24,292 results match your criteria: "Tokyo University of Agriculture & Technology 2-24-16 Naka-machi[Affiliation]"

Phenotyping and a genome-wide association study of elite lines of pearl millet.

Breed Sci

June 2024

Asian Research Center for Bioresource and Environmental Sciences (ARC-BRES), Graduate School of Agricultural and Life Sciences, The University of Tokyo, 1-1-1 Midori-cho, Nishitokyo-shi, Tokyo 188-0002, Japan.

Pearl millet ( (L.) R. BR.

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Article Synopsis
  • The study compared two culture systems for these parasites and found that static tank systems with water exchange offered better quality control than continuous flow-through systems.
  • Additionally, a minimally invasive biopsy method for estimating parasite loads was validated, allowing researchers to group fish with similar infections for more consistent experimental results.
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A homozygous calf with CACNA1S-related muscle weakness, a new autosomal recessive congenital disorder in Holstein cattle, was identified by genotyping 195 stored blood samples from Holstein calves aged less than 12 months. The patient was an 8-day-old male calf with congenital astasia which presented to a university hospital in 2019. The patient was unable to maintain an upright position with assistance to stand.

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RNA binding protein CUGBP2/ETR-3 regulates STAT3 alternative splicing.

Biochem Biophys Res Commun

December 2024

Laboratory of Molecular Medicinal Science, Department of Pharmaceutical Sciences, Ritsumeikan University, Shiga, Japan. Electronic address:

Signal transducer and activator of transcription 3 (STAT3) is a multifactorial regulator involved in many biological responses. Alternative splicing of STAT3 pre-mRNA leads to an internal 50-nucleotide deletion of exon 23 selecting an alternative 3' acceptor site, resulting in the generation of two splicing isoforms, STAT3α and STAT3β. STAT3β lacks 55 amino acid-residue transactivation domain at the C-terminal of STAT3α replacing seven unique amino acids.

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Phosphatidylcholine (PC), a key phospholipid, contains 2 fatty acids that can be bound at the sn-1 and sn-2 positions, resulting in positional isomers when different fatty acids are attached. Currently, there is no established method for identifying phospholipid molecular species and quantifying individual isomers using authentic standards of each PC isomer. In this study, we prepare authentic analytical standards for PC positional isomers through chemical synthesis and preparative purification.

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Enhanced nanobody-driven bioluminescent immunoassay for rapid parathion detection using engineered split-nanoluciferase.

Biosens Bioelectron

February 2025

Guangdong Provincial Key Laboratory of Food Quality and Safety, College of Food Science, South China Agricultural University, Guangzhou, Guangdong, 510642, China. Electronic address:

In this work, with parathion, a typical forbidden organophosphate pesticide as target drug, an enhanced nanobody-driven bioluminescent immunoassay based on the engineered split-nanoluciferase (NanoLuc) was proposed. Concretely, through labeling 11S and β10, two split-NanoLuc units onto the anti-parathion nanobody (Nb) VHH9 and the artificial antigen H1 coupled with carrier protein ovalbumin (H1-OVA) respectively, an NanoLuc Binary Technology (NanoBiT) system was firstly developed in the form of homogeneous immunoassay, in which the luminescence signal was produced by the reassembled NanoLuc after the combination of the 11S-fused VHH9 and β10-labeled H1-OVA. Subsequently, in order to enhance the signal-to-noise (S/N) ratio, a novel strategy of splitting 11S into two smaller subunits Δ11S and β9 was adopted so then an NanoLuc Ternary Technology (NanoTeT) system based on tri-part components of β9-fused VHH9, β10-labeled H1-OVA and Δ11S was successfully established.

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d-Galactofuranose (Galf) is widely distributed in glycoconjugates of pathogenic microbes. β-d-Galactofuranosidase (Galf-ase) from Streptomyces sp. JHA19 (ORF1110) belongs to glycoside hydrolase (GH) family 2 and is the first identified Galf-specific degradation enzyme.

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Freeze-dried fecal microorganisms as an effective biomaterial for the treatment of calves suffering from diarrhea.

Sci Rep

November 2024

International Education and Research Center for Food and Agricultural Immunology, Graduate School of Agricultural Science, Tohoku University, 468-1 Aoba, Aramaki, Aoba-Ku, Sendai, Miyagi, 980-8572, Japan.

Fecal microbiota transplantation (FMT) is a therapeutic modality for treating neonatal calf diarrhea. Several practical barriers, including donor selection, fecal collection, and a limited timeframe for FMT, are the main constraints to using fresh feces for implementing on-farm FMT. We report the utility of FMT with pretreated ready-to-use frozen (F) or freeze-dried (FD) microorganisms for treating calf diarrhea.

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Phased chromosome-level genome provides insights into the molecular adaptation for migratory lifestyle and population diversity for Pacific saury, Cololabis saira.

Commun Biol

November 2024

Research Centre for Deep Sea and Polar Fisheries, and Key Laboratory of Mariculture, Ministry of Education, Ocean University of China, Qingdao, China.

The Pacific saury (Cololabis saira) is a pelagic fish commonly found in the North Pacific Ocean. Its population diversity and migratory lifestyle have long captured global attention. Despite the inherent complexity of the C.

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Integration of biogas and liquid recirculation enhance methane production in high-solid anaerobic digestion of pig manure and rice husks.

Bioresour Technol

January 2025

Technical Department, Oriental Shiraishi Corporation, 5-6-52 Toyosu, Koto-ku, Tokyo 135-0061, Japan. Electronic address:

High-solid anaerobic digestion (HSAD) is a promising technology for treating livestock manure and agricultural waste; however, the wide application has been limited due to homogenization problems. This study established a novel HSAD system by integrating liquid and biogas recirculation (R), aiming to enhance the biogas conversion efficiency of high-solid substrates at low costs. Results showed that the highest methane yield was 357 mL/gVS in the novel HSAD system (R), 30.

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A PLIN1 polymorphism is associated with fat production in male emus.

Poult Sci

December 2024

Graduate School of Bioindustry, Tokyo University of Agriculture, Abashiri, Hokkaido 099-2493, Japan. Electronic address:

The emu (Dromaius novaehollandiae) is a novel poultry species that produces meat, eggs, and fat. Although emus have recently been domesticated, genetic improvements to establish strains have scarcely progressed. In this study, we investigated the relationship between production traits and perilipin 1-encoding gene (PLIN1) polymorphisms in the emus.

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Embryo culture is crucial to achieve successful outcomes in in vitro production-embryo transfer for cattle. This study explored the innovative use of dry incubators for bovine embryo culture, building on their advantages in human medicine, such as reduced contamination risk, stable temperature control, and lower gas consumption. In this study, we examined changes in osmotic pressure, the in vitro developmental potential of IVP embryos including the cleavage rate, blastocyst development rate, blastocyst diameter, and blastocyst cell number, morphokinetics, and the transcriptional profile of the blastocysts between humidified and dry incubators.

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Characterizing the role of PP2A B'' family subunits in mechanical stress response and plant development through calcium and ABA signaling in Arabidopsis thaliana.

PLoS One

November 2024

Asian Research Center for Bioresource and Environmental Sciences, Graduate School of Agricultural and Life Sciences, The University of Tokyo, Nishitokyo-shi, Tokyo, Japan.

Protein phosphatase 2AB'' (PP2A B'') family subunits have calcium-binding EF-hand motifs, facilitating interaction with PP2A substrates. In Arabidopsis thaliana, the PP2A B'' family subunits consist of six members, AtB''α-ε and FASS. These subunits can interact with a basic leucine zipper transcription factor, VIP1, and its close homologs.

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Article Synopsis
  • NG13 is a nitrogen-fixing bacterium that was isolated from the rhizosphere of rice in the early 1980s.
  • Its genome has been sequenced, revealing a single chromosome that is 5,897,538 base pairs long, with no plasmid present.
  • The classification of NG13 should be based on Average Nucleotide Identity (ANI) analysis.
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This study aimed to reveal individual differences between the speeds of the hand and ball and clarify the factors affecting those differences. Motion data from 20 highly skilled baseball pitchers, captured using a 16-camera motion analysis system with a sampling frequency of 1,000 hz, were used for correlation and hierarchical linear model analyses. For the hierarchical linear models, the speed of the metacarpophalangeal joint was the independent variable in the 1 level.

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Author Correction: Cryo-EM structures of ryanodine receptors and diamide insecticides reveal the mechanisms of selectivity and resistance.

Nat Commun

November 2024

Tianjin Key Laboratory for Modern Drug Delivery and High-Efficiency, Frontiers Science Center for Synthetic Biology, School of Pharmaceutical Science and Technology, Faculty of Medicine, Tianjin University, Tianjin, China.

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Gardenia blue (GB), a widely used plant-derived food color is prepared by reaction of genipin, the aglycone of geniposide, with protein hydrolysate. Recent animal studies investigating GB toxicity have indicated blue coloration in the gastrointestinal tract, kidneys and mesenteric lymph nodes in rodents following dietary administration. This study investigated the uptake and disposition of [C]GB in male and female rats and mice administered 100 or 1000 mg/kg by gavage.

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Genomic sequencing and neutralizing serological profiles during acute dengue infection: A 2017 cohort study in Nepal.

PLOS Glob Public Health

November 2024

Infectious and Viral Disease Research Laboratory, Central Department of Biotechnology, Tribhuvan University, Kirtipur, Kathmandu, Nepal.

Article Synopsis
  • Dengue virus (DENV), a major mosquito-borne threat, is prevalent in Nepal with limited knowledge on its evolution or immunity levels in the population.
  • A study of 49 patients during the 2017 dengue season revealed 43% tested positive for DENV NS1 antigen, indicating DENV2 as the dominant strain, with significant presence of neutralizing antibodies across various serotypes.
  • The findings highlight the need for enhanced local monitoring of DENV genotypes and immunity to better control future outbreaks in Nepal.
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Predation affects the evolution of sex-specific longevity.

Biol Lett

November 2024

Centre for Ecology & Conservation, Faculty of Environment, Science and Economy (ESE), University of Exeter, Cornwall Campus, Penryn, UK.

Predation, a major cause of natural selection, is classically thought to target the weak and sick. However, predators can target animals with condition-dependent sexual traits, and therefore, high-quality individuals can also be the focus of predation. Thus, it is not always clear which individuals are the foci of predators or how this affects trait evolution.

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Article Synopsis
  • - The study highlights that environmental areas, particularly international airports in Japan, are significant sites for the spread of antimicrobial resistance, specifically antibiotic-resistant bacteria.
  • - Findings revealed a concerning 46.3% prevalence of methicillin-resistant staphylococci on frequently touched surfaces, with specific strains carrying the mecA gene identified as major carriers.
  • - The research emphasizes the urgent need for molecular surveillance to detect and combat the spread of multidrug-resistant bacteria and antibiotic resistance in urban public spaces.
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A spherical green alga, , achieves phototaxis via a simple on/off switch of flagellar beating in response to changes in light intensity, without the need for complex signal transduction between cells. Moreover, the alga can change its susceptibility to light in order to adapt to its environment. To identify the mechanisms of susceptibility regulation, experiments were conducted at three different levels: population, individual, and cellular.

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"We are protectors, not protestors": global impacts of extractivism on human-nature bonds.

Sustain Sci

August 2024

Institute of Environmental Science and Technology (ICTA), Autonomous University of Barcelona, Edifici ICTA-ICP, Carrer de les Columnes S/N, Campus de la UAB, 08193 Cerdanyola del Vallès, Barcelona, Spain.

Unlabelled: This article analyzes the global impacts of extractivism on human-nature bonds. To do so, we rely on socio-ecological conflict data from the Global Atlas of Environmental Justice. Over 1800 cases involving resistance to the destruction of nature, cultures, cosmologies, worldviews, ancestral origins, and sacred places are analyzed using log-linear regression compared to 1600 cases that do not report such loss.

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The increase in the global population is leading to a doubling of the demand for protein. Soybean (), a key contributor to global plant-based protein supplies, requires ongoing yield enhancements to keep pace with increasing demand. Precise, on-plant seed counting and localization may catalyze breeding selection of shoot architectures and seed localization patterns related to superior performance in high planting density and contribute to increased yield.

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Phospholipid dynamics in Aspergillus species: relations between biological membrane composition and cellular morphology.

Biosci Biotechnol Biochem

November 2024

Department of Biotechnology, The University of Tokyo, Yayoi 1-1-1, Bunkyo-ku, Tokyo, Japan.

Biological membranes, primarily composed of phospholipid bilayers, are essential structures that compartmentalize the cell from the extracellular environment. The biosynthesis and regulation of membrane lipids have been extensively studied in model organisms such as Saccharomyces cerevisiae and mammalian cells. However, our understanding of biological membrane regulation in filamentous fungi, some of which are significant in medicine, pharmacy, and agriculture, remains limited.

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