Biodepot Launcher: an app to install, manage and launch bioinformatics workflows.

We present the Biodepot Launcher, a desktop application that facilitates installation, management and deployment of bioinformatics workflows using the Biodepot-workflow-builder (Bwb). With the new app, Bwb can be started by double-clicking on an icon, eliminating the need for typing cryptic start up commands into the terminal. This creates an end-to-end graphical and easy-to-use interface to manage and launch containerized workflows on the local computer or cloud instances.

Investigating Risk Factors for Racial Disparity in E-Cigarette Use with PATH Study.

Background: Previous research has identified differences in e-cigarette use and socioeconomic factors between different racial groups However, there is little research examining specific risk factors contributing to the racial differences.

Objective: This study sought to identify racial disparities in e-cigarette use and to determine risk factors that help explain these differences.

Methods: We used Wave 5 (2018-2019) of the Adult Population Assessment of Tobacco and Health (PATH) Study.

Opportunities for Human-AI Collaboration in Remote Sighted Assistance.

Remote sighted assistance (RSA) has emerged as a conversational assistive technology for people with visual impairments (VI), where remote sighted agents provide realtime navigational assistance to users with visual impairments via video-chat-like communication. In this paper, we conducted a literature review and interviewed 12 RSA users to comprehensively understand technical and navigational challenges in RSA for both the agents and users. Technical challenges are organized into four categories: agents' difficulties in orienting and localizing the users; acquiring the users' surroundings and detecting obstacles; delivering information and understanding user-specific situations; and coping with a poor network connection.

Integrated SegFlow, µSIA, and UPLC for Online Sialic Acid Quantitation of Glycoproteins Directly from Bioreactors.

This study emphasizes the critical importance of closely monitoring and controlling the sialic acid content in therapeutic glycoproteins, including EPO, interferon-γ, Orencia, Enbrel, and others, as the level of sialylation directly impacts their pharmacokinetics (PK), immunogenicity, potency, and overall clinical performance due to its influence on protein clearance via hepatic asialoglycoprotein receptors (ASGPR). The ASGPR recognizes and binds to glycoproteins exposed to terminal galactose or N-acetylgalactosamine residues, leading to receptor-mediated endocytosis. Recent studies have demonstrated that sialylation of O-linked glycan plays a role in protecting against macrophage galactose lectin (MGL)-mediated clearance.

Enhancing engagement in parenting programs: A comparative study of in-person, online, and telehealth formats.

Introduction: Parenting programs are widely used to prevent and ameliorate children's emotional and behavioral problems but low levels of engagement undermine intervention effectiveness and reach within and beyond research settings. Technology can provide flexible and cost-effective alternate service-delivery formats for parenting programs, and studies are needed to assess the extent to which parents are willing to engage with digitally assisted formats.

Methods: After Deployment, Adaptive Parenting Tools (ADAPT) is an evidence-based parenting program for military families.

Patient "No-Show" Prior to Elective Primary Total Hip Arthroplasty Increases Risk of Postoperative Anemia.

Background: Patients who "no-show" (NS) clinical appointments are at a higher risk of poor healthcare outcomes. The objective of this study was to evaluate and characterize the relationship between patient NS prior to primary total hip arthroplasty (THA) and 90-day complication risk after THA.

Methods: We retrospectively reviewed 4147 patients undergoing primary THA.

Massive Periacetabular Osteolysis Treated With Acetabular Cup Retention and Cemented Screw Fixation.

Management of periacetabular osteolysis is a challenging dilemma in revision total hip arthroplasty. When the acetabular shell is well-fixed, the surgeon may prefer to retain the cup to minimize further bone loss. However, filling the surrounding defect can be difficult if the area of involvement is massive.

Single-Institution Experience With Nononcologic Total Femoral Replacement.

Background: Revision hip and knee arthroplasty volume continues to rise, and total femur replacement (TFR) remains a key salvage option in patients with extensive bone loss. Prior research has demonstrated mixed results of this procedure, and this study aimed to characterize the outcomes of nononcologic TFR in one of the largest single-center modern series.

Methods: A retrospective analysis was conducted on 23 nononcologic TFR procedures performed on 22 patients between 2012 and 2021.

Post-burns persistent inflammation leads to kidney PANoptosis with Caspases pathway activation.

Background: There is higher prevalence of chronic kidney disease (CKD) in burn patients after hospital discharge; however, the cause remains unclear. This study aimed to investigate the lasting impacts of severe burns on the kidneys and to explore potential treatments.

Methods: The study examined the effects of burning on healthy mice and adenine-induced CKD mice.

High-risk clones harboring β-lactamases: 2024 update.

Carbapenem-resistant is defined by the World Health Organization as a "high priority" in developing new antimicrobials. Indeed, the emergence and spread of multidrug-resistant (MDR) or extensively drug-resistant (XDR) bacteria increase the morbidity and mortality risk of infected patients. Genomic variants of that display phenotypes of MDR/XDR have been defined as high-risk global clones.

Depressive symptoms and quality of life among women experiencing infertility: The moderating effect of perceived social support.

Background: Infertility constitutes a leading reproductive health problem with profound psychosocial outcomes, including elevated depressive symptoms that compromise quality of life (QoL). While the literature has suggested social support as a protective psychological mechanism, its role in depressive symptoms and QoL among women with infertility remains underexplored. This study aimed to examine the moderating effect of perceived social support on the relationship between depressive symptoms and QoL among South Korean women experiencing infertility.

Roles of endoplasmic reticulum stress and activating transcription factors in Alzheimer's disease and Parkinson's disease.

Endoplasmic reticulum (ER) is a crucial organelle associated with cellular homeostasis. Accumulation of improperly folded proteins results in ER stress, accompanied by the reaction involving triggering unfolded protein response (UPR). The UPR is mediated through ER membrane-associated sensors, such as protein kinase-like ER kinase (PERK), inositol-requiring transmembrane kinase/endoribonuclease 1α, and activating transcription factor 6 (ATF6).

BcWRKY25-BcWRKY33A-BcLRP1/BcCOW1 module promotes root development for improved salt tolerance in Bok choy.

Root development is a complex process involving phytohormones and transcription factors. Our previous research has demonstrated that is significantly expressed in Bok choy roots under salt stress, and heterologous expression of increases salt tolerance and promotes root development in transgenic . However, the precise molecular mechanisms by which BcWRKY33A governs root development remain elusive.

Crosstalk between Gut Microbiota and Cancer Immunotherapy: Present Investigations and Future Perspective.

Gut microbiota is crucial for protecting the homeostasis of immune locally and systemically, and its dysbiosis is essentially correlated to tumorigenesis, cancer progression, and refractoriness to cancer treatments, including the novel immunotherapy. Increasing evidence unravel the intricate role of gut microbiota in reshaping tumor microenvironment and affecting the efficacy and toxicities of immunotherapy, which shed more light on the future applications of gut microbiota in efficacious biomarker and combination treatment of immunotherapy. To better grasp the underlying crosstalk between gut microbiota and immunotherapy, more experimental and clinical trials are indispensable for the customized gut microbiota-based treatments in cancer patients undergoing immunotherapy.

Glycogen homeostasis and mitochondrial DNA expression require motor neuron to muscle TGF-β/Activin signaling in Drosophila.

Maintaining metabolic homeostasis requires coordinated nutrient utilization between intracellular organelles and across multiple organ systems. Many organs rely heavily on mitochondria to generate (ATP) from glucose, or stored glycogen. Proteins required for ATP generation are encoded in both nuclear and mitochondrial DNA (mtDNA).

Adenosine deficiency facilitates CA1 synaptic hyperexcitability in the presymptomatic phase of a knockin mouse model of Alzheimer disease.

The disease's trajectory of Alzheimer disease (AD) is associated with and negatively correlated to hippocampal hyperexcitability. Here, we show that during the asymptomatic stage in a knockin (KI) mouse model of Alzheimer disease (APP; APPKI), hippocampal hyperactivity occurs at the synaptic compartment, propagates to the soma, and is manifesting at low frequencies of stimulation. We show that this aberrant excitability is associated with a deficient adenosine tone, an inhibitory neuromodulator, driven by reduced levels of CD39/73 enzymes, responsible for the extracellular ATP-to-adenosine conversion.

Daily oscillation of the excitation/inhibition ratio is disrupted in two mouse models of autism.

Alterations to the excitation/inhibition (E/I) ratio are postulated to underlie behavioral phenotypes in autism spectrum disorder (ASD) patients and mouse models. However, in wild type mice the E/I ratio is not constant, but instead oscillates across the 24-h day. Therefore, we tested whether E/I regulation, rather than the overall E/I ratio, is disrupted in two ASD-related mouse lines: KO and BTBR, models of syndromic and idiopathic ASD, respectively.

Exploring the utility of snRNA-seq in profiling human bladder tissue: A comprehensive comparison with scRNA-seq.

Single cell sequencing technologies have revolutionized our understanding of biology by mapping cell diversity and gene expression in healthy and diseased tissues. While single-cell RNA sequencing (scRNA-seq) has been widely used, interest in single-nucleus RNA sequencing (snRNA-seq) is growing due to its benefits, including the ability to analyze archival tissues and capture rare cell types that are challenging to dissociate. However, comparative studies across tissues have yielded mixed results, with some reporting enhanced cell type retention using snRNA-seq while others finding cell type identification to be challenging in snRNA-seq data.

Assessment of upper extremity functional capacity in following osteosynthesis for upper limb fractures using a novel method for determining total reachable workspace.

Objective: This study aimed to assess reachable workspace (RWS) in patients post-osteosynthesis of shoulder, elbow, or wrist fractures and explore correlations with self-reported function and kinesiophobia.

Design: An observational case-control study compared patients with fractures to a control group, utilizing questionnaires and 3D kinematic data.

Participants: The sample included 66 individuals who had undergone osteosynthesis: 21 with shoulder fractures, 10 with elbow fractures, and 22 with wrist fractures.

Human iPSC Reprogramming Success: The Impact of Approaches and Source Materials.

Since their discovery, human induced pluripotent stem cells (hiPSCs) have been instrumental in biomedical research, particularly in the fields of disease modelling, drug screening and regenerative therapies. Their use has significantly increased over recent years driven by the ability of hiPSCs to provide differentiated cell models without requiring embryonic stem cells. Furthermore, the transition from integrating to non-integrating reprogramming methodologies has contributed to the increase in utilisation.

Oral switch antibiotic therapy in uncomplicated bloodstream infection.

Background: The role of oral switch antibiotic therapy in uncomplicated bloodstream infection (BSI) remains unclear. This retrospective cohort study examines the effectiveness of oral switch compared with standard intravenous antibiotic therapy in uncomplicated BSI.

Methods: Adults with first episodes of uncomplicated monomicrobial BSI were admitted to 10 Prisma Health hospitals in South Carolina from January 2021 to June 2023 were included.

Enhancing Patient Choice Through the Development of Physical Therapist Online Biographies.

Provider web profiles are becoming popular locations for patients to seek information before their visits. This industry insight assesses patients' utilization of a physical therapy clinic's website to determine the impact the addition of physical therapist biographies had on patient perceptions and physical therapist selection. Forty-six percent of first-time patients ( = 786) indicated visiting the clinic's website prior to their visit.

Impaired Visuospatial Processing in Cerebral Visual Impairment Revealed by Performance on a Conjunction Visual Search Task.

Cerebral visual impairment (CVI) is a brain-based visual disorder associated with injury and/or maldevelopment of central visual pathways. Visuospatial processing impairments are a cardinal feature of the complex clinical profile of individuals with CVI. Here, we assessed visuospatial processing abilities using a classic conjunction search task.

Incremental Cost-Effectiveness Ratios (ICERs) and Revised Metformin Cost-Effectiveness Conclusions in the Diabetes Prevention Program/ Diabetes Prevention Program Outcomes Study.

Based on previously published US Diabetes Prevention Program (DPP) cost-effectiveness analyses (CEAs), metformin continues to be promoted as "cost-effective." We reviewed a 10-year CEA to assess this. Treatment alternatives included placebo, branded metformin and individual lifestyle modification.

Assessment of oral health status among occupational chefs and pantry workers in Hyderabad city.

Background: The chefs and pantry workers environment plays a crucial role in the general and oral health. These workers have some unusual work timings and have a lot of stress and anxiety, and they also consume tobacco, alcohol, and smoke the cigarettes, which has a greater impact on oral health.

Material And Methods: A total of 86 kitchen workers (53 chefs and 33 pantry workers) of ten well-known restaurants in Hyderabad city were included.