A simple phylogenetic approach to analyze hypermutated HIV proviruses reveals insights into their dynamics and persistence during antiretroviral therapy.

Hypermutated proviruses, which arise in a single Human Immunodeficiency Virus (HIV) replication cycle when host antiviral APOBEC3 proteins introduce extensive guanine to adenine mutations throughout the viral genome, persist in all people living with HIV receiving antiretroviral therapy (ART). However, hypermutated sequences are routinely excluded from phylogenetic trees because their extensive mutations complicate phylogenetic inference, and as a result, we know relatively little about their within-host evolutionary origins and dynamics. Using >1400 longitudinal single-genome-amplified HIV sequences isolated from six women over a median of 18 years of follow-up-including plasma HIV RNA sequences collected over a median of 9 years between seroconversion and ART initiation, and >500 proviruses isolated over a median of 9 years on ART-we evaluated three approaches for masking hypermutation in nucleotide alignments.

Demographics, Epidemiology, Mortality, and Difficult-To-Treat Resistance Patterns of Bacterial Bloodstream Infections in the Global United States Military Health System from 2010-2019: A Retrospective Cohort Study.

Objective: To describe demographics, causative pathogens, hospitalization, mortality, and antimicrobial resistance of bacterial bloodstream infections (BSIs) among beneficiaries in the global U.S. Military Health System (MHS), a single-provider healthcare system with 10-year longitudinal follow-up.

A composite socioeconomic deprivation index from survey data: associations with health outcomes and disparities.

Socioeconomic deprivation - defined as a lack of social, economic and material resources - is associated with poor health outcomes and health disparities between population groups. The Research Program is a longitudinal cohort study of diverse participants from the United States, with demographic and social determinants of health data gleaned from participant surveys and health outcome data derived from electronic health records. We developed a composite index of socioeconomic deprivation (iSDI) using a cohort of 202,919 participants - based on education, employment, health insurance, housing, and income data - and we associated iSDI with health outcomes and disparities.

Plagiarism in Publications: All about Being Fair!

Upholding the highest standards of publication ethics is critical for ensuring the integrity of scholarly work, maintaining public trust, and advancing knowledge responsibly in academia. Plagiarism, defined as intellectual theft, is a significant ethical issue that undermines these principles. There are many forms of plagiarism, including direct, self, mosaic, and accidental plagiarism.

Infant action understanding: the roles of active training and motor development.

Introduction: This study examined the potential interplay between motor development and intervention in support of action understanding.

Methods: Eighty nine-month-old infants completed a tool-use training session and goal imitation paradigm that assessed action understanding in counterbalanced order. A metric of motor development was obtained using the Early Motor Questionnaire.

American society for preventive cardiology 2024 cardiovascular disease prevention: Highlights and key sessions.

Groundbreaking strategies for preventive cardiology were showcased at the 2024 American Society for Preventive Cardiology (ASPC) Congress on Cardiovascular Disease (CVD) Prevention held in Salt Lake City, Utah, from August 2nd to 4th, 2024. The event featured 69 moderators and 13 scientific sessions comprised of 98 topics, 36 satellite events, 133 poster presentations, and 27 lifestyle classes. The conference highlighted innovative strategies focused on integrating cardiovascular, kidney, and metabolic health, presenting a cohesive approach for managing complex, interrelated conditions.

Uncertainty estimation in diagnosis generation from large language models: next-word probability is not pre-test probability.

Objective: To evaluate large language models (LLMs) for pre-test diagnostic probability estimation and compare their uncertainty estimation performance with a traditional machine learning classifier.

Materials And Methods: We assessed 2 instruction-tuned LLMs, Mistral-7B-Instruct and Llama3-70B-chat-hf, on predicting binary outcomes for Sepsis, Arrhythmia, and Congestive Heart Failure (CHF) using electronic health record (EHR) data from 660 patients. Three uncertainty estimation methods-Verbalized Confidence, Token Logits, and LLM Embedding+XGB-were compared against an eXtreme Gradient Boosting (XGB) classifier trained on raw EHR data.

Current trends and future directions in probiotics research for HIV/AIDS.

Aim: This study aims to comprehensively and systematically review the current status of research on probiotics and HIV/AIDS, while also exploring future research hotspots and trends in this domain.

Methods: The Web of Science (WoS) Core Collection database was queried up until May 13, 2024, to retrieve relevant literature on probiotics and HIV/AIDS. Utilizing CiteSpace, VOSviewers, and Bibliometrix software, scientific achievements and research frontiers in this field were analyzed.

Avian influenza overview September-December 2024.

Between 21 September and 6 December 2024, 657 highly pathogenic avian influenza (HPAI) A(H5N1) and A(H5N5) virus detections were reported in domestic (341) and wild (316) birds across 27 countries in Europe. Many HPAI outbreaks in domestic birds were clustered in areas with high poultry density and characterised by secondary farm-to-farm spread. Waterfowl, particularly the mute swan, were primarily affected during this reporting period, with HPAI virus detections focused on south-eastern Europe.

Predictors of self-care in kidney transplant patients according to preoperative dialysis: A comparative study.

Background: Preemptive kidney transplantation (PKT) is associated with reduced dialysis-related morbidity and improved graft survival, which has led to an increase in the prevalence of PKT. The distinct clinical pathways of PKT and non-preemptive kidney transplantation (NPKT) patients may affect their ability to perform self-care, a key factor in post-transplant recovery. However, there is ongoing controversy regarding which group demonstrates better self-care abilities, highlighting the need to explore the factors influencing self-care in each group.

A scoping review of the Trauma Recovery Center model for underserved victims of violent crime.

Victimization in the United States is common and has long lasting negative impacts for individuals, often disproportionately impacting those of color and from low socioeconomic communities. The Trauma Recovery Center (TRC) model aims to provide comprehensive mental health and wrap-around case management services for underserved victims of crime. Following PRISMA-ScR guidelines, we sought to further our knowledge about the impact of the TRC model.

Sharing Genomic Tumor Sequencing Results With Patients: Experiences of Advanced Practice Oncology Providers.

Background: Patients with cancer routinely undergo genomic tumor sequencing, a component of molecular profiling (MP), to better characterize their cancer and identify potential targetable alterations. Targeted treatments potentially confer higher response rates and better efficacy. With increasing complexity, patients may require detailed explanations of MP results.

The Minimum Dietary Diversity For Women Indicator Can Be Extended To Children And Adolescents aged 4-15 Years As A Proxy Population Indicator For Good Micronutrient Adequacy Of Diets In Low- and Middle-Income Countries.

Background: The response to the global call for more data on children's and adolescents' diets and nutrition is limited by the lack of straightforward practical indicators to track their diet quality. On the basis of a food group score compiled from 10 food groups (FGS-10), the minimum dietary diversity for women, calculated as FGS-10 ≥ 5, is a validated proxy population indicator for better micronutrient intake adequacy for adult women in low- and middle-income countries (LMICs).

Objectives: This study aims to validate FGS-10 and its related cutoffs against micronutrient intake adequacy in 4-15-y-old children/adolescents in LMICs.

Pooled microarray expression analysis of failing left ventricles reveals extensive cellular-level dysregulation independent of age and sex.

Existing cardiovascular studies tend to suffer from small sample sizes and unaddressed confounders. Re-profiling of 9 microarray datasets revealed significant global gene expression differences between 358 failing and 191 non-failing left ventricles independent of age and sex ( = 5.1e-10).

Socioeconomic/health-related factors associated with HPV vaccination initiation/completion among females of paediatric age: A systematic review with meta-analysis.

Objectives: To systematically identify, appraise, and summarise published evidence on individual socioeconomic and health-related factors associated with human papillomavirus (HPV) vaccination initiation and completion among females of paediatric age.

Study Design: A global systematic review with meta-analysis (PROSPERO: CRD42023445721).

Methods: We performed a literature search in December 2022 and supplemented the search on August 1, 2023.

A standards-based application for improving platelet transfusion workflow.

Objective: Thrombocytopenia is a common complication of hematopoietic stem-cell transplantation (HSCT), though many patients will become immune refractory to platelet transfusions over time. We built and evaluated an electronic health record (EHR)-integrated, standards-based application that enables blood-bank clinicians to match platelet inventory with patients using data previously not available at the point-of-care, like human leukocyte antigen (HLA) data for donors and recipients.

Materials And Methods: The web-based application launches as an EHR-embedded application or as a standalone application.

Hidden in plain sight: abdominopelvic pain unveiling a Spigelian hernia containing ovary and fallopian tube.

Spigelian hernias are rare clinical entities; vague symptomatology and unreliable clinical examination ensure difficult diagnosis. Computed tomography (CT) is critical for accurate diagnosis. Surgical repair is mandated given the high risk of visceral organ incarceration.

Improving Access to Early Developmental Evaluation in Academic Primary Care Centers.

Introduction: Developmental disorders (DDs) affect approximately 1 in 6 children in the United States. Early identification and treatment improve developmental outcomes and child and family functioning. Disparities exist in the diagnosis of DD that leads to inequitable access to developmental services during important periods of neuroplasticity.

Instagram Versus Reality: Who Are Actually Plastic Surgeons?

Background: Instagram has become one of the most powerful marketing tools available to plastic surgeons because patients have increasingly turned to online resources to find physicians. Within, we review the online presence of self-ascribed plastic surgeons in the United States to identify potential misinformation and dishonest advertising.

Methods: The Inflact database was queried for the search terms: "plastic surgeon/surgery," "plastic and reconstructive surgeon/surgery," "aesthetic surgeon/surgery," and "cosmetic surgeon/surgery.

Ethnically Diverse Adolescents Recount Third-Party Actions That Amplify Their Anger and Calm Their Emotions After Perceived Victimization.

Third-party adolescents (those aware of peer conflict as neither aggressors nor victims) can help shape their peers' emotional responses to perceived victimization. Emotion regulation promotes resilience for those who have been victimized, and heightened anger can exacerbate negative outcomes. This study sought to understand how ethnically diverse victimized adolescents described third-party actions that amplified their anger and calmed their emotions-and whether there were gender, racial/ethnic, or school-level patterns.

Diabetes medication prescribing trends based on provider type and location in the United States.

Background: As primary care physician numbers continue to decline, more patients with type 2 diabetes are likely to receive care from advanced practice providers (APPs), including physician assistants and nurse practitioners. Analyzing diabetes medication prescribing trends among these provider types is essential for ensuring evidence-based diabetes care. This retrospective, cross-sectional pilot study aimed to examine differences in type 2 diabetes medication prescribing trends by provider type (physicians vs.

Temporal and spatial pattern analysis of escaped prescribed fires in California from 1991 to 2020.

Background: Prescribed fires play a critical role in reducing the intensity and severity of future wildfires by systematically and widely consuming accumulated vegetation fuel. While the current probability of prescribed fire escape in the United States stands very low, their consequential impact, particularly the large wildfires they cause, raises substantial concerns. The most direct way of understanding this trade-off between wildfire risk reduction and prescribed fire escapes is to explore patterns in the historical prescribed fire records.

A morpho-histological analysis of the exoskeleton of (Cnidaria, Hydrozoa) reveals insights into the taxonomy of Clathrozoellidae and Hydroidolina.

The taxonomic complexity of the families Clathrozoidae and Clathrozoellidae, rooted in early 20th-century hydroid descriptions, highlights the need for comprehensive and detailed morphological analyses. This study aimed to elucidate the histology of the polypoid stage of Peña Cantero, Vervoort & Watson, 2003, with a particular emphasis on its exoskeletal structure. Specimens from the National Museum of Natural History were examined histologically using different staining techniques.

Reproductive strategies in loggerhead sea turtle : polyandry and polygyny in a Southwest Atlantic rookery.

Sea turtles are highly migratory and predominantly inhabit oceanic environments, which poses significant challenges to the study of their life cycles. Research has traditionally focused on nesting females, utilizing nest counts and mark-recapture methods, while male behavior remains understudied. To address this gap, previous studies have analyzed the genotypes of females and hatchlings to indirectly infer male genotypes and evaluate the extent of multiple paternity within populations.