Urbanization enhances consumer protist-driven ARGs dissemination in riverine ecosystems.

Despite the emergence of antibiotic resistance genes (ARGs) and antibiotic resistant bacteria (ARBs), how biological inter-trophic interactions, modulated by watershed urbanization, shape the resistome remains unexplored. We collected water samples from the highly urbanized (western: 65 % built land, sewage-affected) and lesser-urbanized (northern: 25 % built land, drinking water source) downstream tributaries of the Jiulong River in southeast China over dry and wet seasons. We utilized metagenomic and amplicon (16S and 18S rDNA) sequencing to investigate the relationships among microeukaryotic algae, consumer protists, bacterial communities, and the resistome.

Valuation of marine integrated disaster management amidst global warming in Southern Coast of Java, Indonesia.

This research explores the valuation of integrated disaster management in the coastal regions of Southern Java, Indonesia, a locale increasingly threatened by the impacts of global warming, which exacerbates marine disasters such as earthquakes and tsunamis. Employing a choice experiment methodology, the study assesses the willingness to pay among local households for various strategies designed to enhance earthquake preparedness. Three distinct scenarios are examined, each reflecting varying levels of integration and sophistication: (1) Educational empowerment and localized alert integration, which emphasizes community education and rapid, self-directed evacuation practices tailored to the immediate onset of tsunamis following seismic events.

Natural Compounds and Their Analogs as Antivirals Against Dengue Virus: A Review.

Dengue virus (DENV) continues to pose a significant global health challenge, causing diseases such as dengue fever, dengue hemorrhagic fever, and dengue shock syndrome. While efforts in vaccine development and antiviral drug discovery are ongoing, effective therapeutic options remain limited. In this review, we highlight natural compounds and the analogs that demonstrated antiviral activity against DENV in in vitro and in vivo studies.

Unveiling the critical role of overlooked consumer protist-bacteria interactions in antibiotic resistance gene dissemination in urban sewage systems.

Antibiotic resistance genes (ARGs) are emerging contaminants of significant concern due to their role in facilitating the spread of antibiotic resistance, especially high-risk ARGs, which are characterized by high human accessibility, gene mobility, pathogenicity, and clinical availability. Studies have shown that cross-domain interactions, such as those between consumer protists (consumers) and bacteria, can influence bacterial diversity, distribution, and function through top-down control. The consumers-bacteria interactions may also affect the occurrence and distribution of ARGs, yet this has been scarcely explored in field investigations.

Water pollution and sanitation in Indonesia: a review on water quality, health and environmental impacts, management, and future challenges.

As an archipelagic tropical developing country, Indonesia is characterized by vast expanses of rural and isolated areas. This review aims to discuss water pollution and sanitation's existing condition, health, and environmental impacts, alongside its management and challenges in Indonesia. The systematic review approach was utilized to ensure transparency and replicability, following the Preferred Reporting Items for Systematic Reviews and Meta-Analyses (PRISMA) criteria.

Boosting beach clean-up participation through community resilience hypothetical scenarios.

Engaging coastal communities in beach clean-up initiatives is crucial for the sustainability of marine ecosystems and the support of the blue economy. This study aims to examine the influence of various resilience attributes on community willingness to participate in these activities. It uses choice experiment framework to test three distinct scenarios: (1) cultural recycling education, (2) social media task force, and (3) resilience debris recovery.

Leveraging protein language model embeddings and logistic regression for efficient and accurate in-silico acidophilic proteins classification.

The increasing demand for eco-friendly technologies in biotechnology necessitates effective and sustainable catalysts. Acidophilic proteins, functioning optimally in highly acidic environments, hold immense promise for various applications, including food production, biofuels, and bioremediation. However, limited knowledge about these proteins hinders their exploration.

Evaluating factors influencing community readiness for post-mining environmental development strategies.

This research delves into the complex factors that affect how ready a community is to embrace new environmental development strategies, which could significantly change the region's social and economic fabric. Using a structured questionnaire, exploratory factor analysis and logistic regression analysis, the study assesses how corporate practices in resource management, environmental governance, efforts to enhance community capabilities, and various demographic factors influence the community's willingness to adapt to change. Despite its intentions to benefit both the mining operations and the community, corporate resource management appears to have a paradoxical impact on the community's willingness to pursue new environmental paths.

PaCS-Toolkit: Optimized Software Utilities for Parallel Cascade Selection Molecular Dynamics (PaCS-MD) Simulations and Subsequent Analyses.

Parallel cascade selection molecular dynamics (PaCS-MD) is an enhanced conformational sampling method conducted as a "repetition of time leaps in parallel worlds", comprising cycles of multiple molecular dynamics (MD) simulations performed in parallel and selection of the initial structures of MDs for the next cycle. We developed PaCS-Toolkit, an optimized software utility enabling the use of different MD software and trajectory analysis tools to facilitate the execution of the PaCS-MD simulation and analyze the obtained trajectories, including the preparation for the subsequent construction of the Markov state model. PaCS-Toolkit is coded with Python, is compatible with various computing environments, and allows for easy customization by editing the configuration file and specifying the MD software and analysis tools to be used.

Classifying alkaliphilic proteins using embeddings from protein language model.

Alkaliphilic proteins have great potential as biocatalysts in biotechnology, especially for enzyme engineering. Extensive research has focused on exploring the enzymatic potential of alkaliphiles and characterizing alkaliphilic proteins. However, the current method employed for identifying these proteins that requires web lab experiment is time-consuming, labor-intensive, and expensive.

Chemisorption of silicon tetrachloride on silicon nitride: a density functional theory study.

We studied the chemisorption of silicon tetrachloride (SiCl) on the NH/NH-terminated silicon nitride slab model using density functional theory (DFT) for atomic layer deposition (ALD) of silicon nitride. Initially, two reaction pathways were compared, forming HCl or NHCl as a byproduct. The NHCl complex formation was more exothermic than the HCl formation, with an activation energy of 0.

An ultra-sensitive SARS-CoV-2 antigen optical biosensor based on angiotensin converting enzyme 2 (ACE-2) functionalized magnetic-fluorescent silica nanoparticles.

This work reports on the design and synthesis of an angiotensin-converting enzyme 2 (ACE-2) functionalized magnetic fluorescent silica nanoparticles (Fe-FSNP) as a biosensing platform to detect severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) antigen. Iron oxide (FeO) nanoparticles were synthesized via ultrasonic-assisted coprecipitation and then coated with fluorescent silica nanoparticles (FSNP) through themethod forming the Fe-FSNP samples. Silica obtained from local geothermal powerplant was used in this work and Rhodamine B was chosen as the incorporated fluorescent dye, hence this reports for the first time ACE-2 was immobilized on the natural silica surface.

Mass-immigration shapes the antibiotic resistome of wastewater treatment plants.

Wastewater treatment plants (WWTPs) are the hotspots for the spread of antibiotic resistance genes (ARGs) into the environment. Nevertheless, a comprehensive assessment of the city-level and short-term daily (inter-day) variations of ARG profiles in the whole process (influent-INF, activated sludge-AS and effluent-EF) of WWTPs is still lacking. Here, 285 ARGs and ten mobile gene elements were monitored in seven WWTPs in Xiamen for seven days via high-throughput qPCR.

Effect of Operating Parameters on the Performance of Integrated Fixed-Film Activated Sludge for Wastewater Treatment.

Integrated fixed-film activated sludge (IFAS) is a hybrid wastewater treatment process that combines suspended and attached growth. The current review provides an overview of the effect of operating parameters on the performance of IFAS and their implications for wastewater treatment. The operating parameters examined include hydraulic retention time (HRT), solids retention time (SRT), dissolved oxygen (DO) levels, temperature, nutrient loading rates, and aeration.

A theoretical study on the surface reaction of tetrakis(dimethylamino)titanium on titanium oxide.

Tetrakis(dimethylamino)-titanium (TDMAT, Ti(NMe)) has been used for the low-temperature atomic layer deposition (ALD) process of titanium oxide (TiO) films. In this study, the chemisorption of TDMAT on a titanium oxide surface using a slab model was simulated by density functional theory (DFT) calculation. We calculated the activation energy for the chemisorption and predicted the final chemisorbed species.

Energetic and Kinetic Origins of CALB Interfacial Activation Revealed by PaCS-MD/MSM.

The conformational dynamics of lipase B (CALB) was investigated by molecular dynamics (MD) simulation, parallel cascade selection MD (PaCS-MD), and the Markov state model (MSM) and mainly focused on the lid-opening motion closely related to substrate binding. All-atom MD simulation of CALB was conducted in water and on the interface of water and tricaprylin. CALB initially situated in water and separated by layers of water from the interface is spontaneously adsorbed onto the tricaprylin surface during MD simulation.

Quantum mechanical assessment on the optical properties of capsanthin conformers.

As optical properties, the ultraviolet-visible (UV-Vis) absorption spectra of capsanthin-based red natural dye are a decisive parameter for their usage in various applications. Thus, accurately predicting the maximum UV-Vis wavelength ( ) values is critical in designing dye-conjugated material. Extensive metadynamics simulations were carried out to generate capsanthin conformers at various levels of the extended tight-binding method.

Recent advances in synthesis of polymers based on palm oil and its fatty acids.

Palm oil is a versatile bio-renewable resource for consumer products, oleochemicals, and biofuels. The utilization of palm oil in polymer production as a bio-based polymer is considered a promising alternative to conventional petrochemical-based polymers due to its non-toxicity, biodegradability, and vast obtainability. Triglycerides and fatty acids in palm oil and their derivatives can be utilized as bio-based monomers for synthesizing polymers.

Urbanization influenced the interactions between dissolved organic matter and bacterial communities in rivers.

Increased urbanization and anthropogenic activities can alter dissolved organic matter (DOM) and complicate its interaction with bacteria in rivers' ecosystems, however, there is limited information about how bacterial communities respond to DOM components in rivers with different urbanization levels. Here, we combined spectroscopy-based DOM analysis and 16S rRNA gene amplicon sequencing to investigate the associations of bacterial taxa and DOM properties as well as the impacts of DOM on bacterial niche breadth in North River (NR) and West River (WR) of Jiulong River watershed, southern China, which had low and high urbanization levels, respectively. Spectroscopy analysis showed that hydrophilic DOM was predominant in both rivers whereas chromophoric DOM was higher in WR.

Nylon 6,6 Waste Nanofiber Membrane for Produced Water Filtration: Experimental, Performance Modelling, Optimization and Techno-Economic Analysis.

Produced water (PW) is a by-product of oil and gas extraction, of which it is deemed as the primary contributor of wastewater stream in oil production. Conventional treatment such as membrane separation is favoured due to its sustainability and cost effectiveness. On the other hand, oceanic litters such as abandoned fishing nets endangered the marine life ecosystem, despite of its potential to be raw material for fabrication of nanofiber membrane (NFM).

Selective etching mechanism of silicon oxide against silicon by hydrogen fluoride: a density functional theory study.

Selective etching of silicon oxide (SiO) against silicon (Si) using anhydrous hydrogen fluoride (HF) vapor has been used for semiconductor device fabrication. We studied the underlying mechanism of the selective etching by density functional theory (DFT) calculation. We constructed surface slab models of SiO or Si with different degrees of fluorination and simulated the four steps of fluorination.

Fabrication of hybrid covalent triazine framework-zinc ferrite spinel to uplift visible light-driven photocatalytic organic pollutant degradation.

The tunability of porous covalent triazine frameworks (CTFs) can mitigate poor photostability and rapid hole-electron recombination. Herein, an excellent improvement of visible light-driven photocatalytic pollutant degradation was achieved using a hybrid semiconductor of covalent triazine framework-zinc ferrite spinel catalysts (CTF-ZnFeO). The as-prepared CTF-ZnFeO composites were fabricated using a facile one-pot ionothermal method.

Characterization of thermostable serine hydroxymethyltransferase for β-hydroxy amino acids synthesis.

β-hydroxy amino acids, such as serine, threonine, and phenylserine, are important compounds for medical purposes. To date, there has been only limited exploration of thermostable serine hydroxylmethyltransferase (SHMT) for the synthesis of these amino acids, despite the great potential that thermostable enzymes may offer for commercial use due to their high stability and catalytic efficiencies. ITBSHMT_1 (ITB serine hydroxylmethyltransferase clone number 1) from thermophilic and methanol-tolerant bacteria Pseudoxanthomonas taiwanensis AL17 was successfully cloned.

Seasonal hydrological dynamics govern lifestyle preference of aquatic antibiotic resistome.

Antibiotic resistance genes (ARGs) are a well-known environmental concern. Yet, limited knowledge exists on the fate and transport of ARGs in deep freshwater reservoirs experiencing seasonal hydrological changes, especially in the context of particle-attached (PA) and free-living (FL) lifestyles. Here, the ARG profiles were examined using high-throughput quantitative PCR in PA and FL lifestyles during four seasons representing two hydrological phenomena (vertical mixing and thermal stratification) in the Shuikou Reservoir (SR), Southern China.