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Appl Environ Microbiol
February 2016
Department of Molecular Biology and Genetics, Cornell University, Ithaca, New York, USA Department of Microbiology, Cornell University, Ithaca, New York, USA Max Planck Institute for Developmental Biology, Tübingen, Germany
Metagenomes derived from environmental microbiota encode a vast diversity of protein homologs. How this diversity impacts protein function can be explored through selection assays aimed to optimize function. While artificially generated gene sequence pools are typically used in selection assays, their usage may be limited because of technical or ethical reasons.
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