Polymethoxyflavones from Gardenia oudiepe and semi-synthetic derivatives reduce nociception in mice: Evidence for the involvement of the MAPK pathway.

This study explores the potential of polymethoxyflavones (PMFs) and polyacetylated flavones (PAFs) as novel analgesic and anti-inflammatory agents. Eight derivatives, isolated from Gardenia oudiepe bud exudate or semi-synthesized from commercial kaempferol, underwent evaluations in various in vivo, in vitro, and in silico models. Acetic acid-, formalin-induced pain, and hot-plate tests were conducted in mice (n = 6).

Bioaccumulation, organotropism and fate of cadmium in Gammarus fossarum exposed through dietary pathway.

Despite a good knowledge of cadmium accumulation in Gammarus fossarum, studies to date have focused on Cd accumulated via the dissolved pathway, leaving aside the trophic pathway. The aim of this study was to assess cadmium organotropism and bioaccumulation processes following a trophic exposure of the species Gammarus fossarum. Adult male gammarids were fed with Cd contaminated alder leaves discs for 6 days and then with clean alder leaves for 12 days.

In Vitro Antiproliferative Activity of Echinulin Derivatives from Endolichenic Fungus sp. against Colorectal Cancer.

The endolichenic fungus sp. was isolated from the lichen harvested in France. sp.

The response of sea turtles to vocalizations opens new perspectives to reduce their bycatch.

Incidental capture of non-target species poses a pervasive threat to many marine species, with sometimes devastating consequences for both fisheries and conservation efforts. Because of the well-known importance of vocalizations in cetaceans, acoustic deterrents have been extensively used for these species. In contrast, acoustic communication for sea turtles has been considered negligible, and this question has been largely unexplored.

Shark critical life stage vulnerability to monthly temperature variations under climate change.

In a 10-month experimental study, we assessed the combined impact of warming and acidification on critical life stages of small-spotted catshark (Scyliorhinus canicula). Using recently developed frameworks, we disentangled individual and group responses to two climate scenarios projected for 2100 (SSP2-4.5: Middle of the road and SSP5-8.

Climate change by any other name: Social representations and language practices of coastal inhabitants on Mayotte Island in the Indian Ocean.

As population-related climate change research increases, so does the need to nuance approaches to this complex phenomenon, including issues related to cultural and linguistic translations. To explore how climate change is understood in understudied societies, a case-study approach is taken to address social representations of climate change by inhabitants of a Maore village in the French island of Mayotte. The study explores how local fishers understand the issue when considering observed environmental changes.

A proof-of-concept study on the versatility of liquid chromatography coupled to high-resolution mass spectrometry to screen for various contaminants and highlight markers of floral and geographical origin for different honeys.

The high-resolution mass spectrometry is a powerful analytical tool for improving food safety and authenticity, but still underused in official control laboratories. The present work is a proof-of-concept study overviewing how liquid-chromatography coupled to high-resolution mass spectrometry could be used simultaneously for large-scale screening of contaminants and differentiation of honey samples. Within this study, the samples were extracted using all-in-one QuEChERS-based protocol that allowed for analysis of various anthropogenic contaminants and endogenous compounds.

Isotopic and biochemical trophic markers reveal the complexity of interactions at the base of pelagic food webs (Mediterranean sea).

To gain insight into the impact of bottom-up changes in the plankton community on planktivorous fish in the context of the decline of small pelagic fisheries in the Northwestern Mediterranean Sea, we have conducted an extensive year-long study. The investigation combined biochemical analyses (proteins, carbohydrates, and lipids) with C and N stable isotope analyses (SIA) to simultaneously study phytoplankton, zooplankton, and eight planktivorous fish species (Engraulis encrasicolus, Sardina pilchardus, Sardinella aurita, Sprattus sprattus, Cepola macrophthalma, Chromis chromis, Boops boops, and Spicara maena). This study is the first to analyze both stable isotope and biochemical compositions in coastal particulate organic matter (POM) size classes (0.

Avoidance behaviour and toxicological impact of sunscreens in the teleost Chelon auratus.

There is increasing evidence that sunscreen, more specifically the organic ultra-violet filters (O-UVFs), are toxic for aquatic organisms. In the present study, we simulated an environmental sunscreen exposure on the teleost fish, Chelon auratus. The first objective was to assess their spatial avoidance of environmental concentrations of sunscreen products (i.

Wide-scope screening of multi-class contaminants in seafood using a novel sample preparation (QuEChUP) procedure coupled with UHPLC-Q-TOF-MS: Application for semi-quantitation of real seafood samples.

A high-resolution mass spectrometry screening method was developed and validated based on EU SANTE/11312/2021 guidelines for the analysis of 850 multi-class contaminants in commercial seafood samples. Samples were extracted using a novel sequential QuEChUP preparation method that combines the QuEChERS and QuPPe procedures. The screening detection limits (SDLs) and limits of identification (LOIs) were equal to or lower than 0.

Optimisation and implementation of QuEChERS-based sample preparation for identification and semi-quantification of 694 targeted contaminants in honey, jam, jelly, and syrup by UHPLC-Q/ToF high-resolution mass spectrometry.

A screening and semi-quantitative method was developed for the analysis of 694 various contaminants in honey, jam, jelly and syrup samples by ultrahigh-performance liquid chromatography and quadrupole time-of-flight mass spectrometry. Sample preparation, which was optimised using split factorial design, was based on acetate-buffered version of QuEChERS, followed by a clean-up step and a concentration step to enhance sensitivity of analytes. The method was validated according to SANTE/11312/2021 guidelines.

Approaches to determine pesticides in marine bivalves.

Due to agricultural runoff, pesticides end up in aquatic ecosystems and some accumulate in marine bivalves. As filter feeders, bivalves can accumulate high concentrations of chemicals in their tissue representing a potential risk to the health of human and aquatic ecosystems. So far, most of the studies dealing with pesticide contamination in marine bivalves, for example, in the French Atlantic and English Channel coasts, have focused on the old generation of pesticides.

Influence of the exposure concentration of dissolved cadmium on its organotropism, toxicokinetic and fate in Gammarus fossarum.

Information on the relationship between the exposure concentrations of metals and their biodistribution among organs remained scarce in invertebrates. The objective of this study was to investigate the effects of Cd concentration on the organotropism, toxico-kinetic and fate of this metal in different organs of gammarids exposed to dissolved radioisotope Cd. Gammarids male were exposed for 7 days to three environmental Cd concentrations (i.

First quantitative biomonitoring study of two ports (marina, commerce) in French littoral area: Evaluation of metals released into the marine environment and resulting from galvanic anodes.

Coastal areas are important interface environments between marine and terrestrial ecosystems and are also areas of high economic activity related, among others, to trade, fishing and tourism. The port areas of La Rochelle (France) are part of these areas with strong anthropic activities and are therefore subject to potential contamination, particularly with trace metals. Among the various sources of discharges, galvanic (or sacrificial) cathodic protection can be questioned.

Trace elements and δN values in micronekton of the south-western Indian Ocean.

Trace elements and δN values were analysed in micronekton (crustaceans, fishes and squids) sampled in the south-western Indian Ocean. Myctophids were associated with high concentrations of arsenic at La Pérouse and MAD-Ridge seamounts, and with lead and manganese at MAD-Ridge and in the Mozambique Channel. The difference in cadmium, copper and zinc concentrations between micronekton broad categories reflected differing metabolic and storage processes.

First study on the determination of baseline biomarkers in Mimachlamys varia for an intra-port environmental biomonitoring in French Atlantic coastline (La Rochelle).

The characterization of organic and inorganic environmental pollution in coastal ecosystems, such as port areas, is complex and difficult to carry out due to the effect of environmental variables, as well as anthropic activities. For this study, the objective was to define a statistical method, taking into account the confounding factors influence, to define reference values for biomarkers in the black scallop (Mimachlamys varia). Thus, for five biomarkers (SOD (Superoxyde Dismutase), GST (Glutathion-S Transferase), MDA (Malondialdehyde), AChE (Acetylcholinesterase) and LAC (Laccase)), reference data could be described for individuals placed on sites more or less strongly impacted by specific environmental contaminations in port areas for more than two years, which had never been done before.

Improving the monitoring of multi-class pesticides in baby foods using QuEChERS-UHPLC-Q-TOF with automated identification based on MS/MS similarity algorithms.

A screening method was developed for the multi-residue analysis of pesticides in baby foods using QuEChERS and UHPLC-Q-TOF. For sample preparation, the two-buffered versions of QuEChERS and different purification procedures were studied. False negatives and false positives were determined using different thresholds mentioned in the literature on the retention time and accurate mass measurement detection criteria.

Organ-specific accumulation of cadmium and zinc in Gammarus fossarum exposed to environmentally relevant metal concentrations.

One of the best approaches for improving the assessment of metal toxicity in aquatic organisms is to study their organotropism (i.e., the distribution of metals among organs) through a dynamical approach (i.

Development and validation according to the SANTE guidelines of a QuEChERS-UHPLC-QTOF-MS method for the screening of 204 pesticides in bivalves.

A qualitative screening high resolution mass spectrometry method was developed and validated according to the EU SANTE/12682/2019 guidelines for the analysis of 204 pesticides in seven commercial bivalve species spiked at three concentrations (0.01, 0.05 and, 0.

Intergenerational effects of environmentally-aged microplastics on the Crassostrea gigas.

This study focused on the impacts of aged aquaculture microplastics (MPs) on oysters (Crassostrea gigas). Adult oysters were exposed for two months to a cocktail of MPs representative of the contamination of the Pertuis Charentais area (Bay of Biscay, France) and issuing from oyster framing material. The MPs mixture included 28% of polyethylene, 40% of polypropylene and 32% of PVC (polyvinyl chloride).

Evidence of deleterious effects of microplastics from aquaculture materials on pediveliger larva settlement and oyster spat growth of Pacific oyster, Crassostrea gigas.

Plastic is currently used in aquaculture as a material for settlement and magnification of oyster spats. Plastic weathering and fragmentation under natural conditions can lead to the production of micro and nanoparticles and additive leakage, with potential toxic effects on marine life. This study investigates the effects of the exposure to microplastic (MPs) cocktail derived from aged aquaculture material on oyster pediveliger larvae (Crassostrea gigas).

Subchronic exposure to high-density polyethylene microplastics alone or in combination with chlortoluron significantly affected valve activity and daily growth of the Pacific oyster, Crassostrea gigas.

Nowadays, pesticides and microplastics (MPs) are commonly found in coastal waters worldwide. Due to their widespread use, their persistence and toxicity, they may induce adverse effects on physiology and behaviour of marine organisms such as the Pacific oyster (Crassostrea gigas). This study explored the growth and valve activity of juvenile oysters exposed for 24 days to two frequently detected pollutants in the Pertuis Charentais (South West, France): a herbicide (chlortoluron, 85 µg.