5,608,306 results match your criteria: "USA; Englander Institute for Precision Medicine[Affiliation]"

Using eddy covariance data to detect nuclear reactor operational status.

J Environ Manage

December 2024

Nuclear and Engineering Nonproliferation Division, Los Alamos National Laboratory, Los Alamos, NM, 87545, USA.

Monitoring nuclear reactor operations is vital for nuclear safeguards as it ensures that reactors are in compliance with international legal agreements. Validating nuclear facilities and activities, including potential clandestine activities, is currently accomplished by using remotely sensed data from satellites and aircrafts and on-site sampling. However, these techniques are temporally-limited as sampling and interpretation of environmental releases frequently involve labor-intensive, on-site collections.

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The complex life cycle traits of amphibians make them especially sensitive to environmental change, and their ongoing conservation requires the maintenance of suitable habitat that accounts for such life cycle characteristics which may impacted by local environmental dynamics arising from climate change and human disturbance. Many existing studies on amphibian habitats disregard this important issue, leading to uncertainty in managing critical habitats. The application of appropriate conservation practices is therefore constrained by the fact that the major factors influencing amphibian habitats, and their spatio-temporal dynamics at different life stages, are poorly understood.

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Introduction: Antibody-drug conjugates (ADCs) trastuzumab-deruxtecan (T-DXd) and sacituzumab-govitecan (SG) provided significant progression-free survival (PFS) and overall survival (OS) improvements over chemotherapy (CT) in pretreated hormone receptor-positive (HR+) and triple-negative (TN)/HER2-low metastatic breast cancer (MBC). However, no direct comparison between the two exists, nor with the more recent datopotamab-deruxtecan (Dato-DXd).

Methods: We conducted a network meta-analysis (NMA) to compare efficacy and safety of T-DXd and SG in CT-pretreated HR+ and TN/HER2-low MBC and assess their benefit over standard CT, exploring also a comparison with Dato-DXd.

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Association of trimethylamine N-oxide and metabolites with kidney function decline in patients with chronic kidney disease.

Clin Nutr

December 2024

Division of Nephrology, Department of Medicine, Taipei Tzu Chi Hospital, Buddhist Tzu Chi Medical Foundation, and School of Medicine, Tzu Chi University, Hualien, Taiwan. Electronic address:

Background: Trimethylamine N-oxide (TMAO) is a gut microbial metabolite derived from dietary l-carnitine and choline. High plasma TMAO levels are associated with cardiovascular disease and overall mortality, but little is known about the associations of TMAO and related metabolites with the risk of kidney function decline among patients with chronic kidney disease (CKD).

Methods: We prospectively followed 152 nondialysis patients with CKD stages 3-5 and measured plasma TMAO and related metabolites (trimethylamine [TMA], choline, carnitine, and γ-butyrobetaine) via liquid chromatography‒mass spectrometry.

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Corrigendum to "Role of the ventral tegmental area in general anesthesia" [986(2025) 177145].

Eur J Pharmacol

December 2024

Key Laboratory of Anesthesiology and Resuscitation (Huazhong University of Science and Technology), Ministry of Education, Wuhan, 430048, China; Department of Anesthesiology, West China Hospital, Sichuan University, Chengdu, 610041, China.

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Astrocyte regulation of critical period plasticity across neural circuits.

Curr Opin Neurobiol

December 2024

Department of Pathology and Immunology, Washington University School of Medicine, Saint Louis, MO 63110, USA; Department of Developmental Biology, Washington University School of Medicine, Saint Louis, MO 63110, USA; Brain Immunology and Glia Center, Washington University School of Medicine, Saint Louis, MO 63110, USA. Electronic address:

Critical periods are brief windows of heightened neural circuit plasticity that allow circuits to permanently reset their structure and function to facilitate robust organismal behavior. Understanding the cellular and molecular mechanisms that instruct critical period timing is of broad clinical interest, as altered developmental plasticity is linked to multiple neurodevelopmental disorders. While intrinsic, neuronal mechanisms shape both neural circuit remodeling and critical period timing, recent data indicate that signaling from astrocytes and surrounding glia can both promote and limit critical period plasticity.

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Just the Tip of the Iceberg: a Brief Report of the Tip-of-the-Tongue Score as an Embedded Validity Indicator for the Children's Auditory and Visual Naming Tests.

Arch Clin Neuropsychol

December 2024

Departments of Psychiatry, Pediatrics, and The Dartmouth Institute for Health Policy and Clinical Practice, Geisel School of Medicine at Dartmouth, Hanover, NH, USA.

Objective: Explore the tip-of-the-tongue (TOT) scores from the Children's Auditory and Visual Naming Tests (cANT, cVNT) as embedded validity indicators (EVIs).

Method: A retrospective design of 98 consecutively referred youth aged 6-15 years (M = 11.28, SD = 2.

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Over the past two decades, new agents for multiple myeloma (MM) have significantly improved patient outcomes, particularly for those with standard-risk disease, who now have a median overall survival of over a decade. However, this benefit is less pronounced in high-risk and ultra-high-risk MM, where median survival ranges from 3 to 5 years. The definition of HRMM continues to evolve and is driven by the genomic features, disease burden, and medical comorbidities.

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Phytochemistry and nutraceutical potential of Ceylon Cinnamomum species native to Sri Lanka.

Nat Prod Res

December 2024

Interdisciplinary Centre for Innovations in Biotechnology and Neuroscience, Faculty of Medical Sciences, University of Sri Jayewardenepura, Sri Lanka.

Cinnamon is a spice that is renowned for its several medicinal and cosmetic benefits. The research study examined the essential oil content, antioxidant, and anti-inflammatory properties of seven species native to Sri Lanka. Cinnamon bark and leaf samples were used to extract essential oils, methanol, and hexane.

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Pathology discovered in the "first patient" can be the silent teacher of self-directed learning.

Clin Anat

December 2024

Department of Structural and Cellular Biology, School of Medicine, Tulane University, New Orleans, Louisiana, USA.

Pathology found during cadaveric dissection has been used to model integrative teaching methods for medical students at several institutions. Recent evidence has shown that pathology found during dissection can be used in the design of self-directed learning (SDL) activities with standards that are difficult to meet. This study presents a novel method for providing formative feedback, one of the most challenging aspects for LCME accreditation of SDL activities.

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Aims: We explored the prevalence of disordered eating behaviours (DEBs) and attitudes among older adults with type 1 diabetes (T1D) and associations with demographic and clinical variables.

Methods: Adults aged ≥65 years with T1D from a university-affiliated hospital system completed an electronic survey (September to November 2023) including the Diabetes Eating Problem Survey-Revised (DEPS-R). Clinical data were extracted from medical records.

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Background: U.S. FDA's Center for Biologics Evaluation and Research (CBER) Biologics Effectiveness and Safety (BEST) Initiative leverages large electronic health records and administrative claims data to conduct active surveillance for CBER-regulated products.

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Ascariasis, caused by the parasitic roundworm Ascaris lumbricoides, is a major public health issue, especially in developing nations such as Pakistan. It impacts millions, causing significant morbidity through starvation, stunted growth in children, and a variety of gastrointestinal issues. The taxonomy of the Ascaris genus, notably the distinction between A.

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Plant vacuoles are essential organelles that respond to developmental and environmental signals. Here, we present a protocol for staining the vacuole lumen in Arabidopsis root cells, enabling precise visualization of vacuolar dynamics. We describe steps for preparing plant material and staining with commonly used fluorescent dyes.

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The N-degron pathway mediates the autophagic degradation of cytosolic mitochondrial DNA during sterile innate immune responses.

Cell Rep

December 2024

Cellular Degradation Biology Center and Department of Biomedical Sciences, College of Medicine, Seoul National University, Seoul 03080, Republic of Korea; Convergence Research Center for Dementia, Seoul National University Medical Research Center, Seoul 110-799, Republic of Korea; AUTOTAC Bio, Inc., Changkkyunggung-ro 254, Jongno-gu, Seoul 03077, Republic of Korea; Ischemic/Hypoxic Disease Institute, College of Medicine, Seoul National University, Seoul 110-799, Republic of Korea. Electronic address:

The human body reacts to tissue damage by generating damage-associated molecular patterns (DAMPs) that activate sterile immune responses. To date, little is known about how DAMPs are removed to avoid excessive immune responses. Here, we show that proteasomal dysfunction induces the release of mitochondrial DNA (mtDNA) as a DAMP that activates the cyclic GMP-AMP synthase (cGAS)-stimulator of interferon gene (STING) pathway and is subsequently degraded through the N-degron pathway.

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Increased nuclear factor I-mediated chromatin access drives transition to androgen receptor splice variant dependence in prostate cancer.

Cell Rep

December 2024

Department of Medicine and Cancer Center, Beth Israel Deaconess Medical Center, Harvard Medical School, Boston, MA 02115, USA. Electronic address:

Androgen receptor (AR) splice variants, of which ARv7 is the most common, are increased in castration-resistant prostate cancer, but the extent to which they drive AR activity is unclear. We generated a subline of VCaP cells (VCaP16) that is resistant to the AR inhibitor enzalutamide (ENZ). AR activity in VCaP16 is driven by ARv7, independently of full-length AR (ARfl), and its cistrome and transcriptome mirror those of ARfl in VCaP cells.

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Transcriptional profile of the rat cardiovascular system at single-cell resolution.

Cell Rep

December 2024

Precision Cardiology Laboratory, The Broad Institute, Cambridge, MA 02142, USA; Cardiovascular Research Center, Massachusetts General Hospital, Boston, MA 02114, USA; Cardiology Division, Massachusetts General Hospital, Boston, MA 02114, USA. Electronic address:

We sought to characterize cellular composition across the cardiovascular system of the healthy Wistar rat, an important model in preclinical cardiovascular research. We performed single-nucleus RNA sequencing (snRNA-seq) in 78 samples in 10 distinct regions, including the four chambers of the heart, ventricular septum, sinoatrial node, atrioventricular node, aorta, pulmonary artery, and pulmonary veins, which produced 505,835 nuclei. We identified 26 distinct cell types and additional subtypes, with different cellular composition across cardiac regions and tissue-specific transcription for each cell type.

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Genetic variants in TMEM106B, coding for a transmembrane protein of unknown function, have been identified as critical genetic modulators in various neurodegenerative diseases with a strong effect in patients with frontotemporal degeneration. The luminal domain of TMEM106B can form amyloid-like fibrils upon proteolysis. Whether this luminal domain is generated under physiological conditions and which protease(s) are involved in shedding remain unclear.

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Contextual modulation emerges by integrating feedforward and feedback processing in mouse visual cortex.

Cell Rep

December 2024

Center for Theoretical Neuroscience and Mortimer B Zuckerman Mind Brain Behavior Institute, Columbia University, New York City, NY 10027, USA; Department of Neuroscience, Swartz Program in Theoretical Neuroscience, Kavli Institute for Brain Science, College of Physicians and Surgeons and Mortimer B. Zuckerman Mind Brain Behavior Institute, Columbia University, New York City, NY 10027, USA.

Sensory systems use context to infer meaning. Accordingly, context profoundly influences neural responses to sensory stimuli. However, a cohesive understanding of the circuit mechanisms governing contextual effects across different stimulus conditions is still lacking.

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Occupational therapy's whole-person approach is well-suited to address the complex needs of children and youth with fetal alcohol spectrum disorder (FASD). However, literature regarding best practices for occupational therapy practitioners working with this population is lacking. This article delineates the role and scope of occupational therapy practice for children and youth with FASD, focusing on holistic and strengths-based approaches.

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Background: Whereas restoration of fecal consistency after treatment with clioquinol for chronic diarrhea and free fecal water syndrome has been attributed to its antiprotozoal properties, actions of clioquinol on the colonic bacterial microbiota have not been investigated.

Objectives: Characterize the dynamics of fecal microbial diversity before, during, and after PO administration of clioquinol to healthy horses.

Study Design: Experimental prospective cohort study using a single horse group.

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[Donor DNA Modification with Cas9 Targeting Sites Improves the Efficiency of MTC34 Knock-in into the CXCR4 Locus].

Mol Biol (Mosk)

December 2024

Center of Precision Genome Editing and Genetic Technologies for Biomedicine, Institute of Gene Biology, Russian Academy of Sciences, Moscow, 119334 Russia.

To successfully apply the genome editing technology using the CRISPR/Cas9 system in the clinic, it is necessary to achieve a high efficiency of knock-in, which is insertion of a genetic construct into a given locus of the target cell genome. One of the approaches to increase the efficiency of knock-in is to modify donor DNA with the same Cas9 targeting sites (CTS) that are used to induce double-strand breaks (DSBs) in the cell genome (the double-cut donor method). Another approach is based on introducing truncated CTS (tCTS), including a PAM site and 16 proximal nucleotides, into the donor DNA.

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[Methods to Increase the Efficiency of Knock-in of a Construct Encoding the HIV-1 Fusion Inhibitor, MT-C34 Peptide, into the CXCR4 Locus in the CEM/R5 T Cell Line].

Mol Biol (Mosk)

December 2024

Center of Precision Genome Editing and Genetic Technologies for Biomedicine, Institute of Gene Biology, Russian Academy of Sciences, Moscow, 119334 Russia.

The low knock-in efficiency, especially in primary human cells, limits the use of the genome editing technology for therapeutic purposes, rendering it important to develop approaches for increasing the knock-in levels. In this work, the efficiencies of several approaches were studied using a model of knock-in of a construct coding for the peptide HIV fusion inhibitor MT-C34 into the human CXCR4 locus in the CEM/R5 T cell line. First, donor DNA modification was evaluated as a means to improve the efficiency of plasmid transport into the nucleus.

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Executive functions (EF) affect child competencies across domains in early childhood. Thus, this study examined: 1) the EF differences between young children with global developmental delays (GDD) and those with typical development (TD); 2) the relationship between mastery motivation (MM) and EF; 3) the association between developmental abilities and EF in both groups. Participants included 26 mother-child dyads of preschoolers with GDD (age 24-55 months) and 26 sex- and mental-age-matched dyads of TD preschoolers (age 15-29 months).

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