Flexible Point-of-Care Electrodes for Ultrasensitive Detection of Bladder Tumor-Relevant miRNA in Urine.

Portable point-of-care testing (POCT) is currently drawing enormous attention owing to its great potential for disease diagnosis and personal health management. Electrochemical biosensors, with the intrinsic advantages of cost-effectiveness, fast response, ease of miniaturization, and integration, are considered as one of the most promising candidates for POCT application. However, the clinical application of electrochemical biosensors-based POCT is hindered by the decreased detection sensitivity due to the low abundance of disease-relevant biomolecules in extremely complex biological samples.

Association between Sleep Duration and Depression among the Elderly Population in China.

Objectives: The objective of this study was to understand how sleep duration could affect depression among elderly in China.

Method: A total of 7103 individuals aged 60 and older were selected from China Health and Retirement Longitudinal Study. A generalized linear mixed-effects model was used to estimate the relationship between sleep duration and depression, and we performed stratified analyses by age: young-old elderly, old-old elderly and oldest-old elderly.

[Forensic Analysis of 133 Cases of Knee Injuries].

Objective To discuss the types, characteristics, and the evaluation of disability of knee injuries. Methods The data of 133 cases of knee injury from 2017 to 2019 were collected and statistically analyzed according to the region of injury, the degree of disability, etc. Results One hundred and twenty-five cases of injury were compound, and 8 cases were simple.

[Electroacupuncture reduces obesity by improving metabolism and up-regulating expression of hypothalamic Sirtuin 1 and proopiomelanocortin in obese rats].

Objective: To observe the effect of electroacupuncture (EA) on expression of hypothalamic sirtuin 1(SIRT1) and proopiomelanocortin (POMC), and body weight, food-intake, blood glucose, and blood lipid levels in obese rats, so as to explore its mechanisms underlying improvement of obesity.

Methods: Forty male Wistar rats were randomly divided into normal, model, EA, and sham EA groups (＝10 rats in each group). The obesity model was established by feeding the rats with high fat diet.

[Current situation and thinking on the intestinal microflora regulation with acupuncture and moxibustion].

Large number of microflora is parasitized in the human intestinal tract, which maintains the stability of the microecological environment in the host's intestinal tract and the healthy state of the body. Once the steady state is out of balance, the intestinal microflora is dysfunctional and a variety of diseases will be induced. Acupuncture and moxibustion have a positive role in improving different types of clinical problems by regulating , harmonizing and , and strengthening the body's resistance.

Mechanistic Insights Into the Interaction Between Transcription Factors and Epigenetic Modifications and the Contribution to the Development of Obesity.

The development of obesity is inseparable from genetic and epigenetic factors, and transcription factors (TFs) play an essential role in these two mechanisms. This review analyzes the interaction of TFs with epigenetic modifications and the epigenetic mechanisms underlying peroxisome proliferator-activated receptor (PPAR)γ, an important transcription factor, in the development of obesity. We describe the relationship between TFs and different epigenetic modifications and illustrate the several mechanisms described.

Repair mechanism of mesenchymal stem cells derived from nasal mucosa in orbital fracture.

Objective: Mesenchymal stem cells (MSCs) derived from nasal mucosa are featured by high division and differentiation capacity, with large nuclei, obvious nucleoli and weak cytoplasmic basophily. Imaging examination, typically CT scan, is the gold standard for the diagnosis of orbital fracture.

Methods: We isolated MSCs derived from goat nasal mucosa and built the calcification model so as to investigate the repair mechanism of nasal mucosa-derived MSCs in orbital fracture.

[Prospects and Progress on the Mechanisms of Acupuncture Underlying Improvement in Diabetic Gastroparesis].

Diabetic gastroparesis (DGP) is an important complication of diabetes, and seriously impacts the patient's quality of life. In the present paper, the authors summarize national and international research on the mechanism of acupuncture therapy in improving DGP and the pathogenesis of DGP over the past 10 years. The authors illustrate the mechanism of acupuncture underlying the improvement in DGP as follows:1) the effect on autonomic nerves and neurons of the central nervous system; 2) promoting restoration of the enteric nervous system; 3) the effect on Cajal interstitial cells; 4) improving gastrointestinal hormone disorder; 5) improving hyperglycemic state; 6) the effect on gastric smooth muscle.

Mutagenic and Estrogenic Effects of Organic Compounds in Water Treated by Different Processes: A Pilot Study.

Objective: In this study, a pilot-scale investigation was conducted to examine and compare the biotoxicity of the organic compounds in effluents from five treatment processes (P1-P5) where each process was combination of preoxidation (O3), coagulation, sedimentation, sand filtration, ozonation, granular activated carbon, biological activated carbon and chlorination (NaClO).

Methods: Organic compounds were extracted by XAD-2 resins and eluted with acetone and dichlormethane (DCM). The eluents were evaporated and redissolved with DMSO or DCM.

Mechanisms Linking Inflammation to Insulin Resistance.

Obesity is now widespread around the world. Obesity-associated chronic low-grade inflammation is responsible for the decrease of insulin sensitivity, which makes obesity a major risk factor for insulin resistance and related diseases such as type 2 diabetes mellitus and metabolic syndromes. The state of low-grade inflammation is caused by overnutrition which leads to lipid accumulation in adipocytes.

[Arg-Gly-Asp-containing peptide combining with the biomimetic and modified PLGA-(ASP-PEG)].

Arg-Gly-Asp-(RGD) containing peptide characterized as the non-viral gene vector was synthesized to modify the surface of PLGA-(ASP-PEG). The Peptide (K16-GRGDSPC) was synthesized. PLGA-(ASP-PEG) was executed into chips A, B and C.

Induction of CML28-specific cytotoxic T cell responses using co-transfected dendritic cells with CML28 DNA vaccine and SOCS1 small interfering RNA expression vector.

CML28 is an attractive target for antigen-specific immunotherapy. SOCS1 represents an inhibitory control mechanism for DC antigen presentation and the magnitude of adaptive immunity. In this study, we evaluated the potential for inducing CML28-specific cytotoxic T lymphocytes (CTL) responses by dendritic cells (DCs)-based vaccination.

[Analysis and consider of technical identification for 32 cases medical tangle in medical association].

Objective: To analyze cause of medical accidents and actuality of technical identification in medical tangle.

Methods: 32 cases (17 death, 15 survive) of medical tangle by technical identification (according to sex, age, mostly diseases, sequel) and identified results (whether or not mistake, cause and effect connection, duty degree) have been studied.

Results: 13 cases of 32 medical accidents have been determined.

[Forensic identification for 16 cases with lumbar sacralization and lumbarization].

Lumbar sacralization and lumbarization are congenital spine malformation. 16 cases with lumbar sacralization or lumbarization were reviewed. Through studying the etiopathogenisis and clinical manifestation we analyze the relationship between injury and disease.

The inhibitory effects of deltamethrin on dopamine biosynthesis in rat PC12 cells.

The inhibitory effects of deltamethrin (DM) on dopamine biosynthesis in PC12 cells were investigated. Levels of dopamine in PC12 cells were detected after treatment with different doses from 10(-4)M to 10(-6)M deltamethrin or combined with 20 microM or 50 microM l-3,4-dihydroxyphenylalanine (L-Dopa) for 3 h or 12 h by HPLC. Flow cytometry (FCM) and RT-PCR technology were used to detect the levels of tyrosine hydroxylase (TH) mRNA and protein in PC12 cells treated with different doses of deltamethrin for 3 h or 12 h.

[Studies on the chemical constituents from the leaf of Liquidambar formosana Hance].

Objective: To study the chemical constituents from the methanolic extract from the leaf of Liquidambar formosana Hance.

Method: The compounds were separated with the column chromatographic and Prep HPLC, and their structures were identified by chemical and spectroscopic methods.

Results: Three compounds were isolated from the methanolic extract.

[Synthesis of a bio-active bone-matrix material and study of the cellular biocompatibility].

To prepare poly(lactic acid/glycolic acid/ asparagic acid-co- polyethylene glycol) (PLGA-[ASP-PEG]) and examine the cellular biocompatibility. PLGA-[ASP-PEG] was obtained by bulk ring-opening copolymerization method, examined by infrared spectrometry (IR) and 1H nuclear magnetic resonance spectroscopy (1H NMR). Bone marrow stromal cells(BMSCs) were cultured with PLGA-[ASP-PEG] (experiment gruop) and PLGA (control group) in vitro respectively, and were observed by phase-contrast microscopy and scanning electron microscopy.

[Marrow stromal cells and repair of bone defect].

Objective: To review researches on the relation between marrow stromal cells (MSCs) and repair of bone defect.

Methods: The latest original literatures about marrow stromal cells and their use in the treatment of bone defect were extensively reviewed.

Results: Marrow stromal cells were induced to osteoblasts under proper conditions and showed the potential of bone formation in vivo.

[The inhibitory effects of angiostatin on retinal neovascularization induced by oxygen].

Objective: To observe the inhibitory effects of angiostatin on microvascular endothelial cells of mouse retina and test the efficacy of native angiostatin in suppressing experimental retinal neovascularization induced by oxygen.

Methods: Angiostatin was purified with L-lysine Sepharose 4B from human plasma. The primary microvascular endothelial cells from rat retina were cultured.

[Effects of pyrethroids on dopamine and its metabolites in nigrostriatum of male rats].

Objective: To investigate the effects of pyrethroids on nigrostriatal dopaminergic pathways in male rats and its mechanism.

Methods: Different doses of permethrin (PM, 200, 400 mg/kg) and deltamethrin (DM, 6.25, 12.

[Effects of pyrethroids on tyrosine hydroxylase in nigrostriatum of male rats].

Objective: To study the effects of pyrethroids on tyrosine hydroxylase in nigrostriatum of male rats.

Methods: Different doses of permethrin and deltamethrin in corn oil were administered to rats by gavage for ten days, then PLC-Fluorescence detection was used to analyzed the contents of dopamine (DA), DOPAC and HVA in the Substantial Nigra and Striatum. The activities of TH were also detected by HPLC-Fluorescence detection.

[Tumor necrosis factor related apoptosis-inducing ligand induces apoptosis and sensitizes resistant hepatic cancer cells to chemotherapy].

Objective: To investigate the effective of tumor necrosis factor related apoptosis-inducing ligand (TRAIL) combination with chemotherapeutic agent inducing apoptosis in resistant hepatic cancer cells.

Methods: HepG2 cells resistant to Adriamycin (HepG2/ADR) were induced stepwise. The effects of TRAIL in combination with ADM (0.

Carboxymethylated chitin reduces MMP-1 expression in rabbit ACLT osteoarthritic cartilage.

Objective: To examine the in vivo effects of carboxymethylated chitin (CMC), intra-articularly administered, on cartilage degradation and the level and distribution of cartilage matrix metalloproteinase-1 (MMP-1).

Methods: Osteoarthritis (OA) was induced in 20 rabbits by unilateral anterior cruciate ligament transection (ACLT). The experimental group, comprising 10 rabbits randomly selected, was given an intra-articular injection of 0.

[Expression changes of tau protein and microtubule-associated proteins in testis intersitium of rats].

Objective: To examine the expression of the tau protein and microtubule-associated proteins in the testis interstitium of aged and young rats.

Methods: Sprague-Dawley male rats were divided into a young group(6 months, n = 10) and an aged group(28 months, n = 10). The two steps immunohistochemistry method with the antibody against tau protein and MAP alpha was performed on the testis tissues of the rats.

Apoptosis inducing effects of arsenic trioxide on human bladder cancer cell line BIU-87.

Objective: To explore the apoptosis inducing effects of arsenic trioxide (As2O3) on human bladder cancer cells and elucidate possible mechanisms.

Methods: After treatment with As2O3, the growth inhibition rates of human bladder cancer cell line BIU-87 were studied by MTT and cell counts methods. DNA synthesis rates were detected by 3H-TdR assay.