Macrolactin A Is an Inhibitor of Protein Biosynthesis in Bacteria.

Macrolactin A (McA) is a secondary metabolite produced by Bacillus species. It has been known for its antimicrobial properties since the late 1980s, although the exact mechanism of its antibacterial activity remains unknown. In this study, we have found that McA is an inhibitor of protein synthesis in bacteria.

Designing and clinical application of a 3D-printed personalized model of a radiofrequency needle guide with a maxillary fixator for puncture of the gasserian ganglion for trigeminal neuralgia treatment.

Objective: The purpose of this study was to present a newly designed 3D-printed personalized model (3D PPM) of a radiofrequency needle guide with a maxillary fixation for gasserian ganglion (GG) puncture.

Methods: Implementation of 3D CT-guided radiofrequency therapy of the GG with and without use of 3D PPM was analyzed. The following parameters were assessed: radiation time, dose area product, air kerma reference point, pain severity during the puncture needle insertion, prosopalgia regression degree (according to visual analog scale) and the severity of facial numbness (according to the Barrow Neurological Institute scale) in the early postoperative period, and postpuncture complications.

Regional Coleoptera Fauna: Applying Different Methods to Study Species Diversity in a Single Region.

In order to study regional faunas, one or two methods of studying Coleoptera are often used. However, a comparison of several ways of studying shows that it is more expedient to use more than one of them. Six different methods of catching Coleoptera used in studies of the biodiversity of regional faunas were compared.

Higher vulnerability to poor circadian light hygiene in individuals with a history of COVID-19.

Seven-day actigraphy was performed within 1 month in 122 community-dwelling adults (mean age 24.40 y, 31 (25.4%) men) in the same city of Tyumen, Russia.

Flavuside B exhibits antioxidant and anti-inflammatory properties in Staphylococcus aureus infected skin wound and affect the expression of genes controlling bacterial quorum sensing.

Aims: The aim of this study was to evaluate the antioxidant and anti-inflammatory effects of marine fungal cerebroside flavuside B (FlaB) on Staphylococcus aureus-infected keratinocytes in in vitro skin wounds and to identify FlaB targets in bacterial and human cells.

Methods And Results: A combination of enzyme-linked immunosorbent assay (ELISA), plate spectrofluorimetry, and flow cytometry with fluorescence dye staining, scratch assay, and real-time cell imaging techniques was used to investigate the effects of FlaB on S. aureus-infected HaCaT keratinocytes.

The eusocial non-code: Unveiling the impact of noncoding RNAs on Hymenoptera eusocial evolution.

Eusociality, characterized by reproductive division of labor, cooperative brood care, and multi-generational cohabitation, represents a pinnacle of complex social evolution, most notably manifested within the Hymenoptera order including bees, ants, and wasps. The molecular underpinnings underlying these sophisticated social structures remain an enigma, with noncoding RNAs (ncRNAs) emerging as crucial regulatory players. This article delves into the roles of ncRNAs in exerting epigenetic control during the development and maintenance of Hymenopteran eusociality.

Circadian Disruption in Glaucoma: Causes, Consequences, and Countermeasures.

This review explores the intricate relationship between glaucoma and circadian rhythm disturbances. As a principal organ for photic signal reception and transduction, the eye plays a pivotal role in coordinating the body's circadian rhythms through specialized retinal ganglion cells (RGCs), particularly intrinsically photosensitive RGCs (ipRGCs). These cells are critical in transmitting light signals to the suprachiasmatic nucleus (SCN), the central circadian clock that synchronizes physiological processes to the 24-hour light-dark cycle.

[Difficulties in lifetime diagnosis of Creutzfeldt-Jakob disease].

Creutzfeldt-Jakob disease (CJD) is a classic representative of the group of prion diseases and is characterized by progressive degeneration of the structures of the nervous system with a variety of neurological symptoms, steady progression and inevitable death. The disease is based on a change in the tertiary structure of the protein, which leads to disruption of the normal functioning of cells. Despite the fact that the etiology and pathogenesis of CJD are now well studied, intravital diagnosis of this serious disease remains difficult due to the peculiarities of the pathological process (unusually long incubation period, variety of clinical symptoms), the lack of pathognomonic markers that make it possible to make a diagnosis with a high degree of confidence, and also insufficient awareness of medical workers.

RNA-Binding S1 Domain in Bacterial, Archaeal and Eukaryotic Proteins as One of the Evolutionary Markers of Symbiogenesis.

The RNA-binding S1 domain is a β-barrel with a highly conserved RNA-binding site on its surface. This domain is an important part of the structures of different bacterial, archaeal, and eukaryotic proteins. A distinctive feature of the S1 domain is multiple presences (structural repeats) in proteins and protein complexes.

Mutations Associated with Cushing's Disease Alter Protein Structure Dynamics.

The adenomas in Cushing's disease frequently exhibit mutations in exon 14, within a binding motif for the regulatory protein 14-3-3 located between the catalytic domain (DUB), responsible for ubiquitin hydrolysis, and the WW-like domain that mediates autoinhibition, resulting in constantly active USP8. The exact molecular mechanism of deubiquitinase activity disruption in Cushing's disease remains unclear. To address this, Sanger sequencing of was performed to identify mutations in corticotropinomas.

New Galumnidae (Acari, Oribatida) from the Merida and Zulia States, Venezuela.

Three new species of the family Galumnidae (Oribatida)-Sacculogalumna zuliaensis sp. nov., Pergalumna (Pergalumna) convergens sp.

New Ceratorchestes (Acari, Oribatida, Peloppiidae) from Venezuela.

Three new species of Ceratorchestes (Ceratorchestes) (Oribatida, Peloppiidae)-C. (C.) tripartitus sp.

Juvenile instars of Sellnickia caudata (Michael, 1908) (Acari, Oribatida, Sellnickiidae).

Juvenile instars of Sellnickia caudata (Michael, 1908) are described, based on specimens collected from tree branches and leaves of Dipterocarpus alatus in Vietnam.

Ontogenetic instars of the oribatid mite Eupterotegaeus nortoni sp. nov. (Acari, Oribatida, Cepheusidae) from U.S.A.

A new species of the genus Eupterotegaeus (Oribatida, Cepheusidae) is described from the U.S.A.

Description of juvenile instars of Scapheremaeus tonatiuh Palacios-Vargas, Ríos & Vázquez, 1998 (Acari, Oribatida, Cymbaeremaeidae).

The oribatid mite species Scapheremaeus tonatiuh (Oribatida, Cymbaeremaeidae) is recorded for the first time in the U.S.A.

Contribution to knowledge of the oribatid mite genus Oribatodes (Acari, Oribatida, Cepheusidae), with supplementary descriptions of two species and data on juvenile instars.

The supplementary descriptions of two species of Oribatodes (Oribatida, Cepheusidae), O. mirabilis Banks, 1895 and O. reticulatus (Sitnikova, 1979), are presented, based on adults collected from the U.

Ontogenetic instars of Basilobelba werneri Mahunka, 1982 (Acari, Oribatida, Basilobelbidae).

Basilobelba werneri (Oribatida, Basilobelbidae) was collected from an alpine cloud forest in Guatemala. This is the first representative of the family Basilobelbidae in the Guatemalan fauna. A supplementary description of adult B.

Impact of diabetes on epicardial reperfusion and mortality in a contemporary STEMI population undergoing mechanical reperfusion: Insights from the ISACS STEMI COVID 19 registry.

Background And Aim: Diabetes has been shown in last decades to be associated with a significantly higher mortality among patients with ST-segment elevation myocardial infarction (STEMI) treated with primary PCI (PPCI). Therefore, the aim of current study was to evaluate the impact of diabetes on times delays, reperfusion and mortality in a contemporary STEMI population undergoing PPCI, including treatment during the COVID pandemic.

Methods And Results: The ISACS-STEMI COVID-19 is a large-scale retrospective multicenter registry involving PPCI centers from Europe, Latin America, South-East Asia and North-Africa, including patients treated from 1st of March until June 30, 2019 and 2020.

Adeno-Associated Viral Vectors in the Treatment of Epilepsy.

Epilepsy is a brain disorder characterized by a persistent predisposition to epileptic seizures. With various etiologies of epilepsy, a significant proportion of patients develop pharmacoresistance to antiepileptic drugs, which necessitates the search for new therapeutic methods, in particular, using gene therapy. This review discusses the use of adeno-associated viral (AAV) vectors in gene therapy for epilepsy, emphasizing their advantages, such as high efficiency of neuronal tissue transduction and low immunogenicity/cytotoxicity.

ANALYSIS OF BLOOD PARAMETERS IN TYUMEN RESIDENTS WITH COVID-19 IN CATAMNESIS AND/OR VACCINATED AGAINST A NEW CORONAVIRUS INFECTION.

Currently, much is known about the causative agent of Covid-19. The situation with the spread of a new coronavirus infection is under control. Preventive measures against this disease have been developed and are being actively implemented.

[Biomechanical control of myopia: potential of bidirectional corneal applanation with high-speed video recording].

Unlabelled: The introduction of bidirectional corneal applanation technologies into clinical practice made possible the in vivo assessment of biomechanical properties of the fibrous tunic in myopic eyes.

Purpose: This study analyzes the potential of bidirectional corneal applanation with high-speed video recording for biomechanical control of myopia, focusing on the biomechanical parameters of the sclera and cornea.

Material And Methods: The study included 129 patients (168 eyes) with myopia ranging from -0.

[PET/CT MOLECULAR CELL IMAGING OF ACUTE PYELONEPHRITIS IN THE DIAGNOSIS OF FEVER OF UNKNOWN ORIGIN].

In addition to tumor imaging, advanced high-tech PET/CT imaging is increasingly used to elucidate the underlying causes of fever of unknown origin caused by various inflammatory processes, including pyelonephritis. Two clinical cases of whole-body PET/CT with 18F-FDG glucose, which allowed to visualize the molecular and cellular features of acute pyelonephritis as the cause of fever of unknown origin, are presented in the article. The scientifically based mechanisms of this process and the reliability of the results are discussed.