Genomic, transcriptomic, and T cell receptor profiling in stratifying response to first-line chemoradiotherapy or radiotherapy for esophageal squamous cell carcinoma.

Introduction: Esophageal squamous cell carcinoma (ESCC) accounts for 80% of esophageal cancer (EC) worldwide. The molecular characteristics of locally advanced ESCC have been extensively studied.

Methods: In this study, we investigate the genomic and transcriptomic characteristics and try to provide the basic T-cell receptors (TCRs) dynamics and its genomic and transcriptome association during the radiochemotherapy of ESCC using multi-omics analysis.

Diagnostic and prognostic performance of urine ubiquitin carboxy-terminal hydrolase L1 across multiple acute brain injury types - A longitudinal prospective cohort study.

Introduction: Ubiquitin carboxy-terminal hydrolase L1 (UCH-L1) is recognized as a diagnostic and prognostic blood biomarker for traumatic brain injury (TBI). This study aimed to evaluate whether UCH-L1 concentrations measured in patients' urine post-injury could serve as a diagnostic or prognostic biomarker for outcomes in various types of acute brain injuries (ABI).

Material And Methods: This pilot study included 46 ABI patients: aneurysmal subarachnoid hemorrhage (n = 22), ischemic stroke (n = 16), and traumatic brain injury (n = 8), along with three healthy controls.

Favorable response to ketogenic diet therapy in a patient with -related epilepsy.

Dynein Cytoplasmic 1 Heavy chain 1 (-related disorders are a spectrum of conditions including neurodevelopmental disorders, congenital brain malformations, and neuromuscular diseases. These clinical features may co-occur, with four main disease entities including epilepsy with developmental epileptic encephalopathy such as infantile epileptic spasms syndrome (IESS) and Lennox-Gastaut syndrome (LGS), axonal Charcot-Marie-Tooth disease type 2O, spinal muscular atrophy with lower extremity-predominance (SMALED), and congenital cortical malformations. Epilepsy associated with this disorder often becomes drug-resistant and requires multiple medications and, in some cases, non-pharmacological treatments.

Triadic balance and network evolution in predictive models of signed networks.

This paper introduces a novel approach for identifying dynamic triadic transformation processes, applied to five networks: three undirected and two directed. Our method significantly enhances the prediction accuracy of network ties. While balance theory offers insights into evolving patterns of triadic structures, its effects on overall network dynamics remain underexplored.

Non-linear enhancement of ultrafast X-ray diffraction through transient resonances.

Diffraction-before-destruction imaging with ultrashort X-ray pulses can visualize non-equilibrium processes, such as chemical reactions, with sub-femtosecond precision in the native environment. Here, a nanospecimen diffracts a single X-ray flash before it disintegrates. The sample structure can be reconstructed from the coherent diffraction image (CDI).

Impact of hospital readmission site on outcomes in acute ischemic stroke patients undergoing mechanical thrombectomy: a nationwide analysis.

Background: Mechanical thrombectomy (MT) significantly improves outcomes in patients with acute ischemic stroke due to large vessel occlusion. There is limited understanding of how the site of hospital readmission after MT influences patient outcomes. Addressing this gap may be important for optimizing post-MT care and improving long-term outcomes for stroke patients.

Epidemiology and outcomes of patients with cardiac arrest in the emergency department of a lower middle-income country.

Background: Advanced cardiovascular life support (ACLS) for cardiac arrest is a cornerstone of emergency care and yet remains poorly studied in low- and middle-income countries. We characterised the clinical epidemiology and outcomes of cardiac arrest and ACLS in an ED in central Haiti, a lower middle-income country with a nascent emergency care system.

Methods: We conducted a prospective observational study of adult and paediatric patients who suffered cardiac arrest in an academic hospital ED in central Haiti from January 2019 to August 2020.

Hypusinated and unhypusinated isoforms of the translation factor eIF5A exert distinct effects in models of pancreas development and function.

Hypusination of eukaryotic translation initiation factor 5A (eIF5A) is essential for its role in translation elongation and termination. Although the function of hypusinated eIF5A (eIF5A) in cellular proliferation is well-characterized, the role of its unhypusinated form (eIF5A) remains unclear. We hypothesized that eIF5A exerts independent, negative effects on cellular replication and metabolism, distinct from the loss of eIF5A.

Transforming Physiology and Healthcare through Foundation Models.

Recent developments in artificial intelligence (AI) may significantly alter physiological research and healthcare delivery. Whereas AI applications in medicine have historically been trained for specific tasks, recent technological advances have produced models trained on more diverse datasets with much higher parameter counts. These new, "foundation" models raise the possibility that more flexible AI tools can be applied to a wider set of healthcare tasks than in the past.

Reliability of remote video ratings of the scale for assessment and rating of ataxia.

Introduction: The Scale for Assessment and Rating of Ataxia (SARA) is a widely used clinical rating scale in ataxia. Remote video assessments of SARA examinations are increasingly used to reduce variability through centralized ratings. Remote video assessments have a high agreement with in-person ratings, but the intra- and inter-rater reliability of remote video ratings has not been examined.

Composite spin probes with adjustable oxygen sensitivity for pulse electron paramagnetic resonance imaging.

Purpose: Solid crystalline spin probes, such as lithium phthalocyanine (LiPc) and lithium octa-n-butoxynaphthalocyanine (LiNc-BuO), allow repeated oxygen measurement using electron paramagnetic resonance (EPR). Due to their short relaxation times, their use for pulse EPR oxygen imaging is limited. In this study, we developed and tested a new class of solid composite spin probes that modified the relaxation rates R and R of LiPc or LiNc-BuO probes, which allowed pO measurements in the full dynamic (0-760 torr) range.

Correlates of mental health of people living with HIV co-infected with SARS-CoV-2: findings from the COVIDHIV study in France.

Background: The COVID-19 pandemic has severely affected vulnerable populations, especially individuals living with HIV/AIDS (PLWHA). The convergence of HIV/AIDS and COVID-19 presents unique challenges, exacerbating existing health concerns and magnifying the strain on individuals already grappling with compromised immune systems. This study aimed to investigate the mental well-being repercussions faced by PLWHA co-infected with SARS-CoV-2 in France.

The Complete Genome Sequences of 22 Species of Owls (Strigiformes, Aves).

We present the complete genome sequences of 22 species of owls. Illumina sequencing was performed on DNA extracted from wild-caught specimens. The reads were assembled using a method followed by a finishing step.

High-throughput protein binder discovery by rapid in vivo selection.

Proteins that selectively bind to a target of interest are foundational components of research pipelines, diagnostics, and therapeutics. Current immunization-based, display-based, and computational approaches for discovering binders are laborious and time-consuming - taking months or more, suffer from high false positives - necessitating extensive secondary screening, and have a high failure rate, especially for disordered proteins and other challenging target classes. Here we establish Phage-Assisted Non-Continuous Selection of Protein Binders (PANCS-binders), an selection platform that links the life cycle of M13 phage to target protein binding though customized proximity-dependent split RNA polymerase biosensors, allowing for complete and comprehensive high-throughput screening of billion-plus member protein variant libraries with high signal-to-noise.

Risk of Severe Hypoglycemia After Initiation of Noninsulin Glucose-Lowering Therapies in Adults With Type 2 Diabetes at Moderate Cardiovascular Disease Risk.

In this emulated comparative effectiveness target trial of glucagon-like peptide 1 (GLP-1) receptor agonist, sodium-glucose cotransporter 2 (SGLT2) inhibitor, dipeptidyl peptidase 4 (DPP-4) inhibitor, and sulfonylurea therapy among adults with type 2 diabetes at moderate cardiovascular disease risk, sulfonylurea use was associated with a significantly higher risk of hypoglycemia requiring emergency department or hospital care than treatment with DPP-4 inhibitors, GLP-1 receptor agonists, or SGLT2 inhibitors. This consideration can guide the choice of glucose-lowering therapy in this highly prevalent patient population, in whom avoidance of hypoglycemia is important, yet among whom the risk of severe hypoglycemia has not been examined previously.

Circulating molecules reflect imaging biomarkers of hemorrhage in cerebral cavernous malformations.

Increases in mean lesional iron content by quantitative susceptibility mapping (QSM) by ≥6% and/or vascular permeability by dynamic contrast enhanced quantitative perfusion (DCEQP) by ≥40% on MRI have been associated with new symptomatic hemorrhage (SH) in cerebral cavernous malformations (CCMs). It is not known if plasma biomarkers can reflect these changes within the lesion proper. This cohort study enrolled 46 CCM patients with SH in the prior year.

Shear thickening inside elastic open-cell foams under dynamic compression.

We measure the response of open-cell polyurethane foams filled with a dense suspension of fumed silica particles in polyethylene glycol at compression speeds spanning several orders of magnitude. The gradual compressive stress increase of the composite material indicates the existence of shear rate gradients in the interstitial suspension caused by wide distributions in pore sizes in the disordered foam network. The energy dissipated during compression scales with an effective internal shear rate, allowing for the collapse of three data sets for different pore-size foams.

Characterization and Targeting of the Main Drivers of Elective Imaging Utilization: A Cross-sectional Study Applying Conjoint Analysis.

Objective: To characterize patient and referring physician preferences when selecting where to have elective imaging performed METHODS: In this Institutional Research Board approved survey-based study, online surveys were completed by 393 patients who had recently had a non-contrast MRI of the shoulder, lumbar spine, or knee within the past 2 years and 168 physicians who had ordered at least 12 such MRIs in the past year. The survey data included explicit questions about preferences as well as a set of conjoint choice screens. Conjoint analysis utilized a logistic model estimated using maximum likelihood estimation.

Cognitive Domains in the National Social Life, Health, and Aging Project.

Objectives: Cognition consists of specific domains that are differentially linked to health outcomes. We provide guidance on how to derive cognitive domains in the National Social Life, Health, and Aging Project (NSHAP) study. We suggest the use of a bifactor analysis to derive cognitive domains.

Electro-driven direct lithium extraction from geothermal brines to generate battery-grade lithium hydroxide.

As Li-ion batteries are increasingly being deployed in electric vehicles and grid-level energy storage, the demand for Li is growing rapidly. Extracting lithium from alternative aqueous sources such as geothermal brines plays an important role in meeting this demand. Electrochemical intercalation emerges as a promising Li extraction technology due to its ability to offer high selectivity for Li and its avoidance of harsh chemical regenerants.

Structural studies of the IFNλ4 receptor complex using cryoEM enabled by protein engineering.

IFNλ4 has posed a conundrum in human immunology since its discovery in 2013, with its expression linked to complications with viral clearance. While genetic and cellular studies revealed the detrimental effects of IFNλ4 expression, extensive structural and functional characterization has been limited by the inability to express and purify the protein, complicating explanations of its paradoxical behavior. In this work, we report a method for robust production of IFNλ4.

Individualized transfusion decisions to minimize adverse cardiovascular outcomes in patients with acute myocardial infarction and anemia.

Background: Risk-benefit tradeoffs between restrictive versus liberal red blood cell transfusion strategies may vary across individuals. This exploratory analysis aimed to derive and evaluate individualized treatment effects of defined transfusion strategies in patients with acute MI and anemia with the goal of minimizing adverse cardiovascular outcomes.

Methods: This study analyzed 3,447 (98.