13 results match your criteria: "USA [2] Jonsson Comprehensive Cancer Center[Affiliation]"
Cystatin E/M Suppresses Tumor Cell Growth through Cytoplasmic Retention of NF-κB.
Mol Cell Biol
June 2016
Division of General Surgery, Department of Surgery, VAGLAHS/David Geffen School of Medicine at UCLA, Los Angeles, California, USA Jonsson Comprehensive Cancer Center, UCLA, Los Angeles, California, USA
We and others have shown that the cystatin E/M gene is inactivated in primary human tumors, pointing to its role as a tumor suppressor gene. However, the molecular mechanism of tumor suppression is not yet understood. Using plasmid-directed cystatin E/M gene overexpression, a lentivirus-mediated tetracycline-inducible vector system, and human papillomavirus 16 (HPV 16) E6 and E7 gene-immortalized normal human epidermal keratinocytes, we demonstrated intracellular and non-cell-autonomous apoptotic growth inhibition of tumor cell lines and that growth inhibition is associated with cytoplasmic retention of NF-κB.
View Article and Find Full Text PDFIndividualizing liver transplant immunosuppression using a phenotypic personalized medicine platform.
Sci Transl Med
April 2016
Jonsson Comprehensive Cancer Center, University of California, Los Angeles, Los Angeles, CA 90095, USA. Division of Oral Biology and Medicine and the Jane and Jerry Weintraub Center for Reconstructive Biotechnology, School of Dentistry, University of California, Los Angeles, Los Angeles, CA 90095, USA. Department of Bioengineering, Henry Samueli School of Engineering and Applied Science, University of California, Los Angeles, Los Angeles, CA 90095, USA. California NanoSystems Institute, University of California, Los Angeles, Los Angeles, CA 90095, USA.
Posttransplant immunosuppressive drugs such as tacrolimus have narrow therapeutic ranges. Inter- and intraindividual variability in dosing requirements conventionally use physician-guided titrated drug administration, which results in frequent deviations from the target trough ranges, particularly during the critical postoperative phase. There is a clear need for personalized management of posttransplant regimens to prevent adverse events and allow the patient to be discharged sooner.
View Article and Find Full Text PDFLaryngeal Preservation in Glottic Cancer: A Comparison of Hospital Charges and Morbidity among Treatment Options.
Otolaryngol Head Neck Surg
August 2016
Department of Head and Neck Surgery, David Geffen School of Medicine at the University of California-Los Angeles, Los Angeles, California, USA Jonsson Comprehensive Cancer Center, David Geffen School of Medicine at the University of California-Los Angeles, Los Angeles, California, USA
Objective: When total laryngectomy is not required, organ preservation surgery or radiotherapy is considered the standard of care for primary glottic cancer. These accepted treatment options are available for early and advanced glottic cancers due to equivalent locoregional control and survival rates. However, in today's climate of accountable care, the financial burden of treatment choices continues to increase in significance.
View Article and Find Full Text PDFSignificance of Tumor Stage in Sinonasal Undifferentiated Carcinoma Survival: A Population-Based Analysis.
Otolaryngol Head Neck Surg
April 2016
Department of Head and Neck Surgery, David Geffen School of Medicine at UCLA, Los Angeles, California, USA Jonsson Comprehensive Cancer Center, UCLA Medical Center, Los Angeles, California, USA UCLA Head and Neck Cancer Program, UCLA Medical Center, Los Angeles, California, USA.
Objective: To describe the incidence and determinants of survival of patients with sinonasal undifferentiated carcinoma (SNUC) from 1973 to 2011 using the SEER database (Surveillance, Epidemiology, and End Results), with consideration of tumor stage based on the Kadish system.
Study Design: Retrospective database analysis.
Setting: Academic medical center.
Pulp-dentin Regeneration: Current State and Future Prospects.
J Dent Res
November 2015
School of Dentistry, UCLA, Los Angeles, CA, USA Jonsson Comprehensive Cancer Center, UCLA, Los Angeles, CA, USA
The goal of regenerative endodontics is to reinstate normal pulp function in necrotic and infected teeth that would result in reestablishment of protective functions, including innate pulp immunity, pulp repair through mineralization, and pulp sensibility. In the unique microenvironment of the dental pulp, the triad of tissue engineering would require infection control, biomaterials, and stem cells. Although revascularization is successful in resolving apical periodontitis, multiple studies suggest that it alone does not support pulp-dentin regeneration.
View Article and Find Full Text PDFA fully human scFv phage display library for rapid antibody fragment reformatting.
Protein Eng Des Sel
October 2015
Crump Institute for Molecular Imaging, Department of Molecular and Medical Pharmacology, David Geffen School of Medicine at UCLA, 570 Westwood Plaza, Box 951770, Los Angeles, CA 90095, USA Jonsson Comprehensive Cancer Center at UCLA, Los Angeles, CA 90095, USA
Phage display libraries of human single-chain variable fragments (scFvs) are a reliable source of fully human antibodies for scientific and clinical applications. Frequently, scFvs form the basis of larger, bivalent formats to increase valency and avidity. A small and versatile bivalent antibody fragment is the diabody, a cross-paired scFv dimer (∼55 kDa).
View Article and Find Full Text PDFAutocrine VEGF maintains endothelial survival through regulation of metabolism and autophagy.
J Cell Sci
June 2015
Department of Molecular, Cell and Developmental Biology, University of California, Los Angeles, CA 90024, USA Molecular Biology Institute, University of California, Los Angeles, CA 90024, USA Jonsson Comprehensive Cancer Center, University of California, Los Angeles, CA 90024, USA
Autocrine VEGF is necessary for endothelial survival, although the cellular mechanisms supporting this function are unknown. Here, we show that--even after full differentiation and maturation--continuous expression of VEGF by endothelial cells is needed to sustain vascular integrity and cellular viability. Depletion of VEGF from the endothelium results in mitochondria fragmentation and suppression of glucose metabolism, leading to increased autophagy that contributes to cell death.
View Article and Find Full Text PDFNovel role for cyclophilin A in regulation of chondrogenic commitment and endochondral ossification.
Mol Cell Biol
June 2015
Dental and Craniofacial Research Institute and Division of Oral Biology, School of Dentistry, University of California, Los Angeles, Los Angeles, California, USA Jonsson Comprehensive Cancer Center and Division of Surgical Oncology, School of Medicine, University of California, Los Angeles, Los Angeles, California, USA
Recent studies showed that cyclophilin A (CypA) promotes NF-κB/p65 nuclear translocation, resulting in enhanced NF-κB activity and altered expression of its target genes, such as the Sox9 transcriptional factor, which plays a critical role in chondrogenic differentiation and endochondral ossification. In this report, we unveil the role of CypA in signal-induced chondrogenic differentiation and endochondral ossification. Expression levels of the chondrogenic differentiation markers and transcriptional regulators Sox9 and Runx2 were all significantly lower in CypA knockdown chondrogenic cells than in wild-type cells, indicating that CypA plays a functional role in chondrogenic differentiation.
View Article and Find Full Text PDFImproved antitumor activity of immunotherapy with BRAF and MEK inhibitors in BRAF(V600E) melanoma.
Sci Transl Med
March 2015
Division of Hematology-Oncology, Department of Medicine, University of California, Los Angeles (UCLA), Los Angeles, CA 90095, USA. Department of Molecular and Medical Pharmacology, UCLA, Los Angeles, CA 90095, USA. Jonsson Comprehensive Cancer Center, UCLA, Los Angeles, CA 90095, USA. Division of Surgical Oncology, Department of Surgery, UCLA, Los Angeles, CA 90095, USA.
Combining immunotherapy and BRAF targeted therapy may result in improved antitumor activity with the high response rates of targeted therapy and the durability of responses with immunotherapy. However, the first clinical trial testing the combination of the BRAF inhibitor vemurafenib and the CTLA4 antibody ipilimumab was terminated early because of substantial liver toxicities. MEK [MAPK (mitogen-activated protein kinase) kinase] inhibitors can potentiate the MAPK inhibition in BRAF mutant cells while potentially alleviating the unwanted paradoxical MAPK activation in BRAF wild-type cells that lead to side effects when using BRAF inhibitors alone.
View Article and Find Full Text PDFThe Sm protein methyltransferase PRMT5 is not required for primordial germ cell specification in mice.
EMBO J
March 2015
Department of Molecular Cell and Developmental Biology, University of California Los Angeles, Los Angeles, CA, USA Eli and Edythe Broad Center of Regenerative Medicine and Stem Cell Research, University of California Los Angeles, Los Angeles, CA, USA Molecular Biology Institute, University of California Los Angeles, Los Angeles, CA, USA Jonsson Comprehensive Cancer Center, University of California Los Angeles, Los Angeles, CA, USA
PRMT5 is a type II protein arginine methyltransferase with roles in stem cell biology, reprograming, cancer and neurogenesis. During embryogenesis in the mouse, it was hypothesized that PRMT5 functions with the master germline determinant BLIMP1 to promote primordial germ cell (PGC) specification. Using a Blimp1-Cre germline conditional knockout, we discovered that Prmt5 has no major role in murine germline specification, or the first global epigenetic reprograming event involving depletion of cytosine methylation from DNA and histone H3 lysine 9 dimethylation from chromatin.
View Article and Find Full Text PDFPluripotent stem cell energy metabolism: an update.
EMBO J
January 2015
Molecular Biology Institute, University of California, Los Angeles, CA, USA Department of Pathology and Laboratory Medicine, University of California, Los Angeles, CA, USA Department of Bioengineering, University of California, Los Angeles, CA, USA Department of Pediatrics, University of California, Los Angeles, CA, USA California NanoSystems Institute, University of California, Los Angeles, CA, USA Jonsson Comprehensive Cancer Center, University of California, Los Angeles, CA, USA Broad Center of Regenerative Medicine and Stem Cell Research, University of California, Los Angeles, CA, USA
Recent studies link changes in energy metabolism with the fate of pluripotent stem cells (PSCs). Safe use of PSC derivatives in regenerative medicine requires an enhanced understanding and control of factors that optimize in vitro reprogramming and differentiation protocols. Relative shifts in metabolism from naïve through "primed" pluripotent states to lineage-directed differentiation place variable demands on mitochondrial biogenesis and function for cell types with distinct energetic and biosynthetic requirements.
View Article and Find Full Text PDFNanomedicine for global health.
J Lab Autom
December 2014
Division of Oral Biology and Medicine, UCLA School of Dentistry, Los Angeles, CA, USA Department of Bioengineering, UCLA School of Engineering and Applied Science, Los Angeles, CA, USA Jonsson Comprehensive Cancer Center, UCLA, Los Angeles, CA, USA California NanoSystems Institute, UCLA, Los Angeles, CA, USA
Despite modern advances, a broad range of disorders such as cancer and infectious diseases continually afflict the global population. Novel therapeutics are continuously being explored to address these challenges. Therefore, scalable, effective, and safe therapies that are readily accessible to third-world countries are of major interest.
View Article and Find Full Text PDFA cytokine-delivering polymer is effective in reducing tumor burden in a head and neck squamous cell carcinoma murine model.
Otolaryngol Head Neck Surg
September 2014
Department of Head and Neck Surgery, University of California, Los Angeles, Los Angeles, California, USA UCLA Head and Neck Cancer Program, University of California, Los Angeles, Los Angeles, California, USA Jonsson Comprehensive Cancer Center, University of California, Los Angeles, Los Angeles, California, USA
Objective: This study aimed to evaluate the therapeutic efficacy of a novel polymer platform delivering cisplatin and cytokines in the treatment of head and neck squamous cell carcinoma (HNSCC).
Study Design: In vivo study.
Setting: Academic research laboratory.