3,046 results match your criteria: "USA M.Z.; University of North Texas System College of Pharmacy[Affiliation]"
Sci Rep
October 2023
Janssen Research and Development LLC, The Janssen Pharmaceutical Companies of Johnson & Johnson, Spring House, PA, USA.
Protein therapeutics are susceptible to clipping via enzymatic and nonenzymatic mechanisms that create neo-N-termini. Typically, neo-N-termini are identified by chemical derivatization of the N-terminal amine with (N-Succinimidyloxycarbonylmethyl)tris(2,4,6-trimethoxyphenyl)phosphonium bromide (TMPP) followed by proteolysis and mass spectrometric analysis. Detection of the TMPP-labeled peptide is achieved by mapping the peptide sequence to the product ion spectrum derived from collisional activation.
View Article and Find Full Text PDFJ Mass Spectrom
November 2023
Department of Chemistry and Biochemistry, Brigham Young University, Provo, Utah, 84602-1030, USA.
Curr Drug Metab
December 2023
School of Chinese Materia Medica, Tianjin University of Traditional Chinese Medicine, Tianjin, 301617, China.
Background: Isovitexin-2"-O-D-glucopyranoside (IVG) has been known to exhibit sedative and hypnotic effects. However, there is little understanding of the in vivo pharmacokinetics and tissue distribution of IVG.
Objective: This study aimed to investigate the pharmacokinetics and tissue distribution of IVG.
J Community Hosp Intern Med Perspect
May 2023
Division of Endocrinology, Diabetes and Metabolism, Johns Hopkins University School of Medicine, Baltimore, MD, USA.
We present a rare case of hydatidiform molar pregnancy, which led to the development of thyroid storm, followed by a rare complication of takotsubo cardiomyopathy in the setting of a COVID-19 infection. A 21-year-old female of 22 weeks gestational age presented with heavy vaginal blood loss, brown emesis, tachycardia, and lethargy. Through clinical presentation and ultrasound confirmation, a molar pregnancy was diagnosed.
View Article and Find Full Text PDFBiochem Pharmacol
December 2023
College of Pharmacy and Pharmaceutical Sciences, Washington State University, Spokane, WA 99202, USA. Electronic address:
Transporter-mediated drug-drug interactions (DDIs) are assessed using probe drugs and in vitro and in vivo models during drug development. The utility of endogenous metabolites as transporter biomarkers is emerging for prediction of DDIs during early phases of clinical trials. Endogenous metabolites such as pyridoxic acid and kynurenic acid have shown potential to predict DDIs mediated by organic anion transporters (OAT1 and OAT3).
View Article and Find Full Text PDFClin Transl Sci
December 2023
Genentech, Inc., South San Francisco, California, USA.
MethodsX
December 2023
Institute at Brown for Environment and Society, Brown University, Providence, RI 02912, USA.
Oxygen stable isotopes (i.e., O, O, O) of nitrite (NO) are useful for investigating chemical processes and sources contributing to this important environmental contaminant and nutrient.
View Article and Find Full Text PDFAnalyst
November 2023
Department of Chemistry, University of Connecticut, Storrs, Connecticut 06269, USA.
Angiotensin and kinin metabolic pathways are reported to be altered by many diseases, including COVID-19. Monitoring levels of these peptide metabolites is important for understanding mechanisms of disease processes. In this paper, we report dimethyl labeling of amines in peptides by addition of formaldehyde to samples and deutero-formaldehyde to internal standards to generate nearly identical isotopic standards with 4 / units larger per amine group than the corresponding analyte.
View Article and Find Full Text PDFGeochem Geophys Geosyst
February 2023
Department of Earth, Planetary, and Space Sciences, Department of Atmospheric and Oceanic Sciences, Center for Diverse Leadership in Science, Institute of the Environment and Sustainability, UCLA, Los Angeles, CA, USA.
Carbonate clumped isotope geochemistry has primarily focused on mass spectrometric determination of 47 CO for geothermometry, but theoretical calculations and recent experiments indicate paired analysis of the 47 (COO) and 48 (COO) isotopologues (referred to as and ) can be used to study non-equilibrium isotope fractionations and refine temperature estimates. We utilize 5,448 and 3,400 replicate measurements of carbonate samples and standards, and 183 and 195 replicate measurements of gas standards from 2015 to 2021 from a multi-year and multi-instrument data set to constrain and values for 27 samples and standards, including Devils Hole cave calcite, and study equilibrium -, -temperature, and -temperature relationships. We compare results to previously published findings and calculate equilibrium regressions based on data from multiple laboratories.
View Article and Find Full Text PDFCurr Biol
October 2023
Cold Spring Harbor Laboratory, 1 Bungtown Rd, Cold Spring Harbor, NY 11724, USA.
The activity of neurons in the auditory cortex is driven by both sounds and non-sensory context. To investigate the neuronal correlates of non-sensory context, we trained head-fixed mice to perform a two-alternative-choice auditory task in which either reward or stimulus expectation (prior) was manipulated in blocks. Using two-photon calcium imaging to record populations of single neurons in the auditory cortex, we found that both stimulus and reward expectation modulated the activity of these neurons.
View Article and Find Full Text PDFChem Sci
October 2023
Department of Chemistry, University of Pennsylvania Philadelphia PA 19104-6323 USA
Biogenic alkenes, such as isoprene and α-pinene, are the predominant source of volatile organic compounds (VOCs) emitted into the atmosphere. Atmospheric processing of alkenes reaction with ozone leads to formation of zwitterionic reactive intermediates with a carbonyl oxide functional group, known as Criegee intermediates (CIs). CIs are known to exhibit a strong absorption (π* ← π) in the near ultraviolet and visible (UV-vis) region due to the carbonyl oxide moiety.
View Article and Find Full Text PDFFood Funct
October 2023
Department of Food Science, The Pennsylvania State University, 332 Food Science Building, University Park, PA 16802, USA.
The green tea polyphenol, (-)-epigallocatechin-3-gallate (EGCG), has been studied for its potential positive health effects, but human and animal model studies have reported potential toxicity at high oral bolus doses. This study used liquid chromatography-mass spectrometry-based metabolomics to compare the urinary EGCG metabolite profile after administration of a single non-toxic (100 mg kg) or toxic (750 mg kg) oral bolus dose to male C57BL6/J mice to better understand how EGCG metabolism varies with dose. EGCG metabolites, including methyl, glucuronide, sulfate, and glucoside conjugates, were tentatively identified based on their mass to charge (/) ratio and fragment ion patterns.
View Article and Find Full Text PDFSci Total Environ
January 2024
Department of Soil, Water, and Ecosystem Science, University of Florida, Gainesville, FL 32611, USA.
Cell
September 2023
Laboratory of Single Cell Genomics and Population Dynamics, The Rockefeller University, New York, NY, USA. Electronic address:
Progenitor cells are critical in preserving organismal homeostasis, yet their diversity and dynamics in the aged brain remain underexplored. We introduced TrackerSci, a single-cell genomic method that combines newborn cell labeling and combinatorial indexing to characterize the transcriptome and chromatin landscape of proliferating progenitor cells in vivo. Using TrackerSci, we investigated the dynamics of newborn cells in mouse brains across various ages and in a mouse model of Alzheimer's disease.
View Article and Find Full Text PDFMolecules
September 2023
National Center for Natural Products Research, School of Pharmacy, University of Mississippi, University, MS 38677, USA.
Lavender ( Miller or Chaix) is an ethnopharmacological plant commonly known as English lavender. Linalool and linalyl acetate are putative phytoactives in lavender essential oil (LEO) derived from the flower heads. LEO has been used in aroma or massage therapy to reduce sleep disturbance and to mitigate anxiety.
View Article and Find Full Text PDFAntibiotics (Basel)
September 2023
Infectious Diseases Research Laboratory, Mayo Clinic, Rochester, MN 55905, USA.
Rapid detection of carbapenemase (KPC) in the species is desirable. The MALDI Biotyper MBT Subtyping Module (Bruker Daltonics) uses an algorithm that detects a peak at ~11,109 m/z corresponding to a protein encoded by the gene to detect KPC simultaneously with organism identification by a matrix-assisted laser desorption ionization-time-of-flight mass spectrometry (MALDI-ToF MS). Here, the subtyping module was evaluated using 795 clinical isolates, with whole genome sequences used to assess for and .
View Article and Find Full Text PDFAnal Bioanal Chem
November 2023
Functional Proteomics, Jena University Hospital, Am Klinikum 1, 07747, Jena, Germany.
Recent advances have rekindled the interest in ion mobility as an additional dimension of separation in mass spectrometry (MS)-based proteomics. Ion mobility separates ions according to their size and shape in the gas phase. Here, we set out to investigate the effect of 22 different post-translational modifications (PTMs) on the collision cross section (CCS) of peptides.
View Article and Find Full Text PDFIntroduction: It is critical to develop more inclusive Alzheimer's disease (AD) research protocols to ensure that historically excluded groups are included in preclinical research and have access to timely diagnosis and treatment. If validated in racialized groups, plasma AD biomarkers and measures of subtle cognitive dysfunction could provide avenues to expand diversity in preclinical AD research. We sought to evaluate the utility of two easily obtained, low-burden disease markers, plasma amyloid beta (Aβ)42/40, and intra-individual cognitive variability (IICV), to predict concurrent and longitudinal cognitive performance in a sample of Black adults.
View Article and Find Full Text PDFBiomed Chromatogr
January 2024
Department of Chemistry & Biochemistry, University of Wisconsin-, Milwaukee, WI, USA.
Despite aggressive treatment approaches, the overall survival of glioblastoma (GBM) patients remained poor with a strong need for more effective chemotherapeutic agents. A previous study has shown that ARN14988 is more cytotoxic to GBM cells compared to US Food and Drug Administration-approved temozolomide. This finding makes ARN14988 a desirable candidate for further pharmacological assessment.
View Article and Find Full Text PDFbioRxiv
September 2023
Cold Spring Harbor Laboratory, 1 Bungtown Rd, Cold Spring Harbor, NY 11724, USA.
The activity of neurons in the auditory cortex is driven by both sounds and non-sensory context. To investigate the neuronal correlates of non-sensory context, we trained head-fixed mice to perform a two-alternative choice auditory task in which either reward or stimulus expectation (prior) was manipulated in blocks. Using two-photon calcium imaging to record populations of single neurons in auditory cortex, we found that both stimulus and reward expectation modulated the activity of these neurons.
View Article and Find Full Text PDFEnviron Sci Pollut Res Int
October 2023
Sao Paulo State University Julio de Mesquita Filho, Institute of Chemistry, Araraquara, Sao Paulo, Brazil.
Anaerobic bioreactors are an efficient technology for the biodegradation of emerging contaminants in environmental matrices. In this work, a horizontal-flow anaerobic immobilized biomass (HAIB) bioreactor was used to remove caffeine (CAF), which is frequently found in various aqueous matrices. The acrylic bench top bioreactor, with dimensions of 100 × 5.
View Article and Find Full Text PDFJ Infect Dis
April 2024
Division of Infectious Diseases, Department of Medicine, Emory University School of Medicine, Atlanta, Georgia, USA.
Background: High-resolution metabolomics (HRM) is an innovative tool to study challenging infectious diseases like leprosy, where the pathogen cannot be grown with standard methods. Here, we use HRM to better understand associations between disease manifestations, nutrition, and host metabolism.
Methods: From 2018 to 2019, adults with leprosy and controls were recruited in Minas Gerais, Brazil.
Nat Rev Endocrinol
December 2023
Center for Translational Medicine and Pharmacology, Icahn School of Medicine at Mount Sinai, New York, NY, USA.
Traditional textbook physiology has ascribed unitary functions to hormones from the anterior and posterior pituitary gland, mainly in the regulation of effector hormone secretion from endocrine organs. However, the evolutionary biology of pituitary hormones and their receptors provides evidence for a broad range of functions in vertebrate physiology. Over the past decade, we and others have discovered that thyroid-stimulating hormone, follicle-stimulating hormone, adrenocorticotropic hormone, prolactin, oxytocin and arginine vasopressin act directly on somatic organs, including bone, adipose tissue and liver.
View Article and Find Full Text PDFForensic Sci Int
October 2023
Department of Forensic Science, Sam Houston State University, Huntsville, TX, USA. Electronic address:
Forensic laboratories are faced with an ever-expanding seized drug landscape including the increasing prevalence of novel psychoactive substances (NPS), such as synthetic cathinones, that have varying potencies and scheduling. This study demonstrates a combined gas chromatography-electron ionization-mass spectrometry (GC-EI-MS) and nuclear magnetic resonance (NMR) spectroscopy approach for the differentiation of N-butyl pentylone isomers based on distinct retention times, characteristic EI mass spectra, and NMR characterization. Retention time reproducibility was assessed from 60 replicate measurements for each isomer over the course of a month.
View Article and Find Full Text PDFEuropace
August 2023
Department of Cardiology, Heart Center, Amsterdam UMC location University of Amsterdam, Meibergdreef 9, 1105 AZ Amsterdam, The Netherlands.