Bronchoscopic-Guided Selective Endobronchial Injection of Methylene Blue Solution for Localising Peripheral Bronchopleural Fistulas During Pleural Debridement in a Post-Lobectomy Patient With Bronchopleural Fistula and Empyema.

The bronchopleural fistula (BPF) is a pathological passageway between the bronchus and the pleural cavity. Diagnosing and localising BPF can be challenging, and the traditional retrograde methylene blue (MB) perfusion method may fail to identify multifocal BPFs. This article reports a novel method for locating multifocal BPFs in patients undergoing concurrent empyema debridement.

Global phylogeography and genetic characterization of carbapenem and ceftazidime-avibactam resistant KPC-33-producing Pseudomonas aeruginosa.

Ceftazidime-avibactam (CZA) is currently one of the last resorts used to treat infections caused by carbapenem-resistant Enterobacteriaceae and Pseudomonas aeruginosa. However, KPC variants have become the main mechanism mediating CZA resistance in KPC-producing gram-negative bacteria after increasing the application of CZA. Our previous study revealed that CZA-resistant KPC-33 had emerged in carbapenem-resistant P.

External validation of multiparametric magnetic resonance imaging-based decision rules for characterizing breast lesions and comparison to Kaiser score and breast imaging reporting and data system (BI-RADS) category.

Background: Breast imaging reporting and data system (BI-RADS) provides standard descriptors but not detailed decision rules for characterizing breast lesions. Diffusion-weighted imaging (DWI) and T2-weighted imaging (T2WI) are also not incorporated in the BI-RADS. Several multiparametric magnetic resonance imaging (mpMRI)-based decision rules have been developed to differentiate breast lesions, but lack external validation.

In vitro Antibacterial Effect of Reduning Combined with Polymyxin on Carbapenem Resistant .

Objective: This study aimed to investigate the status of carbapenem-resistant strains of isolated from the Department of Microbiology, Zhejiang Tongde Hospital between September 2023 and February 2024, and to examine the in vitro antibacterial effect of Reduning combined with polymyxin on carbapenem-resistant (CRKP), which may provide evidence on the application of Reduning in the clinical anti-infective therapy.

Methods: A total of 50 different isolates of CRKP were collected, and the minimum inhibitory concentrations (MIC) of polymyxin, Reduning and polymyxin plus Reduning were measured with microbroth dilution method. Then, the fractional inhibition concentration index (FICI) was calculated.

Clinical value of miR-200b in diagnosis and prognosis of childhood pneumonia.

Background: Childhood pneumonia (CP) is a common respiratory infectious disease in children with high morbidity and mortality. However, differential changes in miRNAs may interact with the regulation of CP. The aim of this paper was to discuss the miR-200b expression in CP and its diagnostic and prognostic value.

Immunogenic dying cells elicit potent anti-tumor T cell immunity against lung metastasis and tumorigenesis.

Purpose: Immune checkpoint blockades (ICBs) are promising, however they do not fit all types of tumor, such as those lack of tumor antigens. Induction of potent anti-tumor T cell immunity is critical for cancer therapy. In this study, we investigated the efficacy of immunotherapy via the immunogenic cell death (ICD) dying tumor cells in mouse models of lung metastasis and tumorigenesis.

Flavonoids of Diels et Gilg Against Acute Hepatic Injury by Blocking PI3K/AKT Signaling Pathway.

This study aims to investigate the mechanism of Diels et Gilg flavonoids (THF) on acute hepatic injury (AHI). First, high-performance liquid chromatography (HPLC) fingerprints were established to obtain the main chemical components of THF. According to the network pharmacology databases, collect active targets of AHI and potential targets.

Investigating causal relationships between plasma proteins and lung adenocarcinoma: result from proteomics and Mendelian randomization study.

Background: Plasma proteins contribute to the identification, diagnosis, and prognosis of human illnesses, which may be conducive to understanding the molecular mechanism and diagnosis of Lung adenocarcinoma (LUAD).

Methods: We collected plasma samples from 28 healthy individuals (H) and 56 LUAD patients and analyzed them using LC-MS/MS-based proteomics to determine differential expression plasma proteins (DEPPs). Then, the DEPPs were subjected to a two-sample Mendelian randomization (MR) study based on an "Inverse variance weighted (IVW)" approach to investigate the causal relationships between DEPPs and LUAD.

A novel MRI-based radiomics for preoperative prediction of lymphovascular invasion in rectal cancer.

Background: To develop and validate a clinical-radiomics model for preoperative prediction of lymphovascular invasion (LVI) in rectal cancer.

Methods: This retrospective study included data from 239 patients with pathologically confirmed rectal adenocarcinoma from two centers, all of whom underwent MRI examinations. Cases from the first center (n = 189) were randomly divided into a training set and an internal validation set at a 7:3 ratio, while cases from the second center (n = 50) constituted the external validation set.

Impact of time from blood specimens collection to centrifugation on the diagnosis of gestational diabetes mellitus.

Objective: The process of glycolysis from blood collection to centrifugation impacts the diagnosis of gestational diabetes mellitus (GDM). However, the specific characteristics of the working environment in China and its influence on GDM diagnosis still need to be clarified.

Methods: Firstly, 15 pregnant women were recruited, and six specimens were collected from each in a fasting state.

Disseminated organ and tissue infection secondary to carbapenem-resistant Klebsiella pneumoniae bloodstream infection for acute lymphoblastic leukemia treated with ceftazidime-avibactam: Two case reports.

Rationale: Carbapenem-resistant Klebsiella pneumoniae (CRKP) bloodstream infections are a severe complication resulting from granulocyte deficiency following chemotherapy for hematologic malignancies and have a high mortality rate. However, reports of disseminated organ infections secondary to bloodstream infections are rare.

Patient Concerns And Diagnoses: We report 2 cases of patients with acute lymphoblastic leukemia who both developed CRKP bloodstream infections during the granulocyte deficiency stage following chemotherapy, with 1 case of secondary bacterial liver abscess and 1 case of secondary septic arthritis.

Age-related changes in bioelectrical impedance analysis-derived phase angle (PhA) and the association between PhA and sarcopenic obesity in Chinese adults.

There are limited studies on the phase angle (PhA) and sarcopenic obesity (SO) in the Chinese population. This study aimed to establish 50 kHz-PhA reference data for SO population, and to evaluate the correlation between 50 kHz-PhA and SO. A total of 10,312 participants including 5415 men and 4897 women were enrolled in this study, and their resistance and reactance at 50 kHz, and body composition parameters were measured a segmental multifrequency bioelectrical impedance analysis device (InBody 720).

Alleviation of liver fibrosis by inhibiting a non-canonical ATF4-regulated enhancer program in hepatic stellate cells.

Liver fibrosis is a critical liver disease that can progress to more severe manifestations, such as cirrhosis, yet no effective targeted therapies are available. Here, we identify that ATF4, a master transcription factor in ER stress response, promotes liver fibrosis by facilitating a stress response-independent epigenetic program in hepatic stellate cells (HSCs). Unlike its canonical role in regulating UPR genes during ER stress, ATF4 activates epithelial-mesenchymal transition (EMT) gene transcription under fibrogenic conditions.

[Retrospective analysis of animal-related injuries in 1 266 patients based on the emergency department electronic medical record system].

Objective: To analyze the characteristics of animal-related injuries and summarize the epidemiological features of the affected population using the hospital's emergency department electronic medical record system.

Methods: A retrospective cross-sectional study was conducted, collecting data on animal-related injuries (dogs, cats, rodents, or other animals) as recorded in the outpatient registration system of the emergency department at Tongde Hospital of Zhejiang Province from October 8, 2022, to October 30, 2023. The study variables encompassed patient demographic characteristics (gender, age, occupation, residence, etc.

The impact of tamoxifen on apolipoproteins and lipoprotein(a) levels: an updated meta-analysis of randomized controlled trials.

Objective: The existing evidence regarding the impact of tamoxifen on lipoprotein(a) and apolipoproteins remains inconsistent. Therefore, this updated meta-analysis of randomized controlled trials (RCTs) aims to enhance the quality of evidence concerning the effects of tamoxifen on these lipid parameters.

Methods: Eligible RCTs published up to October 2024 were meticulously selected through a comprehensive search.

Interleukin-1 Receptor-Associated Kinase 4 (IRAK4) Degraders for Treating Inflammatory Diseases: Advances and Prospects.

Interleukin-1 receptor-associated kinase 4 (IRAK4) is involved in various inflammation-related diseases. Both the kinase and scaffolding functions of IRAK4 initiate pro-inflammatory factor transcription and expression. The scaffolding function of IRAK4 is essential for Myddosome assembly and NF-κB activation.

Matrine Inhibits Breast Cancer Cell Proliferation and Epithelial-Mesenchymal Transition Through Regulating the LINC01116/miR-9-5p/ITGB1 Axis.

Background: Breast cancer (BC) is the most prevalent solid cancer affecting women's health globally. Matrine (MAT), a traditional Chinese herb, has exhibited antitumor effects against BC. However, its mechanism of action, particularly whether it involves the control of cell proliferation and epithelial-mesenchymal transition (EMT), remains unknown.

Evaluation of the protective role of resveratrol on LPS-induced septic intestinal barrier function via TLR4/MyD88/NF-κB signaling pathways.

The intestinal barrier function is a critical defense mechanism in the human body, serving as both the primary target and initiating organ in cases of sepsis. Preserving the integrity of this barrier is essential for preventing complications and diseases, including sepsis and mortality. Despite this importance, the impact of resveratrol on intestinal barrier function remains unclear.

Functional connectivity gradients and neurotransmitter maps among patients with mild cognitive impairment and depression symptoms.

Background: Both depressive symptoms and neurotransmitter changes affect the characteristics of functional brain networks in clinical patients. We sought to explore how brain functional grading is organized among patients with mild cognitive impairment and depressive symptoms (D-MCI) and whether changes in brain organization are related to neurotransmitter distribution.

Methods: Using 3 T magnetic resonance imaging (MRI) we acquired functional MRI (fMRI) data from patients with D-MCI, patients with mild cognitive impairment without depression (nD-MCI), and healthy controls.

The Predictive Value of TyG Index and NLR for Risk of CHD and the Severity of Coronary Artery Lesions in Patients with Type 2 Diabetes Mellitus.

Background: There is a paucity of research examining the Triglyceride-glucose (TyG) index and Neutrophil-to-lymphocyte ratio (NLR) in relation to the onset and severity of coronary heart disease (CHD) in individuals with type 2 diabetes mellitus (T2DM). This study aims to explore the relationship between these biomarkers and the risk of CHD in T2DM patients and clarify their association with the CHD severity.

Methods: A total of 518 patients with T2DM who underwent coronary angiography (CAG) were included and categorized into either the T2DM group or the T2DM combined with CHD group based on the angiographic findings.

Association between the triglyceride-glucose index and liver fibrosis in adults with metabolism-related fatty liver disease in the United States: a cross-sectional study of NHANES 2017-2020.

Objective: This study aimed to examine the association between the triglyceride-glucose (TyG) index and liver fibrosis (LF) in U.S. adults with Metabolic Dysfunction-Associated Steatotic Liver Disease (MAFLD).

Therapeutic strategy and efficacy evaluation of chronic empyema after total pneumonectomy: individualized analysis of six patients.

Background: Chronic empyema after total pneumonectomy is a potentially fatal complication.The aim of the study is to explore the treatment strategy and clinical efficacy of chronic empyema after pneumonectomy.

Methods: A retrospective analysis of 6 patients with chronic empyema after pneumonectomy in our hospital.

Effects of Lycium barbarum Polysaccharide on Digestive Function and Intestinal Barrier Dysfunction in Septic Mice by Regulating JNK Pathway.

This study aimed to investigate the protective effects of Lycium barbarum polysaccharide (LBP) on digestive function and intestinal barrier integrity in septic mice, and to explore its underlying mechanisms. A total of 70 healthy male BALB/C mice were randomly assigned into five groups: blank control group (BG, n = 12), control group (CG, n = 12), low-dose group (LDG, n = 12, 200 mg/kg), medium-dose group (MDG, n = 12, 400 mg/kg), and high-dose group (HDG, n = 12, 800 mg/kg). A sepsis model was established by cecal ligation and puncture, followed by treatment with different doses of LBP.