4 results match your criteria: "U.S. Geological Survey-Western Fisheries Research Center[Affiliation]"
Dis Aquat Organ
July 2022
U.S. Geological Survey - Western Fisheries Research Center, Marrowstone Marine Field Station, 616 Marrowstone Point Road, Nordland, WA 98358, USA.
In recent decades, evidence has accumulated to suggest that the widespread and highly variable parasite Ichthyophonus hoferi is actually a species complex. Highly plastic morphology and a general lack of defining structures has contributed to the likely underestimate of biodiversity within this group. Molecular methods are a logical next step in the description of these parasites, but markers used to date have been too conserved to resolve species boundaries.
View Article and Find Full Text PDFJ Vet Diagn Invest
July 2016
U.S. Geological Survey-Western Fisheries Research Center, Seattle, WA (Purcell, Pearman-Gillman, Thompson, Winton, Emmenegger)School of Aquatic and Fishery Sciences, University of Washington, Seattle, WA (Thompson)U.S. Geological Survey-Marrowstone Marine Field Station, Nordland, WA (Gregg, Hart, Hershberger).
Viral erythrocytic necrosis (VEN) is a disease of marine and anadromous fish that is caused by the erythrocytic necrosis virus (ENV), which was recently identified as a novel member of family Iridoviridae by next-generation sequencing. Phylogenetic analysis of the ENV DNA polymerase grouped ENV with other erythrocytic iridoviruses from snakes and lizards. In the present study, we identified the gene encoding the ENV major capsid protein (MCP) and developed a quantitative real-time PCR (qPCR) assay targeting this gene.
View Article and Find Full Text PDFInfectious diseases are economically detrimental to aquaculture, and with continued expansion and intensification of aquaculture, the importance of managing infectious diseases will likely increase in the future. Here, we use evolution of virulence theory, along with examples, to identify aquaculture practices that might lead to the evolution of increased pathogen virulence. We identify eight practices common in aquaculture that theory predicts may favor evolution toward higher pathogen virulence.
View Article and Find Full Text PDFJ Fish Dis
January 2011
U.S. Geological Survey-Western Fisheries Research Center, Marrowstone Marine Field Station, Nordland, WA 98358, USA.
Procedures for a viral replication in excised fin tissue (VREFT) assay were adapted to Pacific herring, Clupea pallasii, and optimized both to reduce processing time and to provide the greatest resolution between naïve herring and those previously exposed to viral haemorrhagic septicaemia virus (VHSV), Genogroup IVa. The optimized procedures included removal of the left pectoral fin from a euthanized fish, inoculation of the fin with >10(5) plaque-forming units (PFU) mL(-1) VHSV for 1 h, rinsing the fin in fresh medium six times to remove unadsorbed virions, incubation of the fin in fresh medium for 4 days and enumeration of the viral titre in a sample of the incubation medium by plaque assay. The optimized VREFT assay was effective at identifying the prior exposure history of laboratory-reared Pacific herring to VHSV.
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