2 results match your criteria: "USA. Electronic address: nhacohen@broadinstitute.org.[Affiliation]"
Classification and functional characterization of regulators of intracellular STING trafficking identified by genome-wide optical pooled screening.
Cell Syst
December 2024
Massachusetts Institute of Technology, Department of Health Sciences and Technology, Cambridge, MA, USA; Massachusetts General Hospital, Krantz Family Center for Cancer Research, Boston, MA, USA. Electronic address:
Stimulator of interferon genes (STING) traffics across intracellular compartments to trigger innate responses. Mutations in factors regulating this process lead to inflammatory disorders. To systematically identify factors involved in STING trafficking, we performed a genome-wide optical pooled screen (OPS).
View Article and Find Full Text PDFA Genome-wide CRISPR Screen in Primary Immune Cells to Dissect Regulatory Networks.
Cell
July 2015
Broad Institute of MIT and Harvard, Cambridge, MA 02142, USA; Department of Biology, Howard Hughes Medical Institute, Massachusetts Institute of Technology, Cambridge, MA 02140, USA. Electronic address:
Finding the components of cellular circuits and determining their functions systematically remains a major challenge in mammalian cells. Here, we introduced genome-wide pooled CRISPR-Cas9 libraries into dendritic cells (DCs) to identify genes that control the induction of tumor necrosis factor (Tnf) by bacterial lipopolysaccharide (LPS), a key process in the host response to pathogens, mediated by the Tlr4 pathway. We found many of the known regulators of Tlr4 signaling, as well as dozens of previously unknown candidates that we validated.
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