonal antibodies (Monofluo Kit Cryptosporidium, Diagnostics Pasteur, France) was compared with the modified Ziehl-Neelsen (MZN) acid-fast technique for the detection of Cryptosporidium oocysts in faecal samples from cattle and pigs. Stool specimens individually collected from 108 bovines and 90 pigs were examined in a blind test. The results of the two procedures corresponded (both positive or negative) in 102 (94.

Prevalence of Cryptosporidium and Giardia infections in cattle in Aragón (northeastern Spain).

Faecal samples from 554 bovines randomly selected at 30 farms in Aragón were examined to investigate the prevalence of Cryptosporidium and Giardia infections. C. parvum oocysts were identified by using the Ziehl-Neelsen modified technique in 109 (19.

Comparison of oocyst shedding and the serum immune response to Cryptosporidium parvum in cattle and pigs.

A comparison was made between oocyst shedding and the presence of specific serum IgG antibodies to Cryptosporidium parvum in 108 bovines and 90 pigs. Oocysts were detected by a commercial immunofluorescence assay in feces from 26.8% of bovines and 34.

The role of radiation therapy in vertebral hemangiomas without neurological signs.

We describe 5 patients with vertebral haemangiomas treated by radiotherapy of 30-40 Gy, 2-3 Gy/day. The management of vertebral haemangiomas is discussed.

Alizarin-9-imine as indicator in acetonitrile and in pyridine media.

The behaviour of alizarin-9-imine as an indicator in acetonitrile and pyridine media has been studied. The protonation constant in acetonitrile, K(I) = a(Hi) + a(H(+)) . a(I) = 10(-3.

9-Imino derivatives of dihydroxyanthraquinones as analytical reagents.

The synthesis, characteristics, properties and reactions with metallic ions of the reagents isticin-9-imine, alizarin-9-imine and 3-sulphoalizarin-9-imine have been studied.