[Analysis on relative factors of influencing limb functional recovery in the patient of hemiplegia].

Objective: To investigate relative factors of influencing limb functional recovery in the patient of hemiplegia.

Methods: In 312 stroke patients with hemiplegia who were treated by a comprehensive rehabilitation program, 10 factors of possibly influencing limb functional recovery in the patient of hemiplegia were selected to make retrospective analysis and study on 48 cases with no improvement of limb function after treatment.

Results: Functional recovery of hemiplegic limbs was not correlated with gender and location of brain lesion, but was correlated with the age and courses of disease, types of stroke, aphasis, psychic disturbance as well as complications (P < 0.

[A new thinking of acupuncture and moxibustion treatment of shoulder pain after hemiplegia].

Analysis from anatomy and physiology indicates that causes of shoulder pain are (1) decline and after-constriction of the scapula;(2) the adduction and intorsion of the humerus; (3) the spasm of the upper limb flexor. The acupuncture and moxibustion prescriptions stipulated for prevention and treatment of shoulder pain are Jianjing (GB 21), Jianzhongshu (SI 15), Jugu (LI 16), Tianzong (SI 11), Jianliao (SJ 14), Jiquan (HT 1) at the flaccid hemiplegia, and Jugu (LI 16), Jianyu (LI 15), Tianzong (SI 11), Jianliao (SJ 14), Xiaoluo (SJ 12), Shanglian (LI 9), Wenliu (LI 7) at the spastic hemiplegia , in combination with cupping at the Urinary Bladder Meridian on the back and around shoulder. Thus, it is indicated that shoulder pain not only is involved in the shoulder joint but also in all muscle groups of the shoulder girdle and the upper limb extensor at the affected side.

[Construction of eukaryotic expression vector of CD80-IgG1 Fc fragment fusion protein and its expression in CHO cells].

Aim: To construct an eukaryotic expression vector of CD80-IgG1 Fc, and to express the fusion protein in CHO cells.

Methods: The gene encoding the CD80-IgG1 Fc fusion protein were constructed in eukaryotic expression vector pcDNA3.1(+) by means of T-A cloning and subcloning techniques, then was transfected into CHO cells for stable expression.

[Prognostic factors for brain metastatic tumors treated with stereotactic radiosurgery].

Objective: To assess the clinical outcome of brain metastatic tumors treated with linac-based stereotactic radiosurgery (SRS) and evaluate the potential prognostic factors.

Methods: We reviewed 102 patients with 131 brain metastatic tumors treated with a linac-based SRS from 1994 to 2002, including 78 patients with solitary and 24 with multiple brain metastases, with the diameter of tumor all within 4 cm. Among these 102 patients, 18 received planned whole-brain radiation therapy (WBRT) before or after SRS.

[Effect of antisense CD40 RNA on CD40 expression and proliferation of EB virus-transformed B lymphocytes].

Aim: To investigate the changes of CD40 expression and proliferation pattern of EB virus-transformed B lymphocytes that had been transfected by antisense CD40 RNA.

Methods: Eukaryotic expression vector pcDNA3/CD40 of human CD40antisense RNA was constructed by means of T-A cloning and subcloning, and then was transfected into EB virus-transformed B lymphocytes. Changes of CD40 expression on the B cells and their proliferation were tested by flow cytometry and MTT colorimetry assay, respectively.

Nitric oxide synthase expression in human bladder cancer and its relation to angiogenesis.

Nitric oxide (NO) is synthesized by the enzyme family of nitric oxide synthases (NOS) and plays an important role in tumor growth and angiogenesis. The expression of two of the NOS isoforms, the endothelial and inducible isoforms (eNOS and iNOS, respectively), were evaluated in bladder tissue from patients with transitional cell carcinoma (TCC). The specimens were procured from 58 patients with TCC and 14 cases of normal bladder mucosa were used as a control group.

[Bcl-2 antisense oligonucleotides suppress HL-60 cell growth in a SCID mouse mode].

Objective: To study the effects of bcl-2 antisense phosphorothioate oligonucleotides (ASPO) on suppression of HL-60 cell growth in SCID mice and to investigate the feasibility of purging leukemia cells plus bcl-2 ASPO used in vitro.

Methods: 1 x 10(7) viable HL-60 cells were treated with 10 micro mol/L bcl-2 ASPO seven days before the intraperitoneal (IP) inoculation to the SCID mice, Treatment with sense oligonucleotides (SPO) was similar as for the controls. 35 days after the inoculation, all the SCID mice of both groups were sacrificed and their peripheral blood, bone marrow, liver and spleen were examined using half nested RT-PCR and histopathology for detecting the appearance and distribution of the HL-60 cells treated beforehand with antisense or sense oligonucleotides respectively.

[Screening the hepatitis B virus PreS1 associated protein by the yeast two-hybrid system].

Objective: To screen the hepatitis B virus PreS1 associated protein from normal human liver cDNA library by the yeast two-hybrid system and explore the role of PreS1 protein in the infection of hepatitis B virus (HBV).

Methods: PCR was preformed to amplify the PreS1 gene containing EcoRI and PstI from HBV positive serum, and the production was inserted into plasmid pAS2-1 after digesting with the former two restricted endonuclease, then the bait vector pAS2-1-PreS1 was verified by auto-sequencing assay. The PreS1-BD fusion protein expressed in the yeast cells was confirmed by western blot, after pAS2-1-PreS1 was transfected into the yeast cell AH109.

[Determining blood parameters FT3, FT4, T, E2 and cortisol to explore nature of cold syndrome and heat syndrome].

Objective: To explore the relationship between the levels of free triiodothyronine (FT3), free thyroxine (FT4), cortisol (CO), testosterone (T), serum estradiol (E2) and Cold Syndrome and Heat Syndrome in traditional Chinese medicine.

Methods: The blood levels of FT3, FT4, T, E2, CO in groups of sthenic Heat (SH), sthenic Cold (SC), asthenic Heat (AH) and asthenic Cold (AC) and the healthy control group were measured. The number of cases in each group was 50.

[Effect of p53 gene on telomerase activity and hTERT gene expression in HL-60 cells].

To investigate the change of telomerase activity and human telomerase reverse transcriptase (hTERT) gene expression in HL-60 cells transfected with wild type p53 gene, wild type p53 gene was introduced into HL-60 cells by Lipofectin transfection. Apoptosis was analyzed by TUNEL assay. Telomerase activity and the level of hTERT mRNA were detected by telomeric repeat amplification protocol (TRAP)-ELISA and RT-PCR, respectively.

[The study of inhibitory and apoptosis of K562 cells by antisense oligodeoxynucleotides].

Aim: To study the effect of bcr-abl fusion gene antisense phosphorothioate oligodeoxynucleotide (Aspo) on bcr-abl mRNA and apoptosis of K562 cells.

Methods: Cells were exposed to Aspo. P210 was measured by Flow Cytometry.

[Study on microsatellite instability in gastric cancer].

Objective: To evaluate the microsatellite instability (MIN) in gastric cancers (GC).

Methods: MIN was examined in 106 cases of GC, using the radioactive polymerase chain reaction-based method.

Results: The frequency of MIN was significantly higher in expanding type of GC as compared with the infiltrative type (P < 0.

[Inhibition of Bcl-2 expression in HL60 cells by incubation with antisense phosphorothioate oligodeoxynucleotides].

Objective: To observe whether the expression of Bcl-2 mRNA and protein in HL60 cells could be decreased by incubation with antisense phosphorothioate oligodeoxnucleotides (ASPO) at different concentrations (5micromol/L,10micromol/L,and 20micromol/L).

Methods: The expressions of Bcl-2 protein and mRNA in HL60 cells were individually determined by immunocytochemistry, flow cytometry and RT-PCR.

Results: In the presence of ASPO, the levels of Bcl-2 mRNA and protein could be decreased.