13 results match your criteria: "Tongli Medical College[Affiliation]"

Induced regeneration of articular cartilage - identification of a dormant regeneration program for a non-regenerative tissue.

Development

November 2023

Department of Veterinary Physiology and Pharmacology, School of Veterinary Medicine and Biomedical Sciences, Texas A&M University, College Station, TX 77843, USA.

A mouse organoid culture model was developed to regenerate articular cartilage by sequential treatment with BMP2 and BMP9 (or GDF2) that parallels induced joint regeneration at digit amputation wounds in vivo. BMP9-induced chondrogenesis was used to identify clonal cell lines for articular chondrocyte and hypertrophic chondrocyte progenitor cells from digit fibroblasts. A protocol that includes cell aggregation enhanced by BMP2 followed by BMP9-induced chondrogenesis resulted in the differentiation of organized layers of articular chondrocytes, similar to the organization of middle and deep zones of articular cartilage in situ, and retained a differentiated phenotype following transplantation.

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Classification and identification of molecules through factor analysis method based on terahertz spectroscopy.

Spectrochim Acta A Mol Biomol Spectrosc

June 2018

Department of Urology, Tongji Hospital, Tongli Medical College, Huazhong University of Science and Technology, Wuhan 430074, Hubei, China.

By means of factor analysis approach, a method of molecule classification is built based on the measured terahertz absorption spectra of the molecules. A data matrix can be obtained by sampling the absorption spectra at different frequency points. The data matrix is then decomposed into the product of two matrices: a weight matrix and a characteristic matrix.

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Objective: To observe the pathological changes of major organs in rats with acute Dysosma versipellis poisoning and investigate the toxic mechanism and the injuries of target tissues and organs.

Methods: Forty Sprague-Dawley (SD) rats were randomly divided into three experimental groups, which were given the gavage with 0.5, 1.

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Objective: To investigate the expression and significance of beta-receptor ( p-R) in infantile hemangioma.

Methods: The expression of beta-R was detected by immunohistochemistry in infantile hemangioma (40 cases), venous malformation (20 cases) and normal skin (10 cases).

Results: The positive expression rate of beta2-R was 70% (28/40) in infantile hemangioma, while no expression of beta2-R in venous malformation and normal skin.

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[The study of the characteristics and influencing factors of pneumoconiosis among workers exposed to dusts in an iron mine].

Zhonghua Lao Dong Wei Sheng Zhi Ye Bing Za Zhi

January 2012

MOE Key Lab of Environmental and Health, School of Public Health, Tongli Medical College, Huazhong University of Science & Technology, Wuhan 430030, China

Objective: To analyze the prevalence characteristics and influence factors of pneumoconiosis of workers exposed to dusts in an iron mine, to provide the base of preventive measures for pneumoconiosis of iron mine.

Methods: The subjects of cohort study were all workers exposed to dusts for at least one year registered in an iron mine during 1960 to 1974, and followed-up to the end in 2003. The cases with pneumoconiosis were diagnosed by the local diagnosis group of pneumoconiosis, according to the national diagnostic criteria of pneumoconiosis.

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[The effects of ammonium perchlorate on thyroid function and testis in male rats].

Zhonghua Lao Dong Wei Sheng Zhi Ye Bing Za Zhi

August 2011

Public Health School, Tongli Medical College, Huazhong University of Science and Technology, Wuhan, China.

Objective: To study the effects of ammonium perchlorate (AP) on the levels of thyroid hormone and the testis function of male rats.

Methods: Twenty male rats were randomly divided into 4 groups: control group, low, middle and high AP group. The rats were exposed orally to 0, 130, 260 and 520 mg AP/kg a day for 80 days.

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[Expression of heme oxygenase enzyme in the testis tissue and azoospermia].

Zhonghua Nan Ke Xue

August 2011

Research Institute of Family Planning/Center of Reproductive Medicine, Tongli Medical College, Huazhong University of Science and Technology, Wuhan, Hubei 430030, China.

Objective: To investigate the location of heme oxygenase (HO) enzyme in the human testis, and explore the correlation of the expression of HO enzyme with azoospermia by analyzing its different expression levels in the testes of nonobstructive azoospermia, obstructive azoospermia and normal men.

Methods: We detected the location of the cells expressing HO enzyme in the human testis tissue using immunohistochemistry, determined the mRNA and protein expression levels of HO-1 and HO-2 in the testes of azoospermia patients and normal healthy men by RT-fluorescence quantitative PCR (RT-FQ-PCR) and Western blot, and explored the correlation of HO expressions with the pathogenesis of azoospermia.

Results: HO-1 enzyme was expressed mainly in the Sertoli cells and HO-2 enzyme chiefly in the germ cells of the testis tissue.

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Objective: To evaluate the effects of red clover isoflavones on the proliferation and apoptosis of human benign prostatic hyperplasia (BPH) stromal cells.

Methods: We treated human prostate stromal cells with red clover isoflavones at the concentration of 12.5, 25, 50 and 100 microg/ml, and established a PBS blank control, a dimethyl sulphoxide (DMSO) negative control and four finasteride positive control groups (at the concentration of 12.

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[Value of cardiopulmonary bypass for locally advanced lung cancer involving the left atrium].

Zhonghua Zhong Liu Za Zhi

November 2006

Department of Cardiothoracic Surgery, Tongji Hospital, Tongli Medical College, Huazhong University of Sciences and Technology, Wuhan 430030, China.

Objective: To review the experience and assess the value of cardiopulmonary bypass (CPB) in the treatment of locally advanced lung cancer involving the left atrium.

Methods: From Jan. 1999 to Dec.

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Expression of survivin in human non-Hodgkin lymphoma and its correlation with proliferation and angiogenesis.

J Huazhong Univ Sci Technolog Med Sci

December 2013

Department of Pathology, School of Basic Medical Sciences, Tongli Medical College, Huazhong University of Science and Technology, Wuhan 430030, China.

In order to investigate the expression change of survivin in non-Hodgkin lymphoma (NHL) and its possible effects on NHL development, the expression of survivin, Ki-67, caspase3 and FVIIIRAg in reactive lymphoid hyperplasia (RH) and NHL was detected by immunohistochemical assay, and apoptosis index (AI) in RH and NHL by TUNEL analysis. The results showed that the expression of survivin is significantly higher in aggressive NHL than in indolent NHL (P<0.01), while there was no statistically significant difference between RH and indolent NHL (P>0.

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Construction of human ScFv phage display library against ovarian tumor.

J Huazhong Univ Sci Technolog Med Sci

December 2013

Department of Nuclear Medicine, Tongji Hospital, Tongli Medical College, Huazhong Universty of Science and Technology, Wuhan 430030, China.

In order to construct a single chain fragment variable (ScFv) phage display library against ovarian tumor, by using RT-PCR, the human heavy chain variable region genes (VH) and light chain variable region genes (VL) were amplified from lymphocytes of ovarian tumor patients and subsequently assembled into ScFv genes by SOE. The resulting ScFv genes were electrotransformed into E. coli TG1 and amplified with the co-infection of helper phage M13KO7 to obtain phage display library.

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Aim: To investigate the effect of capsaicin on IA and IK in cultured rat trigeminal ganglion (TG) neurons.

Methods: Whole-cell patch clamp technique was used to record the IA and IK before and after capsaicin perfusion at different concentrations.

Results: In capsaicin-sensitive (CS) neurons, capsaicin was shown to selectively inhibit IA in dose-dependent manner, the IC50 was 0.

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[Influence of PAF receptors on long term potentiation attenuated by aluminium in hippocampal CA3 area of rats].

Zhongguo Ying Yong Sheng Li Xue Za Zhi

May 2004

Department of Physiology, School of Basic Medical Sciences, Tongli Medical College, Wuhan 430030, China.

Aim: To investigate the influence of platelet-activating factor (PAF) receptor on long-term potentiation (LTP) attenuated by aluminium.

Methods: The method of extracellular recording was used to investigate the effect of PAF receptors on PP-CA3 LTP by microinjection of PAF receptor antagonist Ginkgolide B or agonist mc-PAF into CA3 area.

Results: (1) Amplitude of population spikes (PS) of evoked potential was not affected but LTP induction was blocked by 0.

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