300 results match your criteria: "Tianjin Institute of Environmental and Operational Medicine[Affiliation]"

π-HuB: the proteomic navigator of the human body.

Nature

December 2024

State Key Laboratory of Medical Proteomics, Beijing Proteome Research Center, National Center for Protein Sciences (Beijing), Beijing Institute of Lifeomics, Beijing, China.

The human body contains trillions of cells, classified into specific cell types, with diverse morphologies and functions. In addition, cells of the same type can assume different states within an individual's body during their lifetime. Understanding the complexities of the proteome in the context of a human organism and its many potential states is a necessary requirement to understanding human biology, but these complexities can neither be predicted from the genome, nor have they been systematically measurable with available technologies.

View Article and Find Full Text PDF

Detection of TNF-α using the established ab-MPs-CLIA.

Talanta

December 2024

State Key Laboratory of Toxicology and Medical Countermeasures, Beijing Institute of Pharmacology and Toxicology, Beijing, 100850, China. Electronic address:

Article Synopsis
View Article and Find Full Text PDF

Ethylbenzene (EB)-induced hepatotoxic effects has been indicated as oxidative damage and mitochondria-mediated apoptosis in vivo in our previous study, yet the mechanisms remain unclear. This study aimed to explore the role of the mTOR-p70S6K signaling pathway in EB-induced hepatoxic effects in vitro. Normal human hepatocytes (L02 cells) were exposed to different concentrations of ethylbenzene (0-10 mM) for 24 h.

View Article and Find Full Text PDF

Propylparaben Induces Reproductive Toxicity in Human Extravillous Trophoblast Cells via Apoptosis and Cell Cycle Pathways.

Environ Health (Wash)

May 2024

Tianjin Key Laboratory of Risk Assessment and Control Technology for Environment and Food Safety, Tianjin Institute of Environmental and Operational Medicine, Tianjin 300050, China.

Parabens (PBs), especially propylparaben, commonly used in consumer products, pose environmental and health concerns. This study explored propylparaben's cytotoxicity on HTR-8/SVneo human trophoblast cells, revealing significant dose-dependent cytotoxic effects, particularly post 48-h exposure. Elevated propylparaben levels triggered apoptosis, evidenced by increased Bax and activated Caspase-3, and induced the G0/G1 cell cycle arrest.

View Article and Find Full Text PDF

High altitude environment is mainly characterized by low oxygen. Due to persistent hypoxia, nonhealing wounds are common in high-altitude areas. Moreover, Basic fibroblast growth factor (bFGF) is a versatile biologically active substance that has crucial impact on wound healing.

View Article and Find Full Text PDF

CRISPR/Cas13a analysis based on NASBA amplification for norovirus detection.

Talanta

December 2024

Tianjin Key Laboratory of Risk Assessment and Control Technology for Environment and Food Safety, Tianjin Institute of Environmental and Operational Medicine, Tianjin, 300050, China. Electronic address:

Human norovirus (HuNoV) is a leading cause of foodborne diseases worldwide, making rapid and accurate detection crucial for prevention and control. In recent years, the CRISPR/Cas13a system, known for its single-base resolution in RNA recognition and unique collateral cleavage activity, is particularly suitable for sensitive and rapid RNA detection. However, isothermal amplification-based CRISPR/Cas13 assays often require an external transcription step, complicating the detection process.

View Article and Find Full Text PDF
Article Synopsis
  • Osteosarcoma is a serious type of bone cancer that's often hard to treat and can lead to death.
  • In this study, scientists focused on how low oxygen levels in the environment affect the cancer and found two markers, STC2 and TMEM45A, that can help predict how the disease behaves.
  • They also discovered that higher levels of STC2 were linked to worse outcomes in other types of cancer and that this marker is related to certain immune cells.
View Article and Find Full Text PDF

To alleviate bone loss, most current drugs target osteoclasts. Saikosaponin A (Ssa), a triterpene saponin derived from (also known as Radix bupleuri), has immunoregulatory, neuromodulatory, antiviral, anticancer, anti-convulsant, anti-inflammatory, and anti-proliferative effects. Recently, modulation of bone homeostasis was shown to involve ferroptosis.

View Article and Find Full Text PDF

Here, we explored the role of Prolyl 4-Hydroxylase Subunit Alpha 3 (P4HA3), the most recently identified member of the prolyl-4-hydroxylase (P4H) family, in head and neck squamous cell carcinoma (HNSCC) progression. P4HA3 is upregulated during cancer progression; however, its specific role in HNSCC progression remains elusive. Thus, this study aimed to elucidate the regulatory function of P4HA3 in HNSCC development and progression and to describe the underlying mechanisms.

View Article and Find Full Text PDF

Lam. leaves contain various nutrients and bioactive compounds. The present study aimed to assess the anti-fatigue capacity of a flavonoids concentrate purified from Lam.

View Article and Find Full Text PDF

Introduction: Bacterial resistance presents a major challenge to both the ecological environment and human well-being, with persistence playing a key role. Multiple studies were recently undertaken to examine the factors influencing the formation of persisters and the underlying process, with a primary focus on Gram-negative bacteria and (Gram-positive bacteria). Enterococcus faecalis () is capable of causing a variety of infectious diseases, but there have been few studies of persisters.

View Article and Find Full Text PDF

Pentachlorophenol (PCP) was once used as a pesticide, germicide, and preservative due to its stable properties and resistance to degradation. This study aimed to design a biosensor for the quantitative and prompt detection of capable of PCP. A cell-free fluorescence biosensor was developed while employing NalC, an allosteric Transcription Factor responsive to PCP and In Vitro Transcription.

View Article and Find Full Text PDF

A novel yeast-based biosensor for the quick determination of Deoxynivalenol.

Anal Chim Acta

August 2024

Tianjin Key Laboratory of Risk Assessment and Control Technology for Environment and Food Safety, Tianjin Institute of Environmental and Operational Medicine, Tianjin, 300050, PR China. Electronic address:

Mycotoxins are commonly found in food materials and severely threaten human health. Antibodies play a key role as a part of immunological techniques in detecting mycotoxins. Therefore, highly specific antibodies and detection techniques against mycotoxins need to be developed for advancements in medical research.

View Article and Find Full Text PDF

Background: Moringa oleifera leaves are rich in bioactive substances.

Purpose: The purpose of this study was to evaluate the effects of Moringa oleifera leaf aqueous extract supplements on energy metabolism and antioxidant function in young male adults.

Methods: Forty-four young male adults (26.

View Article and Find Full Text PDF

Microplastics have emerged as a prominent global environmental contaminant, and they have been found in both human placenta and breast milk. However, the potential effects and mechanisms of maternal exposure to microplastics at various gestational stages on offspring neurodevelopment remain poorly understood. This investigation delves into the potential neurodevelopmental ramifications of maternal exposure to polystyrene nanoplastics (PS-NPs) during distinct phases of pregnancy and lactation.

View Article and Find Full Text PDF

A smartphone-based fluorescent biosensor with metal-organic framework biocomposites and cotton swabs for the rapid determination of tetrodotoxin in seafood.

Anal Chim Acta

July 2024

School of Public Health, Binzhou Medical University, Yantai, 264003, China; Tianjin Key Laboratory of Risk Assessment and Control Technology for Environment and Food Safety, Tianjin Institute of Environmental and Operational Medicine, Tianjin, 300050, China. Electronic address:

Article Synopsis
  • Tetrodotoxin (TTX) is a highly toxic biotoxin found in puffer fish and some marine animals, posing risks to food safety and human health, and effective detection methods for it in seafood are urgently needed.
  • A new smartphone-based portable biosensor using metal-organic framework (MOF) biocomposites and cotton swabs has been developed for quick TTX detection, achieving impressive detection limits and ease of use.
  • This biosensor allows for rapid and accurate TTX determination in seafood samples, offering a practical tool for monitoring food safety that can be operated without specialized training in just 15 minutes.
View Article and Find Full Text PDF

Synthetic biology for the food industry: advances and challenges.

Crit Rev Biotechnol

February 2025

Tianjin Key Laboratory of Risk Assessment and Control Technology for Environment and Food Safety, Tianjin Institute of Environmental and Operational Medicine, Tianjin, China.

As global environmental pollution increases, climate change worsens, and population growth continues, the challenges of securing a safe, nutritious, and sustainable food supply have become enormous. This has led to new requirements for future food supply methods and functions. The use of synthetic biology technology to create cell factories suitable for food industry production and renewable raw material conversion into: important food components, functional food additives, and nutritional chemicals, represents an important method of solving the problems faced by the food industry.

View Article and Find Full Text PDF

Micro(nano)plastic, as a new type of environmental pollutant, have become a potential threat to the life and health of various stages of biology. However, it is not yet clear whether they will affect brain development in the fetal stage. Therefore, this study aims to explore the potential effects of nanoplastics on the development of fetal rat brains.

View Article and Find Full Text PDF

Nanopore sequencing is extremely promising for the high-throughput detection of pathogenic bacteria in natural water; these bacteria may be transmitted to humans and cause waterborne infectious diseases. However, the concentration of pathogenic bacteria in natural water is too low to be detected directly by nanopore sequencing. Herein, we developed a mica filter to enrich over 85% of bacteria from > 10 L of natural water in 100 min, which led to a 10-fold improvement in the assay limits of the MinION sequencer for assessing pathogenic bacteria.

View Article and Find Full Text PDF

In the present study, we address the limitations of conventional surface-enhanced Raman scattering (SERS) techniques for sensitive and stable detection of melamine in food products, especially dairy. To overcome these challenges, we developed a novel SERS-active substrate by incorporating gold nanoparticles (AuNPs) onto carboxyl-functionalized two-dimensional (2D) MXene material doped with nitrides, specifically Au-TiN-COOH. Our strategy leverages the unique physicochemical properties of MXene, a class of atomically thin, 2D transition metal carbides/nitrides, with tunable surface functionalities.

View Article and Find Full Text PDF

High-altitude exposure has been linked to cardiac dysfunction. Silent information regulator factor 2-related enzyme 1 (sirtuin 1, SIRT1), a nicotinamide adenine dinucleotide-dependent deacetylase, plays a crucial role in regulating numerous cardiovascular diseases. However, the relationship between SIRT1 and cardiac dysfunction induced by hypobaric hypoxia (HH) remains unexplored.

View Article and Find Full Text PDF

Rapid detection of melamine by DNA Walker mediated SERS sensing technique based on signal amplification function.

Mikrochim Acta

April 2024

Tianjin Key Laboratory of Risk Assessment and Control Technology for Environment and Food Safety, Tianjin Institute of Environmental and Operational Medicine, Tianjin, 300050, China.

Article Synopsis
  • A new way to find melamine (a chemical often found in food) using a technology called SERS has been developed.
  • This method uses special tools like gold magnets and small gold particles to help detect melamine levels more accurately.
  • It can find very tiny amounts (as low as 0.001 mg/kg) in food quickly and easily, making it useful for testing in real-world situations.
View Article and Find Full Text PDF

Chlorite and bromate alter the conjugative transfer of antibiotic resistance genes: Co-regulation of oxidative stress and energy supply.

J Hazard Mater

June 2024

Tianjin Institute of Environmental and Operational Medicine, Tianjin 300050, China; Key Laboratory of Risk Assessment and Control for Environment and Food Safety, Tianjin 300050, China. Electronic address:

The widespread use of disinfectants during the global response to the 2019 coronavirus pandemic has increased the co-occurrence of disinfection byproducts (DBPs) and antibiotic resistance genes (ARGs). Although DBPs pose major threats to public health globally, there is limited knowledge regarding their biological effects on ARGs. This study aimed to investigate the effects of two inorganic DBPs (chlorite and bromate) on the conjugative transfer of RP4 plasmid among Escherichia coli strains at environmentally relevant concentrations.

View Article and Find Full Text PDF