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Production of vasoactive substances by human umbilical vein endothelial cells after incubation with serum from preeclamptic patients.
Eur J Obstet Gynecol Reprod Biol
December 2001
Department of Obstetrics and Gynecology, University of Erlangen/Nuremberg, Universitaetsstr. 2-23, 91054, Erlangen, Germany.
Objective: To determine the in vitro effect of serum from preeclamptic patients on the proliferation, viability and secretion of vasoactive substances by human umbilical vein endothelial cells (HUVEC).
Study Design: HUVEC were incubated for 24h with sera from 16 preeclamptic, 19 healthy pregnant and 8 healthy nonpregnant women. Proliferation rates were determined by cell counting and vitality by trypan blue staining.