UDP-glucose pyrophosphorylase as a target for regulating carbon flux distribution and antioxidant capacity in Phaeodactylum tricornutum.

UDP-glucose pyrophosphorylase (UGPase) is a key enzyme for polysaccharide synthesis, and its role in plants and bacteria is well established; however, its functions in unicellular microalgae remain ill-defined. Here, we perform bioinformatics, subcellular localization as well as in vitro and in vivo analyses to elucidate the functions of two UGPs (UGP1 and UGP2) in the model microalga Phaeodactylum tricornutum. Despite differences in amino acid sequence, substrate specificity, and subcellular localization between UGP1 and UGP2, both enzymes can efficiently increase the production of chrysolaminarin (Chrl) or lipids by regulating carbon flux distribution without impairing growth and photosynthesis in transgenic strains.

Cytoophidia and filaments: you must unlearn what you have learned.

The nucleotide CTP can be synthesized de novo from UTP via the metabolic enzyme CTP synthase (CTPS). As a textbook enzyme, CTPS has been extensively studied for seven decades. However, it came as a surprise when CTPS was found to form snake-shaped mesoscale cytoophidia in fruit fly cells.

Experimental reduction in blood oxygen-carrying capacity alters foraging behaviour in a colonial waterbird.

Oxidative metabolism is a key component of organismal physiology and it is primarily determined by aerobic capacity, which depends on the capacity of blood to carry oxygen. However, experimental manipulations of blood oxygen-carrying capacity are rarely implemented to test ecophysiological hypotheses in vertebrate populations. Here, we combined an experimental manipulation of blood oxygen-carrying capacity with GPS tracking to test whether suboptimal (reduced) haematological performance affects foraging behaviour in a colonial waterbird, the black-headed gull, Chroicocephalus ridibundus.

Epitomizing biohydrogen production from microbes: Critical challenges vs opportunities.

Hydrogen is a clean and green biofuel choice for the future because it is carbon-free, non-toxic, and has high energy conversion efficiency. In exploiting hydrogen as the main energy, guidelines for implementing the hydrogen economy and roadmaps for the developments of hydrogen technology have been released by several countries. Besides, this review also unveils various hydrogen storage methods and applications of hydrogen in transportation industry.

Impact of Negative Feedbacks on De Novo Pyrimidines Biosynthesis in .

Earlier studies aimed at investigating the metabolism of endogenous nucleoside triphosphates in synchronous cultures of cells revealed an auto-oscillatory mode of functioning of the pyrimidine and purine nucleotide biosynthesis system, which the authors associated with the dynamics of cell division. Theoretically, this system has an intrinsic oscillatory potential, since the dynamics of its functioning are controlled through feedback mechanisms. The question of whether the nucleotide biosynthesis system has its own oscillatory circuit is still open.

An assessment of the spatio-temporal dynamics of Landsat-derived aerosol concentration in relation with land cover and road networks in the Lagos megacity.

The interrelationships between air quality, land cover change, and road networks in the Lagos megacity have not been explored. Globally, there are knowledge gaps in understanding these dynamics, especially using remote sensing data. This study used multi-temporal and multi-spectral Landsat imageries at four epochs (2002, 2013, 2015, and 2020) to evaluate the aerosol optical thickness (AOT) levels in relation to land cover and road networks in the Lagos megacity.

Comparison of the Effectiveness and Environmental Impact of Selected Methods for the Determination of Fatty Acids in Milk Samples.

Determination of the fatty acid profile in milk samples is one of the most important in food analysis. There are many methodologies for FA determination. The conventional procedure for determining the FA composition of milk is isolation of fat or indirect methylation, trans-methylation, extraction of fatty acids, and analysis by gas chromatography.

Optimization of CO Sorption onto Spent Shale with Diethylenetriamine (DETA) and Ethylenediamine (EDA).

A novel technique was employed to optimize the CO sorption performance of spent shale at elevated pressure-temperature (PT) conditions. Four samples of spent shale prepared from the pyrolysis of oil shale under an anoxic condition were further modified with diethylenetriamine (DETA) and ethylenediamine (EDA) through the impregnation technique to investigate the variations in their physicochemical characteristics and sorption performance. The textural and structural properties of the DETA- and EDA- modified samples revealed a decrease in the surface area from tens of m/g to a unit of m/g due to the amine group dispersing into the available pores, but the pore sizes drastically increased to macropores and led to the creation of micropores.

Terminal Uridylyltransferases TUT4/7 Regulate microRNA and mRNA Homeostasis.

The terminal nucleotidyltransferases TUT4 and TUT7 (TUT4/7) regulate miRNA and mRNA stability by 3' end uridylation. In humans, TUT4/7 polyuridylates both mRNA and pre-miRNA, leading to degradation by the U-specific exonuclease DIS3L2. We investigate the role of uridylation-dependent decay in maintaining the transcriptome by transcriptionally profiling TUT4/7 deleted cells.

Synthesis, Characteristics, and Effect of Zinc Oxide and Silver Nanoparticles on the In Vitro Regeneration and Biochemical Profile of Chrysanthemum Adventitious Shoots.

Studies on nanoparticles' effects on plants are relevant for horticulture. This study aimed to test the influence of zinc oxide submicron particles (ZnO SMPs), zinc oxide nanoparticles (ZnO NPs), and zinc oxide nanoparticles combined with silver nanoparticles (ZnO+1%Ag NPs) applied at 100 and 500 mg·L on the regeneration and biochemical activity of adventitious shoots in (Ramat.) Hemsl.

Modeling an immune response to influenza A virus infection in alveolar epithelial cells.

Influenza A viruses (IAV) have been the cause of several influenza pandemics in history and are a significant threat for the next global pandemic. Hospitalized influenza patients often have excess interferon production and a dysregulated immune response to the IAV infection. Obtaining a better understanding of the mechanisms of IAV infection that induce these harmful effects would help drug developers and health professionals create more effective treatments for IAV infection and improve patient outcomes.

Gaussian Process for Machine Learning-Based Fatigue Life Prediction Model under Multiaxial Stress-Strain Conditions.

In this paper, a new method for fatigue life prediction under multiaxial stress-strain conditions is developed. The method applies machine learning with the Gaussian process for regression to build a fatigue model. The fatigue failure mechanisms are reflected in the model by the application of the physics-based stress and strain invariants as input quantities.

Online Managerial Accounting education during the COVID-19 pandemic: A case study from Poland.

The COVID-19 pandemic made the higher education providers give up traditional face-to-face education and change completely into distance learning. The primary objective of the study was to learn the opinions of the students majoring in Finance and Accounting at the Faculty of Management, UTP University of Science and Technology in Bydgoszcz on the Managerial Accounting (MA) course in computer laboratory taught in a form of e-learning with the use of spreadsheet. The study questions covered the effectiveness of online learning, its disadvantages and advantages, the evaluation of the skills acquired, the tutors' preparation and engagement, education quality enhancement in the second e-learning semester and suggestions in terms of the use of e-learning in post-pandemic time.

GO/TiO-Related Nanocomposites as Photocatalysts for Pollutant Removal in Wastewater Treatment.

Water pollution has been a prevalent issue globally for some time. Some pollutants are released into the water system without treatment, making the water not suitable for consumption. This problem may lead to more grave problems in the future including the destruction of the ecosystem along with the organisms inhabiting it, and illness and diseases endangering human health.

Current perspectives, future challenges and key technologies of biohydrogen production for building a carbon-neutral future: A review.

The ever-increasing quantity of greenhouse gases in the atmosphere can be attributed to the rapid increase in the world population as well as the expansion of globalization. Hence, achieving carbon neutrality by 2050 stands as a challenging task to accomplish. Global industrialization had necessitated the need to enhance the current production systems to reduce greenhouse gases emission, whilst promoting the capture of carbon dioxide from atmosphere.

Molecular mechanism underlying the di-uridylation activity of Arabidopsis TUTase URT1.

In Arabidopsis, HESO1 and URT1 act cooperatively on unmethylated miRNA and mRNA uridylation to induce their degradation. Their collaboration significantly impacts RNA metabolism in plants. However, the molecular mechanism determining the functional difference and complementarity of these two enzymes remains unclear.

Uridylation and the SKI complex orchestrate the Calvin cycle of photosynthesis through RNA surveillance of in Arabidopsis.

RNA uridylation, catalyzed by terminal uridylyl transferases (TUTases), represents a conserved and widespread posttranscriptional RNA modification in eukaryotes that affects RNA metabolism. In plants, several TUTases, including HEN1 SUPPRESSOR 1 (HESO1) and UTP: RNA URIDYLYLTRANSFERASE (URT1), have been characterized through genetic and biochemical approaches. However, little is known about their physiological significance during plant development.

Insight into microwave-assisted synthesis of the chitosan-MOF composite: Pb(II) adsorption.

Heavy metal contamination has increased over the globe, causing significant environmental issues owing to direct and indirect releases into water bodies. As a result, metal removal from water entities must be addressed soon. Various adsorbents such as MOFs and chitosan have demonstrated promising results in water treatment.

Unified DeepLabV3+ for Semi-Dark Image Semantic Segmentation.

Semantic segmentation for accurate visual perception is a critical task in computer vision. In principle, the automatic classification of dynamic visual scenes using predefined object classes remains unresolved. The challenging problems of learning deep convolution neural networks, specifically ResNet-based DeepLabV3+ (the most recent version), are threefold.

Recent Developments in Ionic Liquid-Assisted Topical and Transdermal Drug Delivery.

Ionic liquids (ILs) have attracted growing interest as designer solvents/materials for exploring unrealized functions in many areas of research including drug formulations and delivery owing to their inherent tunable physicochemical and biological properties. The use of ILs in the pharmaceutical industry can address challenges related to the use of conventional organic solvent-based chemical permeation enhancers. Their tunability in forming ion pairs with a diverse range of ions enables the task-specific optimization of ILs at the molecular level.

Determining the Role of UTP-Glucose-1-Phosphate Uridylyltransferase (GalU) in Improving the Resistance of NCFM to Freeze-Drying.

NCFM is widely used in the fermentation industry; using it as a freeze-dried powder can greatly reduce the costs associated with packaging and transportation, and even prolong the storage period. Previously published research has reported that the expression of (EC: 2.7.

Pathway Optimization and Uridine 5'-Triphosphate Regeneration for Enhancing Lacto--Tetraose Biosynthesis in Engineered .

Recently, human milk oligosaccharides (HMOs) have attracted increasing attention and display great commercial importance, especially for the infant formula industry. Lacto--tetraose (LNT) is an important neutral HMO commercially added in infant formula and a core structure for synthesizing complex HMOs. Previously, a novel LNT-generating β-1,3-galactosyltransferase from was identified and used for construction of an LNT-producing engineered .

Entropy of timekeeping in a mechanical clock.

The dynamics of a unique type of clock mechanism known as grasshopper escapement is investigated with the aim of evaluating its accuracy in a noisy environment. It is demonstrated that the clock's precision scales linearly with the rate of its entropy production, consistently with recently reported results regarding nanoscale and quantum clocks. Moreover, it is shown that the inevitable force variations present in the mechanism can be modeled with a Maxwell-Boltzmann statistic.