The intensive use of intraspecific aflatoxin biocontrol agents (BCAs) poses a risk of developing super-toxigenic strains through recombination, while alternating with interspecific BCAs is safer.
More than 50% of rice-associated biocontrol agents (RABs) showed the ability to inhibit multiple aflatoxin strains, with RAB4R being notably effective by lysing fungal hyphal tips.
Field trials demonstrated that RAB4R successfully reduced aflatoxin contamination in maize from a highly toxigenic strain, while RAB1, although less effective in growth suppression, substantially inhibited aflatoxin production and could serve as a safe companion BCA due to its lower toxicity.