Handling of gliadin peptides B1-B4 and of cow's milk proteins by rat jejunum gut sacs.

The rat everted gut sac was used as a model of intestinal protein and peptide handling to study small intestinal binding and uptake of different gliadin peptide fractions (B1, B2, B3, B4) in comparison with bovine serum albumin (BSA), alpha-lactalbumin (alpha-LA), and a BSA peptide fraction (BSA-P). Not only binding curves, but also uptake curves of BSA, alpha-LA, and BSA-P were run in parallel between 1 and 30 min. Binding and uptake obviously depended on molecular weight.