Health care institutions and their physicians are the greatest promoters of COVID-19 vaccine acceptance among health care workers.

Background: ESPERES, a prospective e-cohort study is designed to assess attitudes and perceptions of the COVID-19 pandemic among French health care workers.

Objectives: We aimed to document (i) their confidence in the information directly transmitted by their health care institutions; (ii) the role of physicians in the development of normative beliefs about vaccines against COVID-19 among them; (iii) their view of issues related to governmental health institution communication; and (iv) the impact of this communication on their posture to promote vaccine adherence among patients.

Methods: We conducted semistructured interviews with health care workers from the ESPERES cohort (n = 50).

Opportunities and challenges for the use of human samples in translational cardiovascular research: a scientific statement of the ESC Working Group on Cellular Biology of the Heart, the ESC Working Group on Cardiovascular Surgery, the ESC Council on Basic Cardiovascular Science, the ESC Scientists of Tomorrow, the European Association of Percutaneous Cardiovascular Interventions of the ESC, and the Heart Failure Association of the ESC.

Animal models offer invaluable insights into disease mechanisms but cannot entirely mimic the variability and heterogeneity of human populations, nor the increasing prevalence of multi-morbidity. Consequently, employing human samples-such as whole blood or fractions, valvular and vascular tissues, myocardium, pericardium, or human-derived cells-is essential for enhancing the translational relevance of cardiovascular research. For instance, myocardial tissue slices, which preserve crucial structural and functional characteristics of the human heart, can be used in vitro to examine drug responses.

Comparable outcomes between cruciate-substituting and posterior-stabilized inserts in robotic total knee arthroplasty under the functional alignment principles.

Purpose: Functional alignment (FA) has emerged as a personalized strategy in total knee arthroplasty (TKA) to optimize outcomes by accounting for patient-specific anatomical and soft tissue characteristics. Limited evidence exists on how polyethylene insert type, specifically cruciate-substituting (CS) versus posterior-stabilized (PS), impacts clinical outcomes and complications in this context.

Methods: This retrospective comparative study included 329 patients who underwent robotic-assisted TKA with FA principles with a minimum 2-year follow-up.

In Vitro Activity of Bacteriophages Against Ocular Methicillin-resistant S. aureus Isolates Collected in the US.

Introduction: Methicillin-resistant Staphylococcus aureus (MRSA) is a leading cause of sight-threatening infections in the US. These strains pose a significant challenge in managing ocular infections, as they frequently exhibit resistance to first-line empirical antibiotics. To assess the potential of bacteriophages as innovative topical therapies for treatment of recalcitrant ocular infections, we evaluated the in vitro antimicrobial activity of a set of anti-S.

Retrovirus-Mediated Reprogramming of Endogenous Hippocampal Glia into GABAergic Induced Neurons.

Lineage reprogramming of glial cells into induced neurons (iNs) has emerged as an innovative strategy to replace neurons lost due to injury or neurological diseases. Here, we describe a step-by-step protocol to induce in vivo conversion of reactive glial cells, proliferating within the injured hippocampus, into mature and functional GABAergic iNs through retrovirus-mediated expression of two neurogenic fate determinants (Ascl1 and Dlx2). We have previously applied this method to study the integration and functional impact of GABAergic iNs in epileptic mice (Lentini et al.

High-Dose Vitamin D in Clinically Isolated Syndrome Typical of Multiple Sclerosis: The D-Lay MS Randomized Clinical Trial.

Importance: Vitamin D deficiency is a risk factor for multiple sclerosis (MS) and is associated with the risk of disease activity, but data on the benefits of supplementation are conflicting.

Objective: To evaluate the efficacy of high-dose cholecalciferol as monotherapy in reducing disease activity in patients with clinically isolated syndrome (CIS) typical for MS.

Design, Setting, And Participants: The D-Lay MS trial was a parallel, double-blind, randomized placebo-controlled clinical trial in 36 MS centers in France.

[Deciphering the links between hepatic viruses and interferons: molecular interactions and therapeutic implications].

Interferons (IFNs) are cytokines that play a key role in the innate immune response, possessing antiviral, antiproliferative, and immunomodulatory properties. Currently, IFNs are used clinically as treatments for lymphomas, multiple sclerosis, and chronic viral infections such as hepatitis B, C, D, and E virus infections. HEV infection can lead to fulminant hepatitis, while chronic infections with HBV, HCV, and HDV are the leading global causes of hepatocellular carcinoma.

ABOV: A Novel System of Direct Headspace Skin Sampling to Study Human Body Odor.

Chemicals emitted by the human body convey information about the individuals. However, our understanding of the chemical underpinnings of human chemical communication remains limited, partly due to methodological constraints. Here, we describe a novel sampling technique, named ABOV (Analysis of Body Odor Volatiles), for analyzing the chemical composition of human skin odor.

Microplastics storage at the sediment-water interface in a gravel-bed river: Importance of local hydro-sedimentary conditions in downwelling, upwelling, and sedimentation zones.

Rivers are often seen as vectors for microplastic transport from land to the ocean; however, the sediment-water interface of rivers also provides opportunity for the temporary storage and retention of plastic debris. Hydrological, sedimentary, physical, chemical and biological factors influence microplastic fate and transport, yet the influence of the interactions between these different drivers and mechanisms on microplastic transport at the sediment-water interface remains poorly understood. This study investigates how hydrological exchanges impact microplastics distribution in streambed sediments by comparing microplastic concentrations in zones with surface-groundwater exchange fluxes (upwelling or downwelling) and zones with sediment accumulation along gravel bars situated in the lower catchment of the Ain River (France).

An ageing study of twenty 18650 lithium-ion Graphite/LFP cells in first and second life use.

The dataset gives a comprehensive data collection focusing on the ageing of twenty 18650 Graphite/LFP 1.1 Ah commercial cells in first and second life. The ageing from a pristine state up to 40% of capacity loss is uncommon due to the extensive testing time required.

Cerebral blood flow in Andean children and adolescents living above 5000m.

A number of indigenous populations have resided at high-altitude for generations, resulting in various phenotypical adaptations promoting successful high-altitude adaptation. While many of these adaptations have been investigated in adults, little is known regarding how children residing at high-altitudes adapt, particularly with regards to the cerebrovasculature. Under hypoxic environments, compensatory changes in cerebral blood flow (CBF) are necessary to couple oxygen delivery to metabolic demand in the face of reduced oxygen availability.

Asymptotic Underscreening in Concentrated Electrolytes Measured by Optical Second Harmonic Scattering of Water.

We show that with optical second harmonic scattering, one can measure the screening length in the bulk phase of aqueous electrolytes. The methodology is based on the analysis of the coherent contribution describing the water-water orientation correlation function present in the polarization-resolved optical second harmonic scattering intensity. Measurements were performed up to highly concentrated NaCl and LiCl electrolyte solutions, enabling the development of a theoretical framework valid over the whole salt concentration range.

Host-targeted repurposed diltiazem enhances the antiviral activity of direct acting antivirals against Influenza A virus and SARS-CoV-2.

Viral respiratory infections remain a major and recurrent public health threat. Among them, influenza viruses are responsible for ⁓500,000 deaths worldwide and a high economic burden. The recurrent threat of emerging zoonotic or pandemic viruses worsens this scenario, being SARS-CoV-2 and the millions of COVID-19 deaths the most recent example.

Eyring theory for plasticity in amorphous polymers violates Curie's principle.

In 1936, Eyring introduced a model for plastic flow which still forms the bedrock of practically all studies on plasticity of glassy polymers. Though the concept of activated process he introduced is fundamentally relevant, we argue here that under no circumstances can the Eyring model be correct as it violates Curie's principle, which is a basic physical requirement of statistical mechanics. An alternative model was proposed by [Long , , 2018, , 105601] to describe the acceleration of the dynamics by an applied stress, in which the elastic energy stored at the length scale of dynamical heterogeneities ≈ 3 - 5 nm reduces the free energy barriers for relaxation.

Serotonergic dysfunction in patients with impulse control disorders in Parkinson's disease.

Impulse control disorders (ICDs) are frequent and particularly distressing neuropsychiatric symptoms in patients with Parkinson's disease (PD) which are related to impaired behavioural inhibition. Multiple PET imaging studies indicate that striatal dopaminergic abnormalities contribute to hyperdopaminergic functioning in PD patients with ICD (PDICD+) and to the dysregulation of the limbic fronto-striatal networks which are critical for reward-related decision impulsivity. However, the serotonergic system is central to response inhibition and plays a critical role in neuropsychiatric symptoms in PD, but its role remains undetermined in PDICD.

Metastatic Pure Seminomas With Early Relapse: Prognostic Roles of High Dose Chemotherapy and Surgery of Residual Disease.

Introduction: Metastatic seminomatous germ cell tumors (mSGCT) are a rare form of cancer. Few studies focused on early relapse (<12 months) after first-line chemotherapy (ChT). We aimed to retrospectively evaluate the impact of salvage retroperitoneal lymph node dissection (RPLND) and high-dose ChT with hematopoietic stem cell transplantation (HDCT-HSCT) in mSGCT patients in a situation of early relapse.

Cytokine Signature Unveils Subgroups of Patients With Immune-Mediated Sensory Neuronopathies.

Background And Aims: Immune-mediated sensory neuronopathies (SNN) can occur alongside autoimmune disorders (e.g., Sjögren syndrome), involve autoantibodies (such as anti-FGFR3 or anti-AGO antibodies), or present in isolation.

An international observational study validating gene-expression sepsis immune subgroups.

Background: Sepsis gene-expression sub-phenotypes with prognostic and theranostic potential have been discovered. These have been identified retrospectively and have not been translated to methods that could be deployed at the bedside. We aimed to identify subgroups of septic patients at high-risk of poor outcome, using a rapid, multiplex RNA-based test.

An LNP-mRNA vaccine protects fish against rhabdovirus infection.

mRNA vaccines are poised to revolutionize disease prevention, following the approval of their administration to humans against SARS-CoV-2. Although they have been extensively studied for human applications, their potential in the veterinary field has not been explored yet. No mRNA vaccines have yet been reported for fish, despite the urgent need for new vaccines against emerging pathogens in aquaculture.

The antibody repertoire of autoimmune sensory neuronopathies targets pathways of the innate and adaptive immune system. An autoantigenomic approach.

Sensory neuronopathies (SNN) encompass diverse etiologies, with autoimmunity playing a major role through both cellular and humoral responses. To investigate the humoral autoantibody repertoire in autoimmune SNN, we conducted a retrospective cohort study using large Human Proteome-wide protein microarrays (HuProt 3.1, HuProt 4.

Comparing the capacity of intraoral scanner and expert scoring in detecting potential deviations in guided endodontics: an in vitro study.

Introduction: Guided endodontics recently offers a conservative solution for conducting root canal treatment in cases of obliterated canals, but guide mispositioning remains a challenge. Recently, intraoral scanners (IOS) were proposed to detect deviations, but their effectiveness compared to expert visual assessments during guide insertion is uncertain. The objective of the present study was to primarily compare the risk of deviation predicted by an IOS versus expert assessment and secondarily identify factors influencing this risk.

Human cortical bone intrinsic permeability distribution based on 3D canalicular morphology.

Bone permeability is a key parameter that drives osteocyte-based mechanobiological modelling and remodelling. While previous experimental and numerical studies have estimated bone permeability based on the morphology of the lacuno-canalicular network, these studies often relied on simplified geometries. In the current study, bone permeability was characterized using more realistic canalicular geometry for the morphological data.

Drosophila Alms1 proteins regulate centriolar cartwheel assembly by enabling Plk4-Ana2 amplification loop.

Centrioles play a central role in cell division by recruiting pericentriolar material (PCM) to form the centrosome. Alterations in centriole number or function lead to various diseases including cancer or microcephaly. Centriole duplication is a highly conserved mechanism in eukaryotes.

Value of non-invasive test dynamics in guiding HCC surveillance decisions after HCV cure in patients with cirrhosis.

Background And Aims: The objective was to describe the dynamics of noninvasive tests (NITs) in cirrhotic patients following sustained virological response (SVR) and to assess their correlation with hepatocellular carcinoma (HCC) risk.

Methods: The dynamics of NITs (Fib4, APRI and LSM) were described in patients with cirrhosis after SVR included between 2006 and 2015 in two prospective French multicentre cohorts (ANRS CO22 Hepather and CO12 CirVir). To assess their relationship with the risk of HCC, a joint modeling approach was employed using both standard and flexible models adjusted for age and sex.