TClC effectively suppresses the growth and metastasis of NSCLC via polypharmacology.

Despite significant advances in targeted therapies and immunotherapies, non-small cell lung cancer (NSCLC) continues to present a global health challenge, with a modest five-year survival rate of 28 %, largely due to the emergence of treatment-resistant and metastatic tumors. In response, we synthesized a novel bioactive compound, ethyl 6-chlorocoumarin-3-carboxylyl L-theanine (TClC), which significantly inhibited NSCLC growth, epithelial mesenchymal transition (EMT), migration, and invasion and tumor growth and metastasis without inducing toxicity. TClC disrupts autocrine loops that promote tumor progression, particularly in stem-like CD133-positive NSCLC (CD133+ LC) cells, which are pivotal in tumor metastasis.

Flower-like tailored carbon nitride oligomer as an excellent aggregation-induced electrochemiluminescence emitter for sensitive immunoassay of neuron-specific enolase via dual quenching by bimetallic phenolic networks.

The adjustment of the electrochemiluminescence (ECL) of polymeric carbon nitride (CN) is essential for its application in sensitive immunoassays. However, such modification through aggregation-induced emission (AIE) has not yet been reported. Herein, aggregation-induced ECL in CN oligomer (CNO) was induced through the introduction of a rotatable imine moiety, with the resulting material exhibiting excellent performance in the targeted immunodetection of neuron-specific enolase.

Spatiotemporal dynamics of early oogenesis in pigs.

Background: In humans and other mammals, the process of oogenesis initiates asynchronously in specific ovarian regions, leading to the localization of dormant and growing follicles in the cortex and medulla, respectively; however, the current understanding of this process remains insufficient.

Results: Here, we integrate single-cell RNA sequencing (scRNA-seq) and spatial transcriptomics (ST) to comprehend spatial-temporal gene expression profiles and explore the spatial organization of ovarian microenvironments during early oogenesis in pigs. Projection of the germ cell clusters at different stages of oogenesis into the spatial atlas unveils a "cortical to medullary (C-M)" distribution of germ cells in the developing porcine ovaries.

Gestational diabetes mellitus causes genome hyper-methylation of oocyte via increased EZH2.

Gestational diabetes mellitus (GDM), a common pregnancy disease, has long-term negative effects on offspring health. Epigenetic changes may have important contributions to that, but the underlying mechanisms are not well understood. Here, we report the influence of GDM on DNA methylation of offspring (GDF1) oocytes and the possible mechanisms.

Elucidating Manganese Single-Atom Doping: Strategies for Fluorescence Enhancement in Water-Soluble Red-Emitting Carbon Dots and Applications for FL/MR Dual Mode Imaging.

The absence of the enhancement of fluorescence in carbon dots (CDs) through doping with transition metal atoms (TMAs) hinders the advancement of multi-modal bio-imaging CDs with high photoluminescence quantum yield (PLQY). Herein, Mn-atomically-doped R-CDs (R-Mn-CDs) with a high PLQY of 41.3% in water is presented, enabling efficient in vivo dual-mode fluorescence/magnetic resonance (MR) imaging.

Intense and vibrant color construction and functionalization for protein macromolecule/polyamide two-component fabric by sustainable microbial nano prodigiosins based on adjustable pigment allocation.

Silk/polyamide fabric inherits the advantages of natural and synthetic fibers, making them remarkable in textile and garment field. However, the use of synthetic chemicals for color construction and functionalization of silk/polyamide fabrics is problematic because of their non-renewable resources and harmful effects on the environment. Furthermore, achieving even color construction of silk and polyamide fibers in one bath is challenging due to their significant differences in chemical structure and surface properties.

The CYLD-PARP1 feedback loop regulates DNA damage repair and chemosensitivity in breast cancer cells.

Poly(ADP-ribose) polymerase 1 (PARP1) plays a crucial role in DNA repair and genomic stability maintenance. However, the regulatory mechanisms governing PARP1 activity, particularly through deubiquitination, remain poorly elucidated. Using a deubiquitinase (DUB) library binding screen, we identified cylindromatosis (CYLD) as a bona fide DUB for PARP1 in breast cancer cells.

Electron Transport Chains Promote Selective Photocatalytic Conversion of CO to Methanol.

The photocatalytic conversion of carbon dioxide (CO) into "liquid sunshine" methanol (CHOH) using semiconductor catalysts has garnered significant attention. Increasing the number of effective electrons and regulating reaction pathways is the key to improving the activity and selectivity of CHOH. Due to the electron transport properties of semiconductor heterojunctions and reduced graphene oxide (rGO), a CoS/CoS-rGO nanocomposite was constructed and applied to the photocatalytic reduction of CO to CHOH.

Focused ion beam (FIB) prepared microtextured microneedle array capable of delivering drugs through punctured skin.

Microtextured microneedles are tiny needle-like structures with micron-scale microtextures, and the drugs stored in the microtextures can be released after entering the skin to achieve the effect of precise drug delivery. In this study, the skin substitution model of Ogden's hyperelastic model and the microneedle array and microtexture models with different geometrical parameters were selected to simulate and analyse the flow of the microtexture microneedle arrays penetrating the skin by the finite-element method, and the length of the microneedles was determined to be 200 μm, the width 160 μm, and the value of the gaps was determined to be 420 μm. A four-pronged cone was chosen as the shape of microneedles, and a rectangle was chosen as the shape of the drug-carrying microneedle.

Ratiometric sensor based on Ag-mediated luminescence of Tb-DNA complexes for visual detection of 4-aminophenol.

Development of accurate, convenient and portable methods for monitoring 4-aminophenol (4-AP) is extremely important because of its strong toxicity. Here, a ratiometric fluorescence sensor based on Ag-enhanced luminescence of Tb-DNA complexes has been presented for the detection of 4-AP. The luminescence of Tb-DNA complexes is enhanced about 30 times by Ag, which can trigger energy transfer from DNA to Tb more efficiently.

Concurrent Severe Fever with Thrombocytopenia Syndrome Virus Outbreaks on Multiple Fox Farms, China, 2023.

The role of farmed animals in the viral spillover from wild animals to humans is of growing importance. Between July and September of 2023 infectious disease outbreaks were reported on six Arctic fox () farms in Shandong and Liaoning provinces, China, which lasted for 2-3 months and resulted in tens to hundreds of fatalities per farm. Severe Fever with Thrombocytopenia Syndrome Virus (SFTSV) was identified in tissue/organ and swab samples from all the 13 foxes collected from these farms.

Effective multicolor visual biosensor for ochratoxin A detection enabled by DNAzyme catalysis and gold nanorod etching.

A novel detection technique is introduced that offers sensitive and reliable ochratoxin A (OTA) detection. The method leverages the etching of gold nanorods (AuNRs) stabilized by hexadecyl trimethyl ammonium bromide (CTAB) using the oxidized form of 3,3',5,5'-tetramethyl benzidine sulfate (TMB), creating a susceptible multicolor visual detection system for OTA. The visual detection is enabled by Mg-assisted DNAzyme catalysis combined with the catalytic hairpin assembly (CHA) signal amplification strategy.

Lower toxicity of HFPO-DA compared to its predecessor PFOA to the earthworm Eisenia fetida: Evidence from oxidative stress and transcriptomic analysis.

Hexafluoropropylene oxide dimer acid (HFPO-DA), an emerging perfluoroalkyl substance (PFAS) that is replacing traditional PFASs, has a wide range of industrial applications and has been detected globally in the environment. However, it remains unclear whether HFPO-DA, is genuinely less toxic than perfluorooctanoic acid (PFOA) in terms of soil environmental hazards. Therefore, this study aimed to compare differences in toxicity between PFOA and its substitute, HFPO-DA, in a common species of earthworm, Eisenia fetida.

Controllable assembly of three-dimensional SERS substrate for highly sensitive detection of thiram residues in vegetables.

In this work, a series of three-dimensional (3D) SERS substrate were successfully fabricated by assembling silver nanoparticles (AgNPs) onto a porous gelatin sponge (GS) for highly sensitive thiram residues detection in vegetables. These 3D micro-nanostructures could induce the sufficient surface plasmon resonance (SPR) effect of noble metals on their surface and achieve high enrichment of pollutant molecules. As crystal violet (CV) was used as a probe molecule, the lowest CV solution could be detected at 10 M, and the enhancement factor (EF) was calculated to be 9.

Evaluations of the Perturbation Resistance of the Deep-Learning-Based Ligand Conformation Optimization Algorithm.

In recent years, the deep learning (DL) technique has rapidly developed and shown great success in scoring the protein-ligand binding affinities. The protein-ligand conformation optimization based on DL-derived scoring functions holds broad application prospects, for instance, drug design and enzyme engineering. In this study, we evaluated the robustness of a DL-based ligand conformation optimization protocol (DeepRMSD+Vina) for optimizing structures with input perturbations by examining the predicted ligand binding poses and scoring.

Comparison of the combined toxicity of PFOA and emerging alternatives: A comprehensive evaluation of oxidative damage, apoptosis and immunotoxicity in embryonic and adult zebrafish.

Perfluorohexanoic acid (PFHxA) and perfluorobutanoic acid (PFBA) are widely used substitutes to perfluorooctanoic acid (PFOA). Whether these substitutes are less toxic than PFOA remains unclear owing to differences in the experimental methods, test organisms, and other experimental conditions in previous studies. The present study selected 0.

Inhibitory Effect of PRMT5/MTA Inhibitor on MTAP-Deficient Glioma May Be Influenced by Surrounding Normal Cells.

Background: Methylthioadenosine phosphorylase (MTAP) and protein arginine methyltransferase 5 (PRMT5) are considered to be a synthetic lethal pair of targets, due to the fact that deletion of MTAP leads to massive production of methylthioadenosine (MTA) decreasing the activity of PRMT5. In vitro and in vivo experiments have demonstrated that MRTX1719, a small molecule that selectively binds PRMT5/MTA complex, significantly inhibits the proliferation of MTAP-deficient tumors and has a weak toxic effect on normal cells. However, it has been reported that MTAP-deleted tumors did not significantly accumulate MTA in vivo due to metabolism of MTA by MTAP-expressing stroma, which might lead to a diminished anti-cancer effect of MRTX1719.

Cu-MOF-based targeted nanomedicine utilizing biorthogonally catalyzed chemotherapy and chemodynamic therapy with spatiotemporal orchestration to treat hepatocellular carcinoma.

A nanomedicine was developed using a Cu-based metal-organic framework (MOF) to co-deliver Cu(I) and prodrugs for targeted biorthogonally catalyzed chemotherapy and chemodynamic therapy of hepatocellular carcinoma.

Saffron extract alleviates D-gal-induced late-onset hypogonadism by activating the PI3K-Akt-Nrf2 signaling pathway.

Ethnopharmacological Relevance: Late-onset hypogonadism (LOH) is a common age-related condition in men, characterized by a decline in serum testosterone and a range of associated signs and symptoms, and is classified as impotence in traditional medicine. Saffron is the dried stigma of Crocus sativus L., which is used in traditional medicine as an aphrodisiac.

Copper-based fluorescent nanozyme used to construct a ratiometric sensor for visual detection of thiophanate methyl.

Although nanozyme has shown great potential in designing fluorescent assays for pesticide residue, most of them are based on single emission, thus affecting the detection accuracy. Herein, a copper-based fluorescent nanozyme (Cu-BH) synthesized with dual-ligand, integrating fluorescence and oxidase-mimic into one spherical nanomaterial, was used firstly to establish a ratiometric approach for visual detection of thiophanate methyl (TM). Cu-BH possesses excellent oxidase-like activities, triggering the oxidation of colorless o-phenylenediamine (OPD) into yellow luminescent products (oxOPD, λem = 564 nm).

Aggregation-Induced Emission Carbon Dot-Based Multicolor Circularly Polarized Afterglow with a High Luminescence Dissymmetry Factor.

Carbon dots (CDs) with circularly polarized afterglow (CPA) materials have drawn increasing attention as cutting-edge research in the field of chiral luminescence owing to their promising applications in various fields. However, due to the weak optical activity of chiral CDs and the limited afterglow color of phosphorescent CDs, it is still a formidable challenge to construct multicolor CD-based CPA materials with a high luminescence dissymmetry factor (). Herein, positively charged aggregation-induced emission (AIE) CDs were prepared using dithiosalicylic acid and ionic liquid as precursors.

Targeted thermosensitive liposomes loaded with gold nanoparticles and temozolomide hexadecanoate for the synergistic photothermal-chemotherapy treatment of glioblastoma.

Glioblastoma (GBM) is a highly aggressive malignant brain tumour which presents a significant challenge due to the limited effectiveness of current surgical and chemotherapeutic approaches. In this study, we have developed TMZ16e and gold nanoparticles coencapsulated thermosensitive liposomes modified with anti-EphA3 (anti-EphA3-TMZ16e-GNPs-TSL) delivered via the intranasal route to achieve photothermal chemotherapy (PCT) for improving the therapeutic effects of GBM. The prepared anti-EphA3-TMZ16e-GNPs-TSL were spherical with a particle size of 173.

Ti-co-Ce oxide decorated chitosan fiber oxidase mimic identified for portable monitoring of S, CrO and Fe assisted with a smartphone.

Environmental safety and protection is one of the most concerned topics nowadays. To conveniently monitor toxic S/CrO and to regulate bioactive Fe, Ti-co-Ce oxide decorated chitosan fiber (Ti-co-Ce ox@CC) was developed using microwave-assisted hydrothermal method. The integration of chitosan fiber and nano Ti-co-Ce oxide endowed Ti-co-Ce ox@CC with superior oxidase-like activity and improved water-dispersibility.

Methane production related to microbiota in dairy cattle feces.

Methane (CH) emission from livestock feces, led by ruminants, shows a profound impact on global warming. Despite this, we have almost no information on the syntrophy of the intact microbiome metabolisms, from carbohydrates to the one-carbon units, covering multiple stages of ruminant development. In this study, syntrophic effects of polysaccharide degradation and acetate-producing bacteria, and methanogenic archaea were revealed through metagenome-assembled genomes from water saturated dairy cattle feces.