DYF-5 regulates intraflagellar transport by affecting train turnaround.

Intraflagellar transport (IFT) coordinates the transport of cargo in cilia and is essential for ciliary function. CILK1 has been identified as a key regulator of IFT. The mechanism by which it acts has, however, remained unclear.

Occupational Therapy Pre-Operative Intervention for Total Hip Replacement Patients; A Qualitative Enquiry.

This project aimed to explore the experiences of occupational therapists working in a specialist orthopedic hospital. Semi-structured interviews were carried out with eight occupational therapists working with total hip replacement patients, in an orthopedic hospital within the United Kingdom National Health Service (NHS) as part of a service review project. The data was recorded and transcribed and analyzed using the Reflexive Thematic Analysis.

Long-term safety and effectiveness of fenfluramine in children and adults with Dravet syndrome.

Objective: We analyzed the long-term safety and effectiveness of fenfluramine (FFA) in patients with Dravet syndrome (DS) in an open-label extension (OLE) study after participating in randomized controlled trials (RCTs) or commencing FFA de novo as adults.

Methods: Patients with DS who participated in one of three RCTs or were 19 to 35 years of age and started FFA de novo were included. Key endpoints were: incidence of treatment-emergent adverse events (TEAEs) in the safety population, and median percentage change in monthly convulsive seizure frequency (MCSF) from the RCT baseline to end of study (EOS) in the modified intent-to-treat (mITT) population.

Demystifying Meckel's diverticulum - a guide for the gastroenterologist.

Purpose Of Review: Meckel's diverticulum (MD) is a common congenital ileal diverticulum. Whilst mostly asymptomatic, 4-9% develop complications, such as small bowel obstruction, diverticulitis or bleeding. In 1933, Charles Mayo wrote that MD is 'frequently suspected, often looked for and seldom found', and it continues to pose a diagnostic challenge today.

Effects of different feeding strategies on egg production, eggshell quality, tibial mass and intestinal calcium transporter expression in laying hens.

1. This study examined feeding practices that could affect the expression of intestinal calcium transporter gene, tibial mass, eggshell quality and production performance in 25-week-old Hy-Line Brown Laying Hens.2.

Fan therapy for breathlessness - how do you do it and why?

Purpose Of The Review: This review summarises high-level evidence for fan therapy and adds a commentary on the relatively-neglected question of how to optimise benefits based on qualitative evidence, clinical experience and broader research and theory.

Recent Findings: Recent high-level evidence suggests the fan reduces time to recovery from episodic breathlessness rather than reduces daily levels over a longer period. Lower grade evidence suggests the fan can also help people increase their physical activity.

Evaluation of the European Organisation for Research and Treatment of Cancer Quality of Life Questionnaire Core 15 Palliative Care and the Functional Assessment of Chronic Illness Therapy-Palliative in assessing the quality of life in patients with advanced cancer.

Purpose Of Review: Two widely validated health-related quality of life (HR-QoL) tools, specifically designed for patients with advanced cancer, are the European Organisation for Research and Treatment of Cancer (EORTC) Quality of Life Questionnaire Core 15 Palliative Care (EORTC QLQ-C15-PAL) and the Functional Assessment of Chronic Illness Therapy-Palliative (FACIT-Pal-14). This systematic review aims to evaluate the use of EORTC QLQ-C15-PAL and FACIT-Pal-14 in prospective studies in patients with advanced cancer, focusing on study types, clinical settings, additional HR-QoL tools used, and completion rates.

Recent Findings: Sixty studies were included in the analysis.

Circulating Blood-Based Proteins in Psychopathology and Cognition: A Mendelian Randomization Study.

Importance: Peripheral (blood-based) biomarkers for psychiatric illness could benefit diagnosis and treatment, but research to date has typically been low throughput, and traditional case-control studies are subject to potential confounds of treatment and other exposures. Large-scale 2-sample mendelian randomization (MR) can examine the potentially causal impact of circulating proteins on neuropsychiatric phenotypes without these confounds.

Objective: To identify circulating proteins associated with risk for schizophrenia (SCZ), bipolar disorder (BD), and major depressive disorder (MDD) as well as cognitive task performance (CTP).

Retinal Microvascular Biomarker Assessment With Automated Algorithm and Semiautomated Software in the Montrachet Dataset.

Purpose: To compare automated and semiautomated methods for the measurement of retinal microvascular biomarkers: the automated retinal vascular morphology (AutoMorph) algorithm and the Singapore "I" Vessel Assessment (SIVA) software.

Methods: Analysis of retinal fundus photographs centered on optic discs from the population-based Montrachet Study of adults aged 75 years and older. Comparison and agreement evaluation with intraclass correlation coefficients (ICCs) between SIVA and AutoMorph measures of the central retinal venular and arteriolar equivalent, arteriolar-venular ratio, and fractal dimension.

Higher life's essential 8 score is associated with reduced risk of related surgery and all-cause mortality among 3,232 individuals with inflammatory bowel disease in a longitudinal cohort study.

Background: Previous studies indicated discrete lifestyle health metrics that benefit cardiovascular health are associated with better prognosis in inflammatory bowel disease (IBD), while evidence regarding the comprehensive impact of cardiovascular health remains limited. This study aimed to investigate the association between the overall cardiovascular health indicator, Life's Essential 8 (LE8), and the adverse outcomes of IBD.

Materials And Methods: We included 3,232 IBD participants recruited from the UK Biobank between 2006 and 2010.

Porous Silicon Nanoneedles Efficiently Deliver Adenine Base Editor to Correct a Recurrent Pathogenic COL7A1 Variant in Recessive Dystrophic Epidermolysis Bullosa.

Base editing, a CRISPR-based genome editing technology, enables precise correction of single-nucleotide variants, promising resolutive treatment for monogenic genetic disorders like recessive dystrophic epidermolysis bullosa (RDEB). However, the application of base editors in cell manufacturing is hindered by inconsistent efficiency and high costs, contributed by suboptimal delivery methods. Nanoneedles have emerged as an effective delivery approach, enabling highly efficient, non-perturbing gene therapies both in vitro and in vivo.

Tuning higher order structure in colloidal fluids.

Colloidal particles self assemble into a wide range of structures under external AC electric fields due to induced dipolar interactions [Yethiraj and Van Blaaderen, , 2003, , 513]. As a result of these dipolar interactions, at low volume fraction the system is modulated between a hard-sphere like state (in the case of zero applied field) and a "string fluid" upon application of the field. Using both particle-resolved experiments and computer simulations, we investigate the emergence of the string fluid with a variety of structural measures including two-body and higher-order correlations.

Sustainable 4D Printed Meta Biocomposite Materials for Programmable Structural Shape Changing.

Biological structures provide inspiration for developing advanced materials from sustainable resources, enabling passive structural morphing. Despite an increasing interest for parsimony-oriented innovation, sustainable shape-changing materials based on renewable resources remain underexplored. In this work, the architecture of a single plant fiber cell wall (S, for instance) is simplified to design novel concepts of 4D printed tubular moisture-driven structural actuators, using the hygromorphic properties of continuous flax fiber (cFF) reinforced materials.

Preserving the Biologically Coherent Generic Concept of , 'Plant Destroyer'.

is a long-established, well known and globally important genus of plant pathogens. Phylogenetic evidence has shown that the biologically distinct, obligate biotrophic downy mildews evolved from at least twice. Since, cladistically, this renders 'paraphyletic', it has been proposed that evolutionary clades be split into multiple genera (Runge et al.

The Use of Customized 3D-Printed Guides in Anterior Cruciate Ligament Reconstruction Compared With Conventional Techniques: A Systematic Review and Meta-analysis.

Background: Accurate femoral tunnel positioning is essential for successful anterior cruciate ligament (ACL) reconstruction. Tunnel malposition can happen due to limited arthroscopic visibility as well as anatomic variance. The use of customized patient-specific guides can optimize surgical planning and enhance accuracy.

The PP2A-B56 Binding Site LxxIxE Contributes to Asp-Mediated Spindle Pole Stability.

The organization of microtubules into a mitotic spindle is critical for animal cell proliferation and involves the cooperation of hundreds of proteins whose molecular roles and regulation are not fully understood. The protein product of the Drosophila gene abnormal spindle, Asp, is a microtubule-associated protein required for correct mitotic spindle formation. To better understand the contribution of Asp to microtubule organization during spindle formation, we reverse-engineered flies to express a version of Asp (Asp), predicted to have lost its ability to bind the phosphatase trimer PP2A-B56.

An In Situ Characterisation Method for 3-D Electrospun Foams.

Three-dimensional electrospun foams are emerging in a diversity of applications. However, their characterisation involves procedures to calculate fibre diameter and porosity, which take considerable time. Hence, in this paper, an in situ characterisation method is presented based on signal features of the grounding voltage.

Metaboloepigenetics: Role in the Regulation of Flow-Mediated Endothelial (Dys)Function and Atherosclerosis.

Endothelial dysfunction is the main initiating factor in atherosclerosis. Through mechanotransduction, shear stress regulates endothelial cell function in both homeostatic and diseased states. Accumulating evidence reveals that epigenetic changes play critical roles in the etiology of cardiovascular diseases, including atherosclerosis.

A Cross-Sectional Exploratory Study of Rat Sarcoid (Ras) Activation in Women with and Without Polycystic Ovary Syndrome.

: Rat sarcoma (Ras) proteins, Kirsten, Harvey, and Neuroblastoma rat sarcoma viral oncogene homolog (KRAS, HRAS, and NRAS, respectively), are a family of GTPases, which are key regulators of cellular growth, differentiation, and apoptosis through signal transduction pathways modulated by growth factors that have been recognized to be dysregulated in PCOS. This study explores Ras signaling proteins and growth factor-related proteins in polycystic ovary syndrome (PCOS). : In a well-validated PCOS database of 147 PCOS and 97 control women, plasma was batch analyzed using Somascan proteomic analysis for circulating KRas, Ras GTPase-activating protein-1 (RASA1), and 45 growth factor-related proteins.

Statins as an Adjunctive Antithrombotic Agent in Thrombotic Antiphospholipid Syndrome: Mechanisms and Clinical Implications.

The thrombotic physiopathology of antiphospholipid syndrome (APS) is complex, heterogeneous, and dynamic. While venous thromboembolism (VTE) is the most common initial presentation, arterial thrombotic events (ATE) become more frequent in advanced stages and are associated with high morbidity and mortality. Despite the use of oral anticoagulants (OACs), thrombotic APS remains associated with a high risk of recurrent thrombosis.

Trapped in the NETs: Multiple Roles of Platelets in the Vascular Complications Associated with Neutrophil Extracellular Traps.

Neutrophil extracellular traps (NETs) have received significant attention in recent years for their role in both the immune response and the vascular damage associated with inflammation. Platelets have been described as critical components of NETs since the initial description of this physio-pathological response of neutrophils. Platelets have been shown to play a dual role as responders and also as stimulators of NETs.