Humanized Candida and NanoBiT Assays Expedite Discovery of Bdf1 Bromodomain Inhibitors With Antifungal Potential.

The fungal Bromodomain and Extra-Terminal (BET) protein Bdf1 is a potential antifungal target against invasive fungal infections. However, the need to selectively inhibit both Bdf1 bromodomains (BDs) over human orthologs and the lack of molecular tools to assess on-target antifungal efficacy hamper efforts to develop Bdf1 BD inhibitors as antifungal therapeutics. This study reports a phenyltriazine compound that inhibits both Bdf1 BDs from the human fungal pathogen Candida glabrata with selectivity over the orthologous BDs from the human BET protein Brd4.

Computerized cognitive training for problem gambling: A randomized controlled trial (TRAIN-online).

Background: Non-face-to-face interventions offer promise, with cognitive training showing potential but inconsistent efficacy in problem gambling.

Methods: We conducted a non-face-to-face, parallel, randomized, controlled, single-blinded trial to evaluate training programs in adults with problem gambling (CPGI ≥5). Participants were randomized 1:1 to a web-based cognitive training program targeting inhibition unrelated to addiction cues or a control program on visuo-spatial functioning.

Off-axis electron holography of unstained bacteriophages: Toward electrostatic potential measurement of biological samples.

Transmission electron microscopy, especially at cryogenic temperature, is largely used for studying biological macromolecular complexes. A main difficulty of TEM imaging of biological samples is the weak amplitude contrasts due to electron diffusion on light elements that compose biological organisms. Achieving high-resolution reconstructions implies therefore the acquisition of a huge number of TEM micrographs followed by a time-consuming image analysis.

Writing and drawing tilts after right hemisphere stroke are signs of a wrong verticality representation.

Background: Many signs of spatial dysgraphia and drawing errors after right hemispheric stroke (RHS) have been attributed to spatial neglect or impaired sensory feedback. Counterclockwise (contralesional) tilts of graphomotor productions remained to be explained.

Objective: To test whether graphomotor tilts stem from a tilted representation of verticality transposed to the top/bottom axis of the sheet of paper, using data from the DOBRAS cohort.

Calcium oxalate crystals in cacao trees and their interactions with cadmium.

Cadmium (Cd) concentrations in cacao beans from Latin America often exceed limits for trading. A better understanding of the mechanisms of Cd accumulation in Theobroma cacao L. trees is necessary to advance mitigation strategies.

Optimizing work in community pharmacy: What preferences do community pharmacists and pharmacy technicians have for a better allocation of daily activities?

Objective: Pharmacy work encompasses two main streams. These are logistic flow (the supply and distribution of healthcare products) and pharmaceutical flow (the dispensing and provision of pharmacy services). The pharmaceutical flow has increased significantly with the introduction of reimbursed services such as Rapid Diagnostic Tests, chronic disease screening, minor ailment prescriptions, vaccine prescription and administration, and medication reviews.

Time course of hospitalizations in patients with heart failure and chronic obstructive pulmonary disease around sleep-disordered-breathing diagnosis.

Purpose: In heart failure (HF) and chronic obstructive pulmonary disease (COPD) populations, sleep-disordered breathing (SDB) is associated with impaired health outcomes. We evaluated whether in patients with HF, concomitant HF and COPD or COPD, the number of hospitalizations would be reduced in the year after testing for SDB with and without treatment initiation compared to the year before.

Methods: We performed a multicentre retrospective study of 390 consecutive sleep-clinic patients who had a primary diagnosis of chronic HF, HF and COPD or COPD and a secondary diagnosis of SDB.

Use of assistive technology to assess distal motor function in subjects with neuromuscular disease.

Unlabelled: Among the 32 items of the Motor Function Measure scale, 3 concern the assessment of hand function on a paper-based support. Their characteristics make it possible to envisage the use of a tablet instead of the original paper-based support for their completion. This would then make it possible to automate the score to reduce intra- and inter-individual variability.

An open source ultrasonic flowmeter for monitoring the input/output flow rates of wastewater treatment plants.

Sensors play an important role in both the continuous monitoring and intermittent analyses, which are essential for the study of wastewater treatment plant management and conducting related research. Given the significant environmental impact of the issues involved, accurate measurement of the volume of water flowing into and out of treatment plants is a key parameter for plant management, ecotoxicological studies and academic research programs. Traditionally, flow measurements have been based on calibrated weirs or venturi flumes, using water level measurements for conversion into flow, according to established relationships.

First-Principles Assessment of ZnTe and CdSe as Prospective Tunnel Barriers at the InAs/Al Interface.

Majorana zero modes are predicted to emerge in semiconductor/superconductor interfaces, such as InAs/Al. Majorana modes could be utilized for fault tolerant topological qubits. However, their realization is hindered by materials challenges.

Betaine lipids: Biosynthesis, functional diversity and evolutionary perspectives.

Betaine lipids (BL) are relatively understudied non‑phosphorus glycerolipids. They are predominantly found in algae but have also been detected in other unicellular eukaryotes, fungi, bacteria, and some bryophytes and pteridophytes. These extraplastidial lipids are considered as substitute for phospholipids in organisms, particularly under phosphate (Pi) deficiency.

Relations between psychosis and emotion regulation in daily life: A systematic review and meta-analyses of studies using experience sampling methods.

Research has shown that heightened emotionality often precedes psychotic experiences. Understanding how individuals cope with these emotions is crucial for psychosis. While prior studies on emotion regulation (ER) and psychosis have mainly relied on self-report questionnaires, recent research has increasingly utilised daily life methods.

Nanostructured lipid carriers based mRNA vaccine leads to a T cell-inflamed tumour microenvironment favourable for improving PD-1/PD-L1 blocking therapy and long-term immunity in a cold tumour model.

Background: mRNA-based cancer vaccines show promise in triggering antitumour immune responses. To combine them with existing immunotherapies, the intratumoral immune microenvironment needs to be deeply characterised. Here, we test nanostructured lipid carriers (NLCs), the so-called Lipidots®, for delivering unmodified mRNA encoding Ovalbumin (OVA) antigen to elicit specific antitumour responses.

Biliary diffusion and antifungal activity of caspofungin and fluconazole in liver transplant recipients: A pilot study.

Invasive candidiasis, including intra-abdominal candidiasis (IAC), is a common complication after liver transplantation. Antifungal drugs such as echinocandins and fluconazole (FCZ) are frequently used to prevent or treat such fungal infections. The diffusion of these antifungals within abdominal body sites has been rarely reported, in particular, in liver transplant recipients.

Neuronal Imaging at 8-Bit Depth to Combine High Spatial and High Temporal Resolution With Acquisition Rates Up To 40 kHz.

A challenge in neuroimaging is acquiring frame sequences at high temporal resolution from the largest possible number of pixels. Measuring 1%-10% fluorescence changes normally requires 12-bit or higher bit depth, constraining the frame size allowing imaging in the kHz range. We resolved Ca or membrane potential signals from cell populations or single neurons in brain slices by acquiring fluorescence at 8-bit depth and by binning pixels offline, achieving unprecedented frame sizes at kHz rates.

α/β hydrolase domain-containing protein 1 acts as a lysolipid lipase and is involved in lipid droplet formation.

Lipid droplets (LDs) are the major sites of lipid and energy homeostasis. However, few LD biogenesis proteins have been identified. Using model microalga , we show that ABHD1, an α/β-hydrolase domain-containing protein, is localized to the LD surface and stimulates LD formation through two actions: one enzymatic and one structural.

Pacmanvirus isolated from the Lost City hydrothermal field extends the concept of transpoviron beyond the family Mimiviridae.

The microbial sampling of submarine hydrothermal vents remains challenging, with even fewer studies focused on viruses. Here we report the first isolation of a eukaryotic virus from the Lost City hydrothermal field, by co-culture with the laboratory host Acanthamoeba castellanii. This virus, named pacmanvirus lostcity, is closely related to previously isolated pacmanviruses (strains A23 and S19), clustering in a divergent clade within the long-established family Asfarviridae.

Transfer of motor and strategy learning in children with Developmental Coordination Disorder (DCD): A scoping review.

Developmental coordination disorder (DCD) is a developmental disorder that affects the learning and execution of motor skills. Little is known about their ability to transfer their learning, i.e.

Intertwined people-nature relations are central to nature-based adaptation to climate change.

Adaptation to climate change is a social-ecological process: it is not solely a result of natural processes or human decisions but emerges from multiple relations within social systems, within ecological systems and between them. We propose a novel analytical framework to evaluate social-ecological relations in nature-based adaptation, encompassing social (people-people), ecological (nature-nature) and social-ecological (people-nature) relations. Applying this framework to 25 case studies, we analyse the associations among these relations and identify archetypes of social-ecological adaptation.

Investigating Parkinson's disease risk across farming activities using data mining and large-scale administrative health data.

The risk of Parkinson's disease (PD) associated with farming has received considerable attention, in particular for pesticide exposure. However, data on PD risk associated with specific farming activities is lacking. We aimed to explore whether specific farming activities exhibited a higher risk of PD than others among the entire French farm manager (FM) population.

Visual attention matters during word recognition: A Bayesian modeling approach.

It is striking that visual attention, the process by which attentional resources are allocated in the visual field so as to locally enhance visual perception, is a pervasive component of models of eye movements in reading, but is seldom considered in models of isolated word recognition. We describe BRAID, a new Bayesian word-Recognition model with Attention, Interference and Dynamics. As most of its predecessors, BRAID incorporates three sensory, perceptual, and orthographic knowledge layers together with a lexical membership submodel.

Bathymetry of the Antarctic continental shelf and ice shelf cavities from circumpolar gravity anomalies and other data.

Bathymetry critically influences the intrusion of warm Circumpolar Deep Water onto the continental shelf and under ice shelf cavities in Antarctica, thereby forcing ice melting, grounding line retreat, and sea level rise. We present a novel and comprehensive bathymetry of Antarctica that includes all ice shelf cavities and previously unmeasured continental shelf areas. The new bathymetry is based on a 3D inversion of a circumpolar compilation of gravity anomalies constrained by measurements from the International Bathymetric Chart of the Southern Ocean, BedMachine Antarctica, and discrete seafloor measurements from seismic and ocean robotic probes.

Advanced Analysis of Clay Microporosity Using High-Resolution N-Ar Adsorption Isotherms Coupled with the Derivative Isotherm Summation Method.

The textural properties of synthetic and natural clays in the sodium form and exchanged with tetramethylammonium cations (TMA) were characterized using N and Ar physisorption isotherms at cryogenic temperatures. Specific surface areas and micro/mesoporous volumes were determined using the BET and the models. The analysis requires the use of reference isotherms measured at the same temperature on the surface of non-porous materials with an identical chemical composition.

Faithful interpretation of protein structures through weighted persistent homology improves evolutionary distance estimation.

Phylogenetic inference is mainly based on sequence analysis and requires reliable alignments. This can be challenging, especially when sequences are highly divergent. In this context, the use of three-dimensional protein structures is a promising alternative.