Temperature-based investigation of rhamnolipids congeners production by the non-pathogenic Burkholderia thailandensis E264 using LC-QToF-MS metabolomics.

Introduction: Burkholderia thailandensis E264 is a non-pathogenic soil bacterium that produces rhamnolipids (RLs), which are utilised in various fields. Although studies have illustrated changes in RLs congeners in response to environmental factors, studies on the influence of temperature on the RLs congeners produced by B. thailandensis E264 are scarce.

Thermo-Responsive Polymer-siRNA Conjugates Enabling Artificial Control of Gene Silencing around Body Temperature.

Purpose: Controlling small interfering RNA (siRNA) activity by external stimuli is useful to exert a selective therapeutic effect at the target site. This study aims to develop a technology to control siRNA activity in a thermo-responsive manner, which can be utilized even at temperatures close to body temperature.

Methods: siRNA was conjugated with a thermo-responsive copolymer that was synthesized by copolymerization of N-isopropylacrylamide (NIPAAm) and hydrophilic N,N-dimethylacrylamide (DMAA) to permit thermally controlled interaction between siRNA and an intracellular gene silencing-related protein by utilizing the coil-to-globule phase transition of the copolymer.

Development and evaluation of ciprofloxacin-bacterial cellulose composites produced through in situ incorporation method.

This paper describes the interaction and properties of bacterial cellulose (BC)-ciprofloxacin composites synthesized by in situ incorporation method. Ciprofloxacin's susceptibility to BC's producer, , was first tested to determine its inhibitory activity against the bacteria. In situ incorporation method was performed by introducing 0.