Development, Optimization, and Validation of a Quantitative PCR Assay for Detection in Tick, Wildlife, and Human Samples.

Tick-borne pathogens are growing in importance for human and veterinary research worldwide. We developed, optimized, and validated a reliable quantitative PCR (qPCR; real-time PCR) assay to assess Borrelia burgdorferi infection by targeting two B. burgdorferi genes, and .

X*Y females exhibit steeper reproductive senescence in the African pygmy mouse.

A wave of studies has recently emphasized the influence of sex chromosomes on both lifespan and actuarial senescence patterns across vertebrates and invertebrates. Basically, the heterogametic sex (XY males in XX/XY systems or ZW females in ZW/ZZ systems) typically displays a lower lifespan and a steeper rate of actuarial senescence than the homogametic sex. However, whether these effects extend to the senescence patterns of other phenotypic traits or physiological functions is yet to be determined.

The Activation of the CCND1 Promoter by AP-1 and SOX Transcription Factors in PC3 Prostate Cancer Cells Can Be Prevented by Anacardic Acid Analogs.

Targeting more than one in nine men before age 70, prostate cancer is the most common type of cancer in men. The increased levels of cyclins, leading to activation of cyclin-dependent kinases (CDKs), play a critical role in the increased proliferation of prostate cancer cells. In this study, the regulation of the cyclin D1 (CCND1) promoter activity by activator protein-1 (AP-1) and SRY-related HMG-box (SOX) transcription factors has been characterized in PC3 prostate cancer cells.

From molecular to physiological responses: Improved stress tolerance and longevity in Drosophila melanogaster under fluctuating thermal regimes.

Climate change introduces greater thermal variability, profoundly affecting ectothermic species whose body temperatures rely heavily on the environment. Understanding the physiological and metabolic responses to such variability is crucial for predicting how these species will cope with changing climates. This study investigates how chronic thermal stress impacts mitochondrial metabolism and physiological parameters in Drosophila melanogaster, hypothesizing that a fluctuating thermal regime (FTR) activates protective mechanisms enhancing stress tolerance and longevity.

Maintaining Telomeres without Telomerase in : Novel Mechanisms and Rapid Evolution to Save a Genus.

Telomere maintenance is crucial for preventing the linear eukaryotic chromosome ends from being mistaken for DNA double-strand breaks, thereby avoiding chromosome fusions and the loss of genetic material. Unlike most eukaryotes that use telomerase for telomere maintenance, relies on retrotransposable elements-specifically , , and (collectively referred to as HTT)-which are regulated and precisely targeted to chromosome ends. telomere protection is mediated by a set of fast-evolving proteins, termed terminin, which bind to chromosome termini without sequence specificity, balancing DNA damage response factors to avoid erroneous repair mechanisms.

Closing Editorial: Colorectal Cancer-A Molecular Genetics Perspective.

Colorectal cancer (CRC) remains a significant global health challenge, ranking third in incidence and second in mortality among all cancers [...

[Stratégies pour la mise en place d'un chœur intergénérationnel dans une résidence pour personnes aînées afin de contribuer à leur bien-être physique, mental et social].

Dans le contexte pandémique de la COVID-19, les personnes aînées se sont retrouvées confinées et isolées, et ce, même dans le cadre d'un milieu de vie collective. Cet article fait état d'une étude de la portée sur les bienfaits d'un chœur intergénérationnel pour personnes aînées et les stratégies à considérer pour sa mise en place. L'analyse de 16 études sur ce sujet a permis de se familiariser avec les résultats, les recommandations et les pistes de recherche en lien avec le bien-être et le sens de satisfaction que retirent les personnes aînées qui participent à un chœur, de même que des interventions en chant choral à privilégier.

Date palm (Phoenix dactylifera) bioactive constituents and their applications as natural multifunctional ingredients in health-promoting foods and nutraceuticals: A comprehensive review.

Foods that support human health and longevity are becoming increasingly relevant as substitutes for or adjuncts to pharmacological drugs, either through direct consumption or incorporation into designer foods fortified with health-promoting ingredients. Date palm (Phoenix dactylifera L.) fruits, seeds, and pollen are a cornerstone of diverse food and medicine traditions.

A novel energy efficient 4-bit vedic multiplier using modified GDI approach at 32 nm technology.

Multipliers are essential components within digital signal processing, arithmetic operations, and various computational tasks, making their design and optimization crucial for improving the efficiency and performance of integrated circuits. Among multiplier architectures, Vedic multipliers stand out due to their inherent efficiency and speed, derived from ancient Indian mathematical principles. This study presents a comprehensive analysis and comparison of 4-bit Vedic multiplier designs utilizing Gate Diffusion Input (GDI), Complementary Metal-Oxide-Semiconductor (CMOS), and Transmission Gate (TG) technologies, utilizing different adder architectures such as Ripple Carry Adder (RCA), and Carry Lookahead Adder (CLA), Carry Skip Adder (CSA).

The explanatory role of psychological distress in the link between role blurring and relationship satisfaction: A dyadic study.

Role blurring has been associated with negative outcomes, such as anxiety and stress. Paulin et al.'s study found that role blurring is linked to lower relationship satisfaction through higher psychological distress.

Canadian Clinical Guidelines on Social Isolation and Loneliness in Older Adults.

Background: Preventing and addressing social isolation and loneliness among older adults is important because of the known associations with negative health outcomes. The Canadian Coalition for Seniors' Mental Health (CCSMH) took on the task of creating clinical guidelines.

Method: A multidisciplinary working group was established.

Loss of SETD2 in wild-type VHL clear cell renal cell carcinoma sensitizes cells to STF-62247 and leads to DNA damage, cell cycle arrest, and cell death characteristic of pyroptosis.

Loss of chromosome 3p and loss of heterogeneity of the von Hippel-Lindau (VHL) gene are common characteristics of clear cell renal cell carcinoma (ccRCC). Despite frequent mutations on VHL, a fraction of tumors still grows with the expression of wild-type (WT) VHL and evolve into an aggressive subtype. Additionally, mutations on chromatin-modifying genes, such as the gene coding for the histone methyltransferase SET containing domain 2 (SETD2), are essential to ccRCC evolution.

Wolastoqiyik adaptation of the Aaniish Naa Gegii: the Children's Health and Well-Being Measure.

Introduction: Indigenous children in Canada represent one of the fastest-growing pediatric populations and experience severe health inequities. There is an ongoing need for new research on relevant methods to measure the health and wellbeing of Indigenous children that considers the cultural differences between communities. The Aaniish Naa Gegii: the Children's Health and Well-Being Measure (ACHWM) is a self-reported questionnaire that was developed to meet this need and to include the voices of Indigenous children.

The Implementation of the Advocacy Intervention for Midlife and Older Women Who Have Experienced Intimate Partner Violence: Protocol for a Randomized Controlled Trial.

Background: Midlife and older women who experience intimate partner violence (IPV) often have less access to supports and services than younger women. There is far less focus on research and supports for midlife and older women compared to younger women experiencing IPV, and often, neither elder abuse nor IPV services meet their needs. Few interventions are available to meet the needs of midlife and older women.

The impact of patient-facility language discordance on potentially inappropriate prescribing of antipsychotics in long-term care home in Ontario, Canada: a retrospective population health cohort study.

Background: Appropriate use of medication is a key indicator of the quality of care provided in long-term care (LTC). The objective of this study was to determine whether resident-facility language concordance/discordance is associated with the odds of potentially inappropriate prescribing of antipsychotics (PIP-AP) in LTC.

Methods: We conducted a population-based, retrospective cohort study of LTC residents in Ontario, Canada from 2010 to 2019.

Methodological Variations to Explore Conflicting Results in the Existing Literature of Masking Smile Judgment.

Although a smile can serve as an expression of genuine happiness, it can also be generated to conceal negative emotions. The traces of negative emotion present in these types of smiles can produce micro-expressions, subtle movements of the facial muscles manifested in the upper or lower half of the face. Studies examining the judgment of smiles masking negative emotions have mostly employed dichotomous rating measures, while also assuming that dichotomous categorization of a smile as happy or not is synonymous with judgments of the smile's authenticity.

The Interaction Between the Production Effect and Serial Position in Recognition and Recall.

In memory tasks, items read aloud are better remembered than their silently read counterparts. This production effect is often interpreted by assuming a distinctiveness benefit for produced items, but whether this benefit also comes at a cost remains up for debate. In recall tasks, when pure lists are used in which all items are produced or read silently, studies have shown a better recall of produced items at the last serial positions, but a lower recall at the first positions.

Hospital Re-Admission Prediction Using Named Entity Recognition and Explainable Machine Learning.

Early hospital readmission refers to unplanned emergency admission of patients within 30 days of discharge. Predicting early readmission risk before discharge can help to reduce the cost of readmissions for hospitals and decrease the death rate for Intensive Care Unit patients. In this paper, we propose a novel approach for prediction of unplanned hospital readmissions using discharge notes from the MIMIC-III database.

Exploration of primary care models and timely access to care in New Brunswick (Canada).

Background: This correlative study aimed to examine how the different primary care models (physicians in solo practice, physicians in collaborative practice, physicians and nurse practitioners in collaborative practice, after-hours clinics, community centers, or emergency rooms) were associated with their capability to offer timely access to their patients. The data collected from the primary care provider's perspective was to complete the New Brunswick Health Council results on patients' perspective.

Methods: A convenience sample of 120 primary care providers (33 physicians in solo practice, 33 physicians in collaborative practice, 27 providers in collaborative practice with nurse practitioners, 2 providers working in after-hours clinics, and 10 providers in Emergency departments) responded to an online survey about their primary care models and accessibility.