13 results match your criteria: "USA. Washington National Primate Research Center[Affiliation]"
Induction of Heterologous Tier 2 HIV-1-Neutralizing and Cross-Reactive V1/V2-Specific Antibodies in Rabbits by Prime-Boost Immunization.
J Virol
October 2016
Department of Microbiology, University of Washington, Seattle, Washington, USA Washington National Primate Research Center, Seattle, Washington, USA Department of Pharmaceutics, University of Washington, Seattle, Washington, USA
Unlabelled: Poxvirus prime-protein boost used in the RV144 trial remains the only immunization strategy shown to elicit a modest level of protection against HIV-1 acquisition in humans. Although neutralizing antibodies (NAb) were generated, they were against sensitive viruses, not the more resistant "tier 2" isolates that dominate circulating strains. Instead, risk reduction correlated with antibodies recognizing epitopes in the V1/V2 region of HIV-1 envelope glycoprotein (Env).
View Article and Find Full Text PDFOral Immunization with Recombinant Vaccinia Virus Prime and Intramuscular Protein Boost Provides Protection against Intrarectal Simian-Human Immunodeficiency Virus Challenge in Macaques.
Clin Vaccine Immunol
December 2015
Department of Pharmaceutics, University of Washington, Seattle, Washington, USA Washington National Primate Research Center, University of Washington, Seattle, Washington, USA
Human immunodeficiency virus type 1 (HIV-1) acquisition occurs predominantly through mucosal transmission. We hypothesized that greater mucosal immune responses and protective efficacy against mucosal HIV-1 infection may be achieved by prime-boost immunization at mucosal sites. We used a macaque model to determine the safety, immunogenicity, and protective efficacy of orally delivered, replication-competent but attenuated recombinant vaccinia viruses expressing full-length HIV-1 SF162 envelope (Env) or simian immunodeficiency virus (SIV) Gag-Pol proteins.
View Article and Find Full Text PDFThe 1918 Influenza Virus PB2 Protein Enhances Virulence through the Disruption of Inflammatory and Wnt-Mediated Signaling in Mice.
J Virol
December 2015
Department of Microbiology, University of Washington, Seattle, Washington, USA Washington National Primate Research Center, University of Washington, Seattle, Washington, USA
Unlabelled: The 1918-1919 influenza pandemic remains the single greatest infectious disease outbreak in the past century. Mouse and nonhuman primate infection models have shown that the 1918 virus induces overly aggressive innate and proinflammatory responses. To understand the response to viral infection and the role of individual 1918 genes on the host response to the 1918 virus, we examined reassortant avian viruses nearly identical to the pandemic 1918 virus (1918-like avian virus) carrying either the 1918 hemagglutinin (HA) or PB2 gene.
View Article and Find Full Text PDFConserved Role of an N-Linked Glycan on the Surface Antigen of Human Immunodeficiency Virus Type 1 Modulating Virus Sensitivity to Broadly Neutralizing Antibodies against the Receptor and Coreceptor Binding Sites.
J Virol
January 2016
Department of Microbiology, University of Washington, Seattle, Washington, USA Department of Pharmaceutics, University of Washington, Seattle, Washington, USA Washington National Primate Research Center, Seattle, Washington, USA
Unlabelled: HIV-1 establishes persistent infection in part due to its ability to evade host immune responses. Occlusion by glycans contributes to masking conserved sites that are targets for some broadly neutralizing antibodies (bNAbs). Previous work has shown that removal of a highly conserved potential N-linked glycan (PNLG) site at amino acid residue 197 (N7) on the surface antigen gp120 of HIV-1 increases neutralization sensitivity of the mutant virus to CD4 binding site (CD4bs)-directed antibodies compared to its wild-type (WT) counterpart.
View Article and Find Full Text PDFSuppressor of Cytokine Signaling 3 Is an Inducible Host Factor That Regulates Virus Egress during Ebola Virus Infection.
J Virol
October 2015
Department of Microbiology, School of Medicine, University of Washington, Seattle, Washington, USA Washington National Primate Research Center, Seattle, Washington, USA
Unlabelled: Ebola virus (EBOV) initially targets monocytes and macrophages, which can lead to the release of proinflammatory cytokines and chemokines. These inflammatory cytokines are thought to contribute to the development of circulatory shock seen in fatal EBOV infections. The VP40 matrix protein is a key viral structural protein that is critical for virion egress.
View Article and Find Full Text PDFNext-generation sequencing reveals a controlled immune response to Zaire Ebola virus challenge in cynomolgus macaques immunized with vesicular stomatitis virus expressing Zaire Ebola virus glycoprotein (VSVΔG/EBOVgp).
Clin Vaccine Immunol
March 2015
Department of Microbiology, University of Washington, Seattle, Washington, USA Washington National Primate Research Center, University of Washington, Seattle, Washington, USA
Article Synopsis
- - The study investigates a potential vaccine, VSVΔG/EBOVgp, derived from the vesicular stomatitis virus modified to express Ebola virus glycoprotein, aimed at combating the 2014 Ebola outbreak.
- - Researchers analyzed the immune response by examining mRNA sequences in PBMCs from macaques after vaccination and exposure to the Ebola virus.
- - Findings indicate a well-regulated immune response that adjusts as the virus is cleared, suggesting the vaccine's safety and effectiveness in prompting an appropriate immune reaction.
Delayed inflammatory and cell death responses are associated with reduced pathogenicity in Lujo virus-infected cynomolgus macaques.
J Virol
March 2015
Department of Microbiology, University of Washington, Seattle, Washington, USA Washington National Primate Research Center, Seattle, Washington, USA
Unlabelled: To identify host factors associated with arenavirus virulence, we used a cynomolgus macaque model to evaluate the pathogenesis of Lujo virus (LUJV), a recently emerged arenavirus that caused an outbreak of severe viral hemorrhagic fever in southern Africa. In contrast to human cases, LUJV caused mild, nonlethal illness in macaques. We then compared this to contrasting clinical outcomes during arenavirus infection, specifically to samples obtained from macaques infected with three highly pathogenic lines of Lassa virus (LASV), the causative agent of Lassa fever (LF).
View Article and Find Full Text PDFTissue-specific transcriptome sequencing analysis expands the non-human primate reference transcriptome resource (NHPRTR).
Nucleic Acids Res
January 2015
Department of Physiology and Biophysics, Weill Cornell Medical College, New York, NY 10065, USA Institute for Computational Biology (ICB), Weill Cornell Medical College, New York, NY 10065, USA Feil Family Brain and Mind Research Institute (BMRI), Weill Cornell Medical College, New York, NY 10065, USA
Article Synopsis
- The NHPRTR project is creating a large database of RNA-seq data from various non-human primates, including lemurs and hominids, to help understand their genetics.
- In its recent phase, NHPRTR added over 10 billion new RNA-seq data fragments from 11 selected primate species, covering around 15 different tissues per species.
- The updated resource provides high-quality expression data aligned with human genes, improving transcript annotations for macaques and aiding research in genome annotation and functional analysis across different primate species.
Host genetic diversity enables Ebola hemorrhagic fever pathogenesis and resistance.
Science
November 2014
Department of Microbiology, University of Washington, Seattle, WA, USA. Washington National Primate Research Center, Seattle, WA, USA.
Article Synopsis
- Existing mouse models fail to accurately represent the symptoms of lethal Ebola virus infection, making it difficult to study the disease's pathogenesis in typical laboratory settings.
- Researchers found distinct disease responses in mice from the Collaborative Cross panel, ranging from complete resistance to severe symptoms leading to death.
- The study suggests that genetic variations influence susceptibility to Ebola hemorrhagic fever, with specific alleles impacting inflammatory responses and immune cell behavior.
Multiple low-dose challenges in a rhesus macaque AIDS vaccine trial result in an evolving host response that affects protective outcome.
Clin Vaccine Immunol
December 2014
Department of Microbiology, University of Washington, Seattle, Washington, USA Washington National Primate Research Center, University of Washington, Seattle, Washington, USA
Using whole-blood transcriptional profiling, we investigated differences in the host response to vaccination and challenge in a rhesus macaque AIDS vaccine trial. Samples were collected from animals prior to and after vaccination with live, irradiated vaccine cells secreting the modified endoplasmic reticulum chaperone gp96-Ig loaded with simian immunodeficiency virus (SIV) peptides, either alone or in combination with a SIV-gp120 protein boost. Additional samples were collected following multiple low-dose rectal challenges with SIVmac251.
View Article and Find Full Text PDFHIV vaccine trial exploits a dual and central role for innate immunity.
J Virol
October 2014
Washington National Primate Research Center, Seattle, Washington, USA Department of Pharmaceutics, University of Washington, Seattle, Washington, USA
Limited understanding of correlates of protection from HIV transmission hinders development of an efficacious vaccine. D. J.
View Article and Find Full Text PDFDeep sequencing of HIV-infected cells: insights into nascent transcription and host-directed therapy.
J Virol
August 2014
Department of Microbiology, University of Washington, Seattle, Washington, USA Washington National Primate Research Center, Seattle, Washington, USA
Article Synopsis
- The study focuses on the differences in gene expression in HIV-1-infected CD4(+) T cells by comparing two types of RNA sequencing: Total RNAseq (which captures both polyadenylated and nonpolyadenylated RNAs) and mRNAseq (which only targets mature mRNAs).
- Researchers discovered that analyzing Total RNAseq yielded eight times more differentially expressed genes shortly after infection, highlighting the importance of examining nascent (newly transcribed) transcripts for understanding early transcriptional responses.
- The findings reveal a significant delay between transcription initiation and the production of mature mRNAs post-infection and suggest that exploring both kinds of RNA is crucial for identifying potential drug targets to combat HIV/AIDS.
Deep transcriptional sequencing of mucosal challenge compartment from rhesus macaques acutely infected with simian immunodeficiency virus implicates loss of cell adhesion preceding immune activation.
J Virol
July 2014
Department of Microbiology, University of Washington, Seattle, Washington, USA Washington National Primate Research Center, University of Washington, Seattle, Washington, USA
Article Synopsis
- The study explores how the mucosal immune system in rhesus macaques responds to simian immunodeficiency virus (SIV) following high-dose intrarectal inoculation, revealing insights into early viral replication and systemic infection during acute phases.
- At 3 days post-inoculation, a strong host transcriptional response was noted, but lacking antiviral immunity genes; instead, genes related to cell adhesion and cytoskeletal organization were differentially expressed.
- By 12 days post-inoculation, immune function genes were more prominent, indicating that mucosal integrity is compromised early, potentially exacerbating tissue damage and inflammation during peak viral loads.