SMAP3-ID for Identification of Endogenous Protein-Protein Interactions Reveals Regulation of Mitochondrial Activity by Lamins.

Proteins regulate biological functions through the formation of distinct protein complexes. Identification and characterization of these protein-protein interactions are critical to deciphering their mechanism of action. Different antibody-based or cross-linking-based methods have been developed to identify the protein-protein interactions.

Photoluminescence of a Uranium(IV) Alkoxide Complex.

In this report, we describe the photoluminescence of a homoleptic uranium(IV) alkoxide complex. Excitation of [Li(THF)][U(O Bu)] leads to the first example of photoluminescence from a well-defined actinide complex originating from an f-f excitation, supported by second order multiconfigurational electronic structure calculations including spin-orbit coupling. These calculations show strong spin-orbit coupling between the excited triplet and singlet states for the 5f-orbital manifold, which leads to a long-lived excited state lifetime of 0.

Abraham Patchornik: The Contemporary Relevance of His Work for Chemistry and Biology.

Abraham Patchornik was born in 1926 in Ness Ziona, a town in Palestine founded by his great-grandfather Reuben Lehrer in 1883. He started to study chemistry as an undergraduate at the Hebrew University. However, this was interrupted by the war, and he completed his studies in various locations in West Jerusalem.

Single-Virus Microscopy of Biochemical Events in Viral Entry.

Cell entry by enveloped viruses involves a set of multistep, multivalent interactions between viral and host proteins as well as manipulation of nanoscale membrane mechanics by these interacting partners. A mechanistic understanding of these events has been challenging due to the complex nature of the interactions and the event-to-event heterogeneity involved. Single-virus microscopy has emerged as a powerful technique to probe viral binding and fusion kinetics.

Evaluating the Role of Metastable Surfaces in Mechanochemical Reduction of Molybdenum Oxide.

Mechanochemistry and mechanocatalysis are gaining increasing attention as environmentally friendly chemical processes because of their solvent-free nature and scalability. Significant effort has been devoted for studying continuum-scale phenomena in mechanochemistry, such as temperature and pressure gradients, but the atomic-scale mechanisms remain relatively unexplored. In this work, we focus on the mechanochemical reduction of MoO as a case study.

: A Model Eukaryotic Organism for Astrobiological Studies on Microbial Interactions with Martian Soil Analogs.

The exploration of our solar system for microbial extraterrestrial life is the primary goal of several space agencies. Mars has attracted substantial attention owing to its Earth-like geological history and potential niches for microbial life. This study evaluated the suitability of the polyextremophilic fungal strain LaBioMMi 1217 as a model eukaryote for astrobiology.

Anomalously High Dielectric Strength and Capacitive Energy Density of Thin Entangled Glassy Polymer Films.

The influence of high-intensity electric fields on the stability of polymeric materials is a problem of interest in the design of next-generation energy storage and electronic devices, and for understanding the limits of stability of polymer films exposed to large electric fields generally. Here, we show that the dielectric strength of entangled glassy polymer films increases as an inverse power-law of the film thickness for "ultrathin" films below a micron in thickness. The dielectric strength enhancement in these polymer films becomes as large as ≈2 GV/m in films thinner than 100 nm, but in this thickness regime, the increase of the dielectric strength depends strongly on the polymer mass, sample aging time, and the method of film preparation.

Activity-Based Bioluminescent Logic-Gate Probe Reveals Crosstalk Between the Inflammatory Tumor Microenvironment and ALDH1A1 in Cancer Cells.

Cancer cells with high expression of aldehyde dehydrogenase 1A1 (ALDH1A1) are more resistant to chemotherapy, contribute to tumor progression, and are associated with poor clinical outcomes. ALDH1A1 plays a critical role in protecting cells from reactive aldehydes and, in the case of stem cells, regulates their differentiation through the retinoic acid signaling pathway. Despite the importance of this enzyme, methods to study ALDH1A1 high-expressing cancer cells in vivo remain limited.

AshPhos Ligand: Facilitating Challenging Aminations in Five- and Six-Membered Heteroaryl Halides Using Cyclic Secondary and Bulky Amines.

Our newly developed AshPhos ligand represents a significant advancement in Buchwald-Hartwig aminations, overcoming many limitations of existing ligands. Created from affordable and accessible materials, AshPhos enhances catalytic performance, especially for extremely difficult substrates, by emphasizing the principles of ligand chelation and cooperativity. Its successful synthesis and application in catalytic aminations underscore its potential for use in the sustainable synthesis of compounds important to medicinal chemistry, materials, and energy.

Molecular Basis for Short-Chain Thioester Hydrolysis by Acyl Hydrolases in -Acyltransferase Polyketide Synthases.

Polyketide synthases (PKSs) are multidomain enzymatic assembly lines that biosynthesize a wide selection of bioactive natural products from simple building blocks. In contrast to their -acyltransferase (AT) counterparts, -AT PKSs rely on stand-alone ATs to load extender units onto acyl carrier protein (ACP) domains embedded in the core PKS machinery. -AT PKS gene clusters also encode stand-alone acyl hydrolases (AHs), which are predicted to share the overall fold of ATs but function like type II thioesterases (TEs), hydrolyzing aberrant acyl chains from ACP domains to promote biosynthetic efficiency.

My Bad, You Got This: witnessing, therapist attitude and the synergy between psychedelics and inner healing intelligence in the treatment of trauma.

The MAPS (Multidisciplinary Association for Psychedelic Studies) sponsored MDMA-assisted therapy protocol has had greater success in treating trauma in preliminary clinical trials than any prior psychotherapeutic, pharmacologic, or combined approach. It is predicated on a synergy between drug action and the participant's inner healing intelligence. The latter is described mainly by analogy with the body's capacity to heal itself, and the treatment is characterized as a means of activating or accessing this capacity.

Electrochemical Cycling of Liquid Organic Hydrogen Carriers as a Sustainable Approach for Hydrogen Storage and Transportation.

Hydrogen (H), as a high-energy-density molecule, offers a clean solution to carry energy. However, the high diffusivity and low volumetric density of H pose a challenge for long-term storage and transportation. Liquid organic hydrogen carriers (LOHCs) have been suggested as a strategic way to store and transport hydrogen in stable molecules.

Reinforcing Bulk Heterojunction Morphology through Side Chain-Engineered Pyrrolopyrrole-1,3-dione Polymeric Donors for Nonfullerene Organic Solar Cells.

Organic solar cells (OSCs) are attracting significant attention due to their low cost, lightweight, and flexible nature. The introduction of nonfullerene acceptors (NFAs) has propelled OSC development into a transformative era. However, the limited availability of wide band gap polymer donors for NFAs poses a critical challenge, hindering further advancements.

Chloride Treatments Improve Zinc Telluride Absorbers for Photoelectrochemical Carbon Dioxide Reduction.

Utilizing sunlight for photoelectrochemical carbon dioxide reduction reaction (PEC CO RR) is a carbon-neutral path to valuable liquid fuels. Higher quality photoabsorbers are needed to improve the efficiency of the PEC CO RR process. We show how the optoelectronic properties of sputtered ZnTe absorbers can be improved for this purpose via chloride treatments.

Evaluation of the Catalytic Effect of Metal Additives on the Performance of a Combined Battery and Electrolyzer System.

A low-cost method of green hydrogen production via the modification of a lead acid battery has been achieved, resulting in a hydrogen flow rate of 5.3 L min from a 20-cell string. The electrochemical behavior and catalytic effect of various metal additives on the hydrogen evolution reaction (HER) was evaluated using cyclic voltammetry.

Investigating the Effects of Copper Impurity Deposition on the Structure and Electrochemical Behavior of Hydrogen Evolution Electrocatalyst Materials.

Electrolysis of impure water (such as seawater) has recently garnered research interest as it may enable hydrogen production at reduced costs. However, the tendency of impurity ions and other species to degrade electrocatalysts and membranes within an electrolyzer is a serious challenge. Here, we investigate the effects of copper impurities of varying concentrations on the hydrogen evolution reaction (HER) using platinum electrocatalysts.

Diagnostic approach to limbal stem cell deficiency.

Limbal stem cell deficiency (LSCD) is an important cause of visual and ocular morbidity. Effective diagnosis and management require a thoughtful and comprehensive evaluation of the ocular surface. This review describes the pathogenesis, diagnosis, and grading of LSCD, as well as characteristic findings via slit lamp examination, confocal microscopy, anterior segment optical coherence tomography (AS-OCT), impression cytology, and OCT angiography.

Hantavirus antibody seroprevalence and risk factors among adults in West Kazakhstan, 2023.

Background: Orthohantaviruses (also known as hantaviruses) are pathogens, primarily transmitted by rodents, that can cause hemorrhagic fever with renal syndrome (HFRS). In endemic regions of Kazakhstan, no confirmed HFRS cases were detected between 2020 and 2022 raising concerns about detection. Estimate antibody seroprevalence for hantaviruses and identify associated risk factors among high-risk adults in western Kazakhstan in 2023.

The adaptation process of a safety planning intervention to promote connectedness and reduce distress in Diné adolescents and adults during the COVID-19 pandemic.

Introduction: Indigenous connectedness is an impetus for health, well-being, self-confidence, cultural preservation, and communal thriving. When this connectedness is disrupted, the beliefs, values, and ways of life that weave Indigenous communities together is threatened. In the Spring of 2020, the COVID-19 virus crept into Tribal Nations across the United States and exacerbated significant health-related and educational inequities.

Stepwise Chemical Reduction of [4]Cyclo[4]helicenylene: Stereo Transformation and Site-Selective Metal Complexation.

A highly strained macrocycle comprising four [4]helicene panels, [4]cyclo[4]helicenylene ([4]CH, ), was synthesized through a one-pot macrocyclization and chemically reduced by alkali metals (Na and K), revealing a four-electron reduction process. The resulting di-, tri-, and tetraanions of compound were isolated and crystallographically characterized by X-ray diffraction. Owing to the four axially chiral bi[4]helicenyl fragments, a reversible stereo transformation of between the (,,,)- and (,,,)-configurations was disclosed upon the two-electron uptake, which was rationally understood by theoretical calculations.

Social support from different sources and its relationship with stress in spaceflight analog environments.

While there is a large body of research on social support in traditional work settings, less is known about how the unique context of long-term isolation and confinement affects perceived social support. The purpose of our research was to examine how perceptions of social support change over time, how they differ by source (i.e.

"The Dollar Store Got It Going On": Understanding Food Shopping Patterns and Policy Preferences among Dollar Store Shoppers with Low Incomes.

Background: The growing dollar store sector has raised concerns about nutrition and associated health outcomes, especially for low-income communities who disproportionately rely on dollar stores. Perspectives of dollar store shoppers are largely absent.

Objective: This study aimed to understand why low-income shoppers choose to purchase food from dollar stores and what store changes, policies, and programs would make it easier for them to purchase healthier items.

The Effect of Daily Avocado Intake on Food and Nutrient Displacement in a Free-Living Population with Abdominal Obesity.

Background: Avocado intake has been associated with improvements in diet quality. Whether this response is because of avocado intake, , or combined with a food and/or nutrient displacement (D) has yet to be determined.

Objectives: This secondary analysis, conducted using dietary data from the Habitual Diet and Avocado Trial, sought to assess the effect of consuming a large avocado (168 g, 281 kcal) daily in the avocado-supplemented diet (AD) group compared with the habitual diet (HD) group on food and nutrient D.