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In vitro culture of previously uncultured oral bacterial phylotypes.
Appl Environ Microbiol
December 2015
Centre for Immunology and Infectious Disease, Barts and The London School of Medicine and Dentistry, Queen Mary University of London, London, United Kingdom Forsyth Institute, Cambridge, Massachusetts, USA
Around a third of oral bacteria cannot be grown using conventional bacteriological culture media. Community profiling targeting 16S rRNA and shotgun metagenomics methods have proved valuable in revealing the complexity of the oral bacterial community. Studies investigating the role of oral bacteria in health and disease require phenotypic characterizations that are possible only with live cultures.
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