Deep learning-based design and experimental validation of a medicine-like human antibody library.

Antibody generation requires the use of one or more time-consuming methods, namely animal immunization, and in vitro display technologies. However, the recent availability of large amounts of antibody sequence and structural data in the public domain along with the advent of generative deep learning algorithms raises the possibility of computationally generating novel antibody sequences with desirable developability attributes. Here, we describe a deep learning model for computationally generating libraries of highly human antibody variable regions whose intrinsic physicochemical properties resemble those of the variable regions of the marketed antibody-based biotherapeutics (medicine-likeness).

High-Performance TiCT-MXene/Mycelium Hybrid Membrane for Efficient Lead Remediation: Design and Mechanistic Insights.

This study presents a hybrid microfiltration technology designed for high-performance lead (Pb(II)) remediation, especially from aqueous solutions with high Pb(II) concentrations, by utilizing two-dimensional (2D) TiCT-MXene layers deposited on dry mycelium membranes. The hybrid TiCT-MXene/mycelium (MyMX) membranes were fabricated via a single-step electrochemical deposition (ECD) technique, which enabled a uniform coating of 2D TiCT-MXene onto individual hyphal fibers of a prefabricated mycelium membrane. Optimized ECD parameters for high Pb(II) uptake were identified using scanning electron microscopy and energy-dispersive X-ray spectroscopy.

Economic Evaluations of Music Therapy and Other Music-Based Interventions: A Scoping Review.

The cost-effectiveness of an intervention is an important factor in health care decisions about which health care services should be publicly funded and/or approved as an eligible intervention for private insurance coverage. Music therapy as a health profession lacks substantial research on the cost-effectiveness of its services and there is no overview of existing data. We therefore conducted a scoping review.

Intravascular Imaging as a Performance Measure for Percutaneous Coronary Intervention.

Background: Intravascular imaging (IVI) is widely recognized to improve outcomes after percutaneous coronary intervention (PCI). However, IVI is underutilized and is not yet established as a performance measure for quality PCI.

Methods: We examined temporal trends of IVI use for all PCIs performed at Veterans Affairs hospitals in the United States from 2010 to 2022 using retrospective observational cohorts.

Occupational therapists' experiences of hegemony in a mental health setting: A practice-based enquiry.

Background: Despite valuing occupation, occupational therapists report barriers to enacting occupation-based practice. One barrier noted in the literature is hegemony, the dominance of one social group's ideas over others. Specifically, biomedical and business models dominating healthcare are reported to significantly impact occupational therapists.

External validation of the SORG machine learning for 90-day and 1-year mortality in patients suffering from extremity metastatic disease in an European cohort of 174 patients.

Accurate survival prediction of patients with long-bone metastases is challenging, but important for optimizing treatment. The Skeletal Oncology Research Group (SORG) machine learning algorithm (MLA) has been previously developed and internally validated to predict 90-day and 1-year survival. External validation showed promise in the United States and Taiwan.

The top 100 most-cited total knee arthroplasty publications.

The object is to objectively identify the 100 most influential scientific publications in total knee arthroplasty (TKA) and provide an analysis of their main characteristics. The Clarivate Analytics Web of Knowledge database was used to obtain data and metrics of TKA research. The search list was sorted by the number of citations, and articles were included or excluded based on relevance to TKA.

Comparative Genomic Analysis Reveals the Emergence and Dissemination of Different serovar Gallinarum biovar Gallinarum Lineages in Brazil.

serovar Gallinarum biovar Gallinarum is a pathogenic bacterium that causes fowl typhoid (FT), affecting chicken flocks worldwide. This study aimed to evaluate the emergence, dissemination and genomic profile of Gallinarum lineages from Brazil. Twelve whole-genomes sequences (WGS) of different .

Glial activation and nociceptive neuropeptide elevation associated with the development of chronic post-traumatic headache following repetitive blast exposure.

Chronic headaches and pain are prevalent in those who are exposure to blast events, yet there is a gap in fundamental data that identifies the pathological mechanism for the chronification of pain. Blast-related post-traumatic headaches (PTH) are understudied and chronic pain behaviors in preclinical models can be vital to help elucidate PTH mechanisms. The descending pain modulatory system controls pain perception and involves specific brain regions such as the cortex, thalamus, pons, and medulla.

Effects of the narrative elaboration technique and open-ended rapport on accuracy of children's recall according to age.

Introduction: This study explored the impacts of the narrative elaboration technique (NET) and open-ended rapport building on younger ( = 30, ages 5-6) and older ( = 30, ages 7-8) children's free recall and suggestibility.

Methods: Children were randomly assigned to either a NET condition or an open-ended rapport condition after engaging in a photo-taking play session with an experimenter. Then, a novel interviewer asked them about the play session.

Floral traits underlying mating system differentiation in the wind-pollinated sister species and .

The shift from outcrossing to predominantly selfing is one of the most common transitions in plant evolution. This evolutionary shift has received considerable attention from biologists; however, this work has almost exclusively been focused on animal-pollinated systems. Despite the seminal ecological and economic importance of wind-pollinated species, the mechanisms controlling the degree of outcrossing in wind-pollinated taxa remain poorly understood.

Growth-friendly spinal surgery: Review of the effect on truncal growth.

Background: Pediatric spinal deformity surgery affects ultimate spinal height in the growing child. This effect on ultimate spinal height has also been shown to affect pulmonary development and ultimately pulmonary function. There has been an increasing trend toward growth-friendly spinal surgery in early onset scoliosis to minimize the negative consequences of early spinal fusion surgery.

1990-2041 Global burden of Burkitt lymphoma with 20 years forecast: A systematic analysis using the global burden disease of study of 2021.

Background: To investigate the trends in Burkitt lymphoma incidence, mortality, and disability-adjusted life-years, considering sex and age, from 1990 to 2021, with a 20-year forecast.

Method: Data regarding Burkitt lymphoma were extracted from the Global Burden of Disease study for the year 2021.

Results: Globally, there were 19,072 incident cases of Burkitt lymphoma in 2021.

Temporal Trends of Fungal Infections in Cirrhotic Patients: A Retrospective Cohort Study 2016-2020.

Background: Patients with cirrhosis are susceptible to infections due to abnormalities in humoral and cell-mediated immunity. Fungal infections are associated with delayed diagnosis and high mortality rates, emphasizing the importance of performing fungal cultures and maintaining elevated levels of suspicion in this patient population.

Methods: This retrospective cohort study analyzes cirrhotic patients readmitted with bacterial and fungal infections and investigates outcomes, including in-hospital mortality and hospital resource utilization.

Breaking Barriers: Addressing Gender Disparities in Hip Resurfacing Surgery Access in the United States.

Second-generation large-diameter head hip resurfacing (HR) arthroplasty has gained popularity in terms of its potential for minimal wear and the preservation of proximal femoral bone stock. HR faces challenges, such as increased hip fracture rates and adverse reactions to metal detritus, despite the fact that over one million metal-on-metal (MoM) arthroplasties have been performed globally. FDA issued warnings in 2011 and 2016 regarding higher failure rates in women and categorized MoM implants as high-risk, influencing U.

Insights into anesthesia administration for elderly individuals undergoing painless gastroenteroscopy: A bibliometric study.

Background: Administering anesthesia to elderly patients undergoing gastroenteroscopy necessitates careful attention due to age-related physiological changes and an increased risk of complications.

Aim: To analyze the research trends in anesthesia management for elderly patients undergoing gastroenteroscopy.

Methods: We performed a literature search using the Web of Science database to identify articles published between 2004 and 2023.

Squamate reptiles may have compensated for the lack of γδTCR with a duplication of the TRB locus.

Squamate reptiles are amongst the most successful terrestrial vertebrate lineages, with over 10,000 species across a broad range of ecosystems. Despite their success, squamates are also amongst the least studied lineages immunologically. Recently, a universal lack of γδ T cells in squamates due to deletions of the genes encoding the T cell receptor (TCR) γ and δ chains was discovered.

Injecting hope: the potential of intratumoral immunotherapy for locally advanced and metastatic cancer.

Despite enormous progress, advanced cancers are still one of the most serious medical problems in current society. Although various agents and therapeutic strategies with anticancer activity are known and used, they often fail to achieve satisfactory long-term patient outcomes and survival. Recently, immunotherapy has shown success in patients by harnessing important interactions between the immune system and cancer.

promotes synchronous multiple primary lung cancer progression through apoptosis regulation.

Background: Dysbiosis of the lung microbiome can contribute to the initiation and progression of lung cancer. Synchronous multiple primary lung cancer (sMPLC) is an increasingly recognized subtype of lung cancer characterized by high morbidity, difficulties in early detection, poor prognosis, and substantial clinical challenges. However, the relationship between sMPLC pathogenesis and changes in the lung microbiome remains unclear.

ESAT-6 protein suppresses allograft rejection by inducing CD4Foxp3 regulatory T cells through IκBα/cRel pathway.

Background: Maintenance immunosuppression is required for suppression of alloimmunity or allograft rejection. However, continuous use of immunosuppressants may lead to various side effects, necessitating the use of alternative immunosuppressive drugs. The early secreted antigenic target of 6 kDa (ESAT-6) is a virulence factor and immunoregulatory protein of mycobacterium tuberculosis (Mtb), which alters host immunity through dually regulating development or activation of various immune cells.

Synergetic role of TRPV4 inhibitor and mechanical loading on reducing inflammation.

Resolution of inflammation is essential for normal tissue healing and regeneration, with macrophages playing a key role in regulating this process through phenotypic changes from a pro-inflammatory to an anti-inflammatory state. Pharmacological and mechanical (mechanotherapy) techniques can be employed to polarize macrophages toward an anti-inflammatory phenotype, thereby diminishing inflammation. One clinically relevant pharmacological approach is the inhibition of Transient Receptor Potential Vanilloid 4 (TRPV4).

Recognition of by the pattern recognition molecules langerin, mannan-binding lectin, and surfactant protein D: the influence of capsular polysaccharides and wall teichoic acid.

The innate immune system plays a critical role in the rapid recognition and elimination of pathogens through pattern recognition receptors (PRRs). Among these PRRs are the C-type lectins (CTLs) langerin, mannan-binding lectin (MBL), and surfactant protein D (SP-D), which recognize carbohydrate patterns on pathogens. Each represents proteins from different compartments of the body and employs separate effector mechanisms.

Advancing precision cancer immunotherapy drug development, administration, and response prediction with AI-enabled Raman spectroscopy.

Molecular characterization of tumors is essential to identify predictive biomarkers that inform treatment decisions and improve precision immunotherapy development and administration. However, challenges such as the heterogeneity of tumors and patient responses, limited efficacy of current biomarkers, and the predominant reliance on single-omics data, have hindered advances in accurately predicting treatment outcomes. Standard therapy generally applies a "one size fits all" approach, which not only provides ineffective or limited responses, but also an increased risk of off-target toxicities and acceleration of resistance mechanisms or adverse effects.

A comparative study of hospitalization costs of TKA inpatients before and after National Volume-based Procurement in Guangdong, China: an interrupted time-series analysis.

Background: This study aims to explore the impact of the National Volume-based Procurement Policy in Guangdong Province on hospitalization costs for total knee arthroplasty inpatients.

Methods: Interrupted time-series analysis were used to examine the expenses associated with total knee arthroplasty for inpatients at a hospital in Guangzhou between May 10, 2021, and December 26, 2023. The period was divided into two phases based on the implementation of the policy, the pre-policy phase (May 10, 2021, to April 30, 2022) and the post-policy phase (May 1, 2022, to December 26, 2023).