Dendritic cells as stimulator cells of MHC class I-restricted immune responses.

We have shown that growth factor-dependent, MHC class I+/II dendritic cell lines established from mouse fetal skin, can stimulate naive, allogeneic but not syngeneic CD8+ T cells in the absence of CD4+ T cells and that this T cell response is restricted by MHC class I molecules. We further showed that the FSCL-induced activation of naive CD8+ T cells is critically dependent on the physical contact between stimulator and responder cells and the expression of the costimulatory molecule B7 on FSCL. An important question that remains to be addressed concerns the derivation of FSCL.