11 results match your criteria: "Turkey. budak@sabanciuniv.edu[Affiliation]"

Application of Tissue Culture and Transformation Techniques in Model Species Brachypodium distachyon.

Methods Mol Biol

June 2018

Faculty of Engineering and Natural Sciences, Molecular Biology, Genetics and Bioengineering Program, Sabanci University, Orhanli 34956 Tuzla, Istanbul, Turkey.

Brachypodium distachyon has recently emerged as a model plant species for the grass family (Poaceae) that includes major cereal crops and forage grasses. One of the important traits of a model species is its capacity to be transformed and ease of growing both in tissue culture and in greenhouse conditions. Hence, plant transformation technology is crucial for improvements in agricultural studies, both for the study of new genes and in the production of new transgenic plant species.

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Microfabricated tools for quantitative plant biology.

Analyst

March 2017

Department of Molecular Biology, Genetics and Bioengineering, Faculty of Engineering and Natural Sciences, Sabanci University, 34956, Istanbul, Turkey.

The development of microfabricated devices that will provide high-throughput quantitative data and high resolution in a fast, repeatable and reproducible manner is essential for plant biology research. Plants have been intensely explored since the beginning of humanity, especially for medical needs. However, plant biology research is still laborious, lacking the latest technological advancements in the laboratory practices.

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Wheat miRNA ancestors: evident by transcriptome analysis of A, B, and D genome donors.

Funct Integr Genomics

May 2017

Molecular Biology, Genetics and Bioengineering Program, Sabanci University, 34956, Istanbul, Turkey.

MicroRNAs are critical players of post-transcriptional gene regulation with profound effects on the fundamental processes of cellular life. Their identification and characterization, together with their targets, hold great significance in exploring and exploiting their roles on a functional context, providing valuable clues into the regulation of important biological processes, such as stress tolerance or environmental adaptation. Wheat is a hardy crop, extensively harvested in temperate regions, and is a major component of the human diet.

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The physical map of wheat chromosome 5DS revealed gene duplications and small rearrangements.

BMC Genomics

June 2015

Sabanci University Nanotechnology Research and Application Centre (SUNUM), Sabanci University, Universite Cad. Orta Mah. No: 27, Tuzla, 34956, Istanbul, Turkey.

Background: The substantially large bread wheat genome, organized into highly similar three sub-genomes, renders genomic research challenging. The construction of BAC-based physical maps of individual chromosomes reduces the complexity of this allohexaploid genome, enables elucidation of gene space and evolutionary relationships, provides tools for map-based cloning, and serves as a framework for reference sequencing efforts. In this study, we constructed the first comprehensive physical map of wheat chromosome arm 5DS, thereby exploring its gene space organization and evolution.

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Background: The ~17 Gb hexaploid bread wheat genome is a high priority and a major technical challenge for genomic studies. In particular, the D sub-genome is relatively lacking in genetic diversity, making it both difficult to map genetically, and a target for introgression of agriculturally useful traits. Elucidating its sequence and structure will therefore facilitate wheat breeding and crop improvement.

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Proteome changes in wild and modern wheat leaves upon drought stress by two-dimensional electrophoresis and nanoLC-ESI-MS/MS.

Plant Mol Biol

September 2013

Biological Sciences and Bioengineering Program, Faculty of Engineering and Natural Sciences, Sabancı University, Tuzla, Istanbul 34956, Turkey.

To elucidate differentially expressed proteins and to further understand post-translational modifications of transcripts, full leaf proteome profiles of two wild emmer (Triticum turgidum ssp. dicoccoides TR39477 and TTD22) and one modern durum wheat (Triticum turgidum ssp. durum cv.

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Dehydration stress-responsive miRNA in Brachypodium distachyon: evident by genome-wide screening of microRNAs expression.

OMICS

November 2011

Biological Sciences and Bioengineering Program, Faculty of Engineering and Natural Sciences, Sabanci University, Istanbul, Turkey.

There is a lack of knowledge on the tissue-specific expression of miRNAs in response to dehydration stress in Brachypodium (Brachypodium distachyon (L.) Beauv), a model for temperate grass species. In this study, miRNA expression patterns of drought-tolerant Brachypodium were investigated using the miRNA microarray platform.

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Revealing constitutively expressed resistance genes in Agrostis species using PCR-based motif-directed RNA fingerprinting.

Genet Res

December 2006

Biological Science and Bioengineering Program, Faculty of Engineering and Natural Sciences, Sabanci University, Orhanli 34956, Tuzla/Istanbul, Turkey.

Agrostis species are mainly used in athletic fields and golf courses. Their integrity is maintained by fungicides, which makes the development of disease-resistance varieties a high priority. However, there is a lack of knowledge about resistance (R) genes and their use for genetic improvement in Agrostis species.

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Molecular characterization of cDNA encoding resistance gene-like sequences in Buchloe dactyloides.

Mol Biotechnol

November 2006

Sabanci University, Biological Science and Bioengineering Program, Orhanli, 34956, Tuzla/Istanbul, Turkey.

Current knowledge of resistance (R) genes and their use for genetic improvement in buffalograss (Buchloe dactyloides [Nutt.] Engelm.) lag behind most crop plants.

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Understanding ploidy complex and geographic origin of the Buchloe dactyloides genome using cytoplasmic and nuclear marker systems.

Theor Appl Genet

November 2005

Faculty of Engineering and Natural Science, Biological Science and Bioengineering Program, Sabanci University, Orhanli, Tuzla 34956, Istanbul, Turkey.

Characterizing and inferring the buffalograss [Buchloe dactyloides (Nutt.) Engelm.] genome organization and its relationship to geographic distribution are among the purposes of the buffalograss breeding and genetics program.

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Evolution of Buchloë dactyloides based on cloning and sequencing of matK, rbcL, and cob genes from plastid and mitochondrial genomes.

Genome

June 2005

Sabanci University, Faculty of Engineering and Natural Science, Biological Science, and Bioengineering, Istanbul, Turkey.

Buffalograss (Buchloë dactyloides (Nutt.) Englem), a C4 turfgrass species, is native to the Great Plains region of North America. The evolutionary implications of buffalograss are unclear.

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