Lyophilization of ASFV vaccine candidate ASFV-G-ΔI177L offers long term stability.

For over a century African swine fever (ASF) has been causing outbreaks leading to devastating losses for the swine industry. The current pandemic of ASF has shown no signs of stopping and continues to spread causing outbreaks in additional countries. Currently control relies mostly on culling infected farms, and strict biosecurity procedures.

Factors affecting fatigue progression in multiple sclerosis patients.

Fatigue is one of the most prevalent and disabling symptoms among patients with MS, but there is limited research investigating the longitudinal determinants of fatigue progression. This study aims to identify the sociodemographic, behavioral and clinical characteristics, and therapeutic regimens that are correlated with worsening fatigue over time in patients diagnosed with MS. This is a retrospective chart review of 483 patients.

A harmonized river-ocean coupled database for the northern Gulf of Mexico.

The northern Gulf of Mexico (nGoM) receives water from over 50 rivers which are highly influenced by humans and include the largest river in the United States, the Mississippi River. To support large-scale data-driven research centered on the dynamic river-ocean system in the region, this study consolidated hydrogeochemical river and ocean data from across the nGoM. In particular, we harmonized 35 chemical solute parameters from 54 rivers and incorporated river discharge data to derive daily solute concentration and flux estimates throughout the nGoM.

Study of the litter in the urban environment as primary and secondary microplastics sources.

Microplastic is one of the most important environmental challenges of recent decades. Although the abundance of microplastics in water sources and water bodies such as the marine were investigated in many studies, knowing the sources of microplastics requires more studies. In this study, litter was investigated as one of the challenges of urban management and the sources of primary microplastic and secondary microplastic in the urban environment.

Heart rate dynamics for cognitive load estimation in a driving simulation task.

Cognitive load (CL) is one of the leading factors moderating states and performance among drivers. Heavily increased CL may contribute to the development of mental stress. Averaged heart rate (HR) and heart rate variability (HRV) indices are shown to reflect CL levels in different tasks.

Elucidating governing factors of PFAS removal by polyamide membranes using machine learning and molecular simulations.

Per- and polyfluoroalkyl substances (PFASs) have recently garnered considerable concerns regarding their impacts on human and ecological health. Despite the important roles of polyamide membranes in remediating PFASs-contaminated water, the governing factors influencing PFAS transport across these membranes remain elusive. In this study, we investigate PFAS rejection by polyamide membranes using two machine learning (ML) models, namely XGBoost and multimodal transformer models.

Analysis of bulletproof performance of structurally optimized ceramic composite armor through numerical simulation and live fire test.

This work aims to address key issues in the ballistic performance of ceramic-based composite armor, particularly at the joints of spliced ​​bulletproof panels. The edge structure of C/C-SiC ceramic plates and ultra-high molecular weight polyethylene is redesigned to superimpose the joint areas. These structurally optimized composite pads are examined by numerical simulation of impact dynamics to understand their anti-penetration performance whose accuracy is then validated by live fire tests.

Imagine going left versus imagine going right: whole-body motion on the lateral axis.

Unlike the conventional, embodied, and embrained whole-body movements in the sagittal forward and vertical axes, movements in the lateral/transversal axis cannot be unequivocally grounded, embodied, or embrained. When considering motor imagery for left and right directions, it is  assumed that participants have underdeveloped representations due to a lack of familiarity with moving along the lateral axis. In the current study, a 32 electroencephalography (EEG) system was used to identify the oscillatory neural signature linked with lateral axis motor imagery.

The effects of chicken hydrolyzed proteins in extruded diets on plasma and fecal metabolic profiles in adult dogs.

Research has shown various hydrolyzed proteins possessed beneficial physiological functions; however, the mechanism of how hydrolysates influence metabolism is unclear. Therefore, the current study aimed to examine the effects of different sources of protein hydrolysates, being the main dietary protein source in extruded diets, on metabolism in healthy adult dogs. Three complete and balanced extruded canine diets were formulated: control chicken meal diet (CONd), chicken liver and heart hydrolysate diet (CLHd), mechanically separated chicken hydrolysate diet (CHd).

Safety and tolerability of the M2 muscarinic acetylcholine receptor modulator BAY 2413555 in heart failure with reduced ejection fraction in the REMOTE-HF study.

BAY 2413555 is a novel selective and reversible positive allosteric modulator of the type 2 muscarinic acetylcholine (M2) receptor, aimed at enhancing parasympathetic signaling and restoring cardiac autonomic balance for the treatment of heart failure (HF). This study tested the safety, tolerability and pharmacokinetics of this novel therapeutic option. REMOTE-HF was a multicenter, double-blind, randomized, placebo-controlled, phase Ib dose-titration study with two active arms.

A simple model of the rheological curve of HPAM solutions at different temperatures.

The oil and gas industry faces two significant challenges, including rising global temperatures and depletion of reserves. Enhanced recovery techniques such as polymer flooding have positioned themselves as an alternative that attracts international attention thanks to increased recovery factors with low emissions. However, existing physical models need further refinement to improve predictive accuracy and prevent design failures in polymer flooding projects.

Recent methane surges reveal heightened emissions from tropical inundated areas.

Record breaking atmospheric methane growth rates were observed in 2020 and 2021 (15.2±0.5 and 17.

Age-dependent regulation of axoglial interactions and behavior by oligodendrocyte AnkyrinG.

The bipolar disorder (BD) risk gene ANK3 encodes the scaffolding protein AnkyrinG (AnkG). In neurons, AnkG regulates polarity and ion channel clustering at axon initial segments and nodes of Ranvier. Disruption of neuronal AnkG causes BD-like phenotypes in mice.

Experimental assessment and analysis of mass transport limiting current density in water vapor-fed polymer electrolyte membrane electrolyzers.

Polymer electrolyte membrane water electrolyzers (PEMWEs) are a critical technology for efficient hydrogen production to decarbonize fuels and industrial feedstocks. To make hydrogen cost-effective, the overpotentials across the cell need to be decreased and platinum-group metal loading reduced. One overpotential that needs to be better understood is due to mass transport limitations from bubble formation within the porous transport layer (PTL) and anode catalyst layer (ACL), which can lead to a reduction in performance at typical operating current densities.

AI allows pre-screening of FGFR3 mutational status using routine histology slides of muscle-invasive bladder cancer.

Pathogenic activating mutations in the fibroblast growth factor receptor 3 (FGFR3) drive disease maintenance and progression in urothelial cancer. 10-15% of muscle-invasive and metastatic urothelial cancer (MIBC/mUC) are FGFR3-mutant. Selective targeting of FGFR3 hotspot mutations with tyrosine kinase inhibitors (e.

Heat stable and intrinsically sterile liquid protein formulations.

Over 80% of biologic drugs, and 90% of vaccines, require temperature-controlled conditions throughout the supply chain to minimize thermal inactivation and contamination. This cold chain is costly, requires stringent oversight, and is impractical in remote environments. Here, we report chemical dispersants that non-covalently solvate proteins within fluorous liquids to alter their thermodynamic equilibrium and reduce conformational flexibility.

Multivariate stochastic modeling for transcriptional dynamics with cell-specific latent time using SDEvelo.

Recently, RNA velocity has driven a paradigmatic change in single-cell RNA sequencing (scRNA-seq) studies, allowing the reconstruction and prediction of directed trajectories in cell differentiation and state transitions. Most existing methods of dynamic modeling use ordinary differential equations (ODE) for individual genes without applying multivariate approaches. However, this modeling strategy inadequately captures the intrinsically stochastic nature of transcriptional dynamics governed by a cell-specific latent time across multiple genes, potentially leading to erroneous results.

Biophysical effects of croplands on land surface temperature.

Converting natural vegetation to croplands alters the local land surface energy budget. Here, we use two decades of satellite data and a physics-based framework to analyse the biophysical mechanisms by which croplands influence daily mean land surface temperature (LST). Globally, 60% of croplands exhibit an annual warming effect, while 40% have a cooling effect compared to their surrounding natural ecosystems.

Defects in hair cells disrupt the development of auditory peripheral circuitry.

Deafness is the most common form of sensory impairment in humans and frequently caused by defects in hair cells of the inner ear. Here we demonstrate that in male mice which model recessive non-syndromic deafness (DFNB6), inactivation of Tmie in hair cells disrupts gene expression in the neurons that innervate them. This includes genes regulating axonal pathfinding and synaptogenesis, two processes that are disrupted in the inner ear of the mutant mice.

Oxidized phospholipid and transcriptomic signatures of THC-related vaping associated lung injury.

E-cigarette/vaping-associated lung injury (EVALI) is strongly associated with vitamin E acetate and often occurs with concomitant tetrahydrocannabinol (THC) use. To uncover pathways associated with EVALI, we examined cytokines, transcriptomic signatures, and lipidomic profiles in bronchoalveolar lavage fluid (BALF) from THC-EVALI patients. At a single center, we prospectively enrolled mechanically ventilated patients with EVALI from THC-containing products (N = 4) and patients with non-vaping acute lung injury and airway controls (N = 5).

Future increase in compound soil drought-heat extremes exacerbated by vegetation greening.

Compound soil drought and heat extremes are expected to occur more frequently with global warming, causing wide-ranging socio-ecological repercussions. Vegetation modulates air temperature and soil moisture through biophysical processes, thereby influencing the occurrence of such extremes. Global vegetation cover is broadly expected to increase under climate change, but it remains unclear whether vegetation greening will alleviate or aggravate future increases in compound soil drought-heat events.

INSIHGT: an accessible multi-scale, multi-modal 3D spatial biology platform.

Biological systems are complex, encompassing intertwined spatial, molecular and functional features. However, methodological constraints limit the completeness of information that can be extracted. Here, we report the development of INSIHGT, a non-destructive, accessible three-dimensional (3D) spatial biology method utilizing superchaotropes and host-guest chemistry to achieve homogeneous, deep penetration of macromolecular probes up to centimeter scales, providing reliable semi-quantitative signals throughout the tissue volume.

Bayesian modeling of HFC production pipeline suggests growth in unreported CFC by-product and feedstock production.

Observationally-derived emissions of ozone depleting substances must be scrutinized to maintain the progress made by the Montreal Protocol in protecting the stratospheric ozone layer. Recent observations of three chlorofluorocarbons (CFCs), CFC-113, CFC-114, and CFC-115, suggest that emissions of these compounds have not decreased as expected given global reporting of their production. These emissions have been associated with hydrofluorocarbon (HFC) production, which can require CFCs as feedstocks or generate CFCs as by-products, yet emissions from these pathways have not been rigorously quantified.

The Cryo-EM structure of human CD163 bound to haptoglobin-hemoglobin reveals molecular mechanisms of hemoglobin scavenging.

CD163, a macrophage-specific receptor, plays a critical role in scavenging hemoglobin released during hemolysis, protecting against oxidative effects of heme iron. In the bloodstream, hemoglobin is bound by haptoglobin, leading to its immediate endocytosis by CD163. While haptoglobin's structure and function are well understood, CD163's structure and its interaction with the haptoglobin-hemoglobin complex have remained elusive.

Cerebellar-driven cortical dynamics can enable task acquisition, switching and consolidation.

The brain must maintain a stable world model while rapidly adapting to the environment, but the underlying mechanisms are not known. Here, we posit that cortico-cerebellar loops play a key role in this process. We introduce a computational model of cerebellar networks that learn to drive cortical networks with task-outcome predictions.